CESS3_CONMO
ID CESS3_CONMO Reviewed; 101 AA.
AC A0A4Y5X1A7;
DT 07-OCT-2020, integrated into UniProtKB/Swiss-Prot.
DT 18-SEP-2019, sequence version 1.
DT 25-MAY-2022, entry version 9.
DE RecName: Full=Conopressin/conophysin, isoform 3 {ECO:0000303|PubMed:32058072};
DE Contains:
DE RecName: Full=Conopressin-K {ECO:0000303|PubMed:32058072};
DE Contains:
DE RecName: Full=Conophysin {ECO:0000303|PubMed:32058072};
DE Flags: Precursor; Fragment;
OS Conus monile (Necklace cone).
OC Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC Caenogastropoda; Neogastropoda; Conoidea; Conidae; Conus; Strategoconus.
OX NCBI_TaxID=351660;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 2-10 AND 14-68, AMIDATION
RP AT GLY-10, IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION, AND
RP SYNTHESIS OF 2-10.
RC TISSUE=Venom, and Venom duct;
RX PubMed=32058072; DOI=10.1016/j.bbapap.2020.140391;
RA Kumar S., Vijayasarathy M., Venkatesha M.A., Sunita P., Balaram P.;
RT "Cone snail analogs of the pituitary hormones oxytocin/vasopressin and
RT their carrier protein neurophysin. Proteomic and transcriptomic
RT identification of conopressins and conophysins.";
RL Biochim. Biophys. Acta 1868:140391-140391(2020).
CC -!- FUNCTION: [Conopressin-K]: Targets vasopressin-oxytocin related
CC receptors. Is more active on fish receptors than on their human
CC counterparts, supporting an evolved role of this conopressin in the
CC envenomation process. Acts as an agonist on zebrafish vasopressin
CC receptors V1a1R (EC(50)=10.6 nM), V1a2R (EC(50)=44.06 nM, partial
CC agonist), V2R (EC(50)=299.2 nM) and oxytocin receptor (EC(50)=353.73
CC nM, partial agonist). Shows a weaker activity on human receptors AVPR1B
CC (EC(50)=51.92 nM), AVPR1A (EC(50)=123.78 nM), AVPR2 (EC(50)=299.2 nM)
CC and oxytocin (OXTR) receptor (EC(50)=455.66 nM, partial agonist). In
CC vivo, exhibits grooming and scratching behavior in mice, following
CC intracerebral injection. {ECO:0000250|UniProtKB:P05486}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:32058072}.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC {ECO:0000305|PubMed:32058072}.
CC -!- DOMAIN: The cysteine framework of the conopressin is C-C.
CC {ECO:0000305}.
CC -!- MASS SPECTROMETRY: [Conophysin]: Mass=9419.76; Method=Electrospray;
CC Note=Monoisotopic mass.; Evidence={ECO:0000269|PubMed:32058072};
CC -!- MASS SPECTROMETRY: [Conophysin]: Mass=9435.7; Method=Electrospray;
CC Note=Monoisotopic mass of protein with probable hydroxyPro.;
CC Evidence={ECO:0000269|PubMed:32058072};
CC -!- MASS SPECTROMETRY: [Conophysin]: Mass=9463.7; Method=Electrospray;
CC Note=Monoisotopic mass of protein with probable gamma-carboxylation.;
CC Evidence={ECO:0000269|PubMed:32058072};
CC -!- MASS SPECTROMETRY: [Conopressin-K]: Mass=1033.52; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:32058072};
CC -!- MISCELLANEOUS: The letter 'K' in the name 'Conopressin-K' proposed by
CC Kumar and colleagues stands for the eighth residue, which differs
CC between conopressins. {ECO:0000305|PubMed:32058072}.
CC -!- SIMILARITY: Belongs to the vasopressin/oxytocin family. {ECO:0000305}.
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DR EMBL; MK263338; QDE14046.1; -; mRNA.
DR AlphaFoldDB; A0A4Y5X1A7; -.
DR SMR; A0A4Y5X1A7; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0005185; F:neurohypophyseal hormone activity; IEA:InterPro.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR Gene3D; 2.60.9.10; -; 1.
DR InterPro; IPR000981; Neurhyp_horm.
DR InterPro; IPR036387; Neurhyp_horm_dom_sf.
DR InterPro; IPR022423; Neurohypophysial_hormone_CS.
DR PANTHER; PTHR11681; PTHR11681; 1.
DR Pfam; PF00184; Hormone_5; 1.
DR PRINTS; PR00831; NEUROPHYSIN.
DR SMART; SM00003; NH; 1.
DR SUPFAM; SSF49606; SSF49606; 1.
DR PROSITE; PS00264; NEUROHYPOPHYS_HORM; 1.
PE 1: Evidence at protein level;
KW Amidation; Cleavage on pair of basic residues; Direct protein sequencing;
KW Disulfide bond; G-protein coupled receptor impairing toxin; Secreted;
KW Signal; Toxin.
FT SIGNAL <1..1
FT /evidence="ECO:0000250|UniProtKB:P05486"
FT CHAIN 2..101
FT /note="Conopressin/conophysin, isoform 3"
FT /id="PRO_0000450740"
FT PEPTIDE 2..10
FT /note="Conopressin-K"
FT /evidence="ECO:0000269|PubMed:32058072"
FT /id="PRO_0000450741"
FT PROPEP 11..18
FT /evidence="ECO:0000250|UniProtKB:P05486"
FT /id="PRO_0000450742"
FT CHAIN 14..101
FT /note="Conophysin"
FT /evidence="ECO:0000269|PubMed:32058072"
FT /id="PRO_0000450743"
FT MOD_RES 10
FT /note="Glycine amide"
FT /evidence="ECO:0000269|PubMed:32058072"
FT DISULFID 2..7
FT /evidence="ECO:0000250|UniProtKB:P05486"
FT DISULFID 23..63
FT /evidence="ECO:0000250|UniProtKB:P01175"
FT DISULFID 26..37
FT /evidence="ECO:0000250|UniProtKB:P01175"
FT DISULFID 31..53
FT /evidence="ECO:0000250|UniProtKB:P01175"
FT DISULFID 38..43
FT /evidence="ECO:0000250|UniProtKB:P01175"
FT DISULFID 70..88
FT /evidence="ECO:0000250|UniProtKB:P01175"
FT DISULFID 82..100
FT /evidence="ECO:0000250|UniProtKB:P01175"
FT DISULFID 89..94
FT /evidence="ECO:0000250|UniProtKB:P01175"
FT NON_TER 1
SQ SEQUENCE 101 AA; 10873 MW; EBED4AF79E41B873 CRC64;
ACFIRNCPKG GKRNVDEGPT KPCMFCSFGQ CVAPHTCCGE KGCEMGTVDA NMCQEENESP
IPCHVFGKRC LLNHPGNSHG NCVTYGICCS HDTCTVHLAC M
