CERL2_ARATH
ID CERL2_ARATH Reviewed; 613 AA.
AC F4IR05; B3H5B0; O80938;
DT 03-OCT-2012, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 25-MAY-2022, entry version 62.
DE RecName: Full=Protein CER1-like 2;
GN OrderedLocusNames=At2g37700; ORFNames=F13M22.20;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP IDENTIFICATION, AND TISSUE SPECIFICITY.
RX PubMed=21386033; DOI=10.1104/pp.111.172320;
RA Bourdenx B., Bernard A., Domergue F., Pascal S., Leger A., Roby D.,
RA Pervent M., Vile D., Haslam R.P., Napier J.A., Lessire R., Joubes J.;
RT "Overexpression of Arabidopsis ECERIFERUM1 promotes wax very-long-chain
RT alkane biosynthesis and influences plant response to biotic and abiotic
RT stresses.";
RL Plant Physiol. 156:29-45(2011).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=F4IR05-1; Sequence=Displayed;
CC Name=2;
CC IsoId=F4IR05-2; Sequence=VSP_044266, VSP_044267;
CC -!- TISSUE SPECIFICITY: Not detected in any tissues.
CC {ECO:0000269|PubMed:21386033}.
CC -!- SIMILARITY: Belongs to the sterol desaturase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC23640.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC004684; AAC23640.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC09436.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC09437.1; -; Genomic_DNA.
DR PIR; T02536; T02536.
DR RefSeq; NP_001118464.1; NM_001124992.2. [F4IR05-2]
DR RefSeq; NP_181306.3; NM_129326.3. [F4IR05-1]
DR AlphaFoldDB; F4IR05; -.
DR STRING; 3702.AT2G37700.1; -.
DR PaxDb; F4IR05; -.
DR PRIDE; F4IR05; -.
DR ProteomicsDB; 224482; -. [F4IR05-1]
DR EnsemblPlants; AT2G37700.1; AT2G37700.1; AT2G37700. [F4IR05-1]
DR EnsemblPlants; AT2G37700.2; AT2G37700.2; AT2G37700. [F4IR05-2]
DR GeneID; 818347; -.
DR Gramene; AT2G37700.1; AT2G37700.1; AT2G37700. [F4IR05-1]
DR Gramene; AT2G37700.2; AT2G37700.2; AT2G37700. [F4IR05-2]
DR KEGG; ath:AT2G37700; -.
DR Araport; AT2G37700; -.
DR TAIR; locus:2040666; AT2G37700.
DR eggNOG; ENOG502QR3T; Eukaryota.
DR HOGENOM; CLU_017842_1_0_1; -.
DR InParanoid; F4IR05; -.
DR PRO; PR:F4IR05; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; F4IR05; baseline and differential.
DR Genevisible; F4IR05; AT.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0000254; F:C-4 methylsterol oxidase activity; IBA:GO_Central.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0016491; F:oxidoreductase activity; IBA:GO_Central.
DR GO; GO:0016126; P:sterol biosynthetic process; IBA:GO_Central.
DR InterPro; IPR006694; Fatty_acid_hydroxylase.
DR InterPro; IPR021940; Uncharacterised_Wax2_C.
DR Pfam; PF04116; FA_hydroxylase; 1.
DR Pfam; PF12076; Wax2_C; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..613
FT /note="Protein CER1-like 2"
FT /id="PRO_0000419616"
FT TRANSMEM 13..33
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 44..64
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 95..115
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 122..142
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 182..202
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 322..342
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 134..268
FT /note="Fatty acid hydroxylase"
FT /evidence="ECO:0000255"
FT VAR_SEQ 473..493
FT /note="MVLRKEEHSMLAEFLDDKCKE -> PSFSTNGESETKGFNLDGPFL (in
FT isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_044266"
FT VAR_SEQ 494..613
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_044267"
SQ SEQUENCE 613 AA; 70573 MW; 2B4301AF2C128E65 CRC64;
MASRPGFLTD WPWTPLGSFK YLLLAPLVFD SIYSYATIRD HEKLLIVAVT VWRIVHSQIW
ISLSRYQTAK GTKRILNKSI EFDQVDRERT WDDQIIFNTL IVYLTKVYVS GTSTIPFWRT
DGVILVALLH AGPVEFIYYW FHRALHHHFL YSRYHSHHHS SIVTEPITSV VHPFAEHIGY
TLILGLPLIT TFMCGTVSVV SIALYLTYID FMNNMGHCNF ELIPKFLFSL LPPLKFLCYT
PSFHSLHHTQ FRTNYSLFMP MYDYIYGTTD ECSDSLYETS LEKEEEKPDA IHLTHLTSLD
SIYHLRLGFA SLSSHPLSSR CYLFLMKPFA LILSFILRSF SFQTFVVERN RFRDLTLHSH
LLPKFSSHYM SHQQKECINK MIEAAILEAD KKGVKVMSLG LLNQGEELNG YGEMYVRRHP
KLKIRIVDGG SLAAEVVLHS IPVGTKEVLF RGQITKVARA IVFSLCQNAI KVMVLRKEEH
SMLAEFLDDK CKENLIWLVG DGLSTKEQKM AKDGTLFLPF SQFPPKTLRK DCFYHTTPAM
IIPHSAQNID SCENWLGRRV MSAWRVGGIV HALEGWKEHE CGLDDNSIIN PPRVWEAALR
NGFQPLLLPS LET
