CERL1_ARATH
ID CERL1_ARATH Reviewed; 627 AA.
AC F4HVX7; F4HVX8; O23678;
DT 03-OCT-2012, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 60.
DE RecName: Full=Protein CER1-like 1;
GN OrderedLocusNames=At1g02190; ORFNames=T7I23.9;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP IDENTIFICATION, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Landsberg erecta;
RX PubMed=8718622; DOI=10.2307/3870155;
RA Aarts M.G., Keijzer C.J., Stiekema W.J., Pereira A.;
RT "Molecular characterization of the CER1 gene of arabidopsis involved in
RT epicuticular wax biosynthesis and pollen fertility.";
RL Plant Cell 7:2115-2127(1995).
RN [4]
RP IDENTIFICATION, AND TISSUE SPECIFICITY.
RX PubMed=21386033; DOI=10.1104/pp.111.172320;
RA Bourdenx B., Bernard A., Domergue F., Pascal S., Leger A., Roby D.,
RA Pervent M., Vile D., Haslam R.P., Napier J.A., Lessire R., Joubes J.;
RT "Overexpression of Arabidopsis ECERIFERUM1 promotes wax very-long-chain
RT alkane biosynthesis and influences plant response to biotic and abiotic
RT stresses.";
RL Plant Physiol. 156:29-45(2011).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=F4HVX7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=F4HVX7-2; Sequence=VSP_044265;
CC -!- TISSUE SPECIFICITY: Expressed in flowers and siliques. Not detected in
CC pollen, pedicels and seeds. {ECO:0000269|PubMed:21386033,
CC ECO:0000269|PubMed:8718622}.
CC -!- SIMILARITY: Belongs to the sterol desaturase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC24373.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; U89959; AAC24373.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE27398.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE27399.1; -; Genomic_DNA.
DR RefSeq; NP_171721.3; NM_100099.4. [F4HVX7-1]
DR RefSeq; NP_973742.1; NM_202013.1. [F4HVX7-2]
DR AlphaFoldDB; F4HVX7; -.
DR STRING; 3702.AT1G02190.1; -.
DR PaxDb; F4HVX7; -.
DR PRIDE; F4HVX7; -.
DR ProteomicsDB; 224481; -. [F4HVX7-1]
DR EnsemblPlants; AT1G02190.1; AT1G02190.1; AT1G02190. [F4HVX7-1]
DR EnsemblPlants; AT1G02190.2; AT1G02190.2; AT1G02190. [F4HVX7-2]
DR GeneID; 839541; -.
DR Gramene; AT1G02190.1; AT1G02190.1; AT1G02190. [F4HVX7-1]
DR Gramene; AT1G02190.2; AT1G02190.2; AT1G02190. [F4HVX7-2]
DR KEGG; ath:AT1G02190; -.
DR Araport; AT1G02190; -.
DR TAIR; locus:2204813; AT1G02190.
DR eggNOG; ENOG502QR3T; Eukaryota.
DR InParanoid; F4HVX7; -.
DR OMA; MSASRIC; -.
DR OrthoDB; 331955at2759; -.
DR PhylomeDB; F4HVX7; -.
DR PRO; PR:F4HVX7; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; F4HVX7; baseline and differential.
DR Genevisible; F4HVX7; AT.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0000254; F:C-4 methylsterol oxidase activity; IBA:GO_Central.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0016491; F:oxidoreductase activity; IBA:GO_Central.
DR GO; GO:0016126; P:sterol biosynthetic process; IBA:GO_Central.
DR InterPro; IPR006694; Fatty_acid_hydroxylase.
DR InterPro; IPR021940; Uncharacterised_Wax2_C.
DR Pfam; PF04116; FA_hydroxylase; 1.
DR Pfam; PF12076; Wax2_C; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..627
FT /note="Protein CER1-like 1"
FT /id="PRO_0000419615"
FT TRANSMEM 19..39
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 48..68
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 126..146
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 186..206
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 328..348
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 138..272
FT /note="Fatty acid hydroxylase"
FT /evidence="ECO:0000255"
FT VAR_SEQ 377..380
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_044265"
SQ SEQUENCE 627 AA; 72093 MW; 53921DD11F2D144B CRC64;
MASRPGALTE WPWSPLGSFK YLLVAPLVMA SMHSYVTAVD EEKDLSRLMI VVLMLWRIVH
SQIWISVSRQ RTAKGTNKIV DKPIEFEQVD RERTWDDQVI FNTLLMYLAN IKLPGASHLP
PWRLDGAILM ALLHAGPVEF LYYWFHRALH HHFLYSRYHS HHHSSIVTEP ITSVVHPFAE
HIAYTLLFAI PMVTASLCGI LSIVSIMGYI TYIDFMNNMG HCNFELFPKR LFHLFPPLKF
LCYTPSFHSL HHTQFRTNYS LFMPIYDFIY GTTDNLTDSL YERSLEIEEE SPDVIHLTHL
TTHNSIYQMR LGFPSLSSCP LWSRPPWYLT CFMWPFTLLC SFALTSAIPL RTFVFERNRL
RDLTVHSHLL PKFSFHYKSQ RHHESINTII EEAILEADEK GVKVMSLGLM NNREELNGSG
EMYVQKYPKL KIRLVDGSSM AATVVINNIP KEATEIVFRG NLTKVASAVV FALCQKGVKV
VVLREEEHSK LIKSGVDKNL VLSTSNSYYS PKVWLVGDGI ENEEQMKAKE GTLFVPFSHF
PPNKLRKDCF YQSTPAMRVP KSAQNIDSCE NWLGRRVMSA WKIGGIVHAL EGWEEHDCGN
TCNVLRLHAI WEAALRHDFQ PLPPSPL
