CESS_CONGE
ID CESS_CONGE Reviewed; 128 AA.
AC P05486; X5I9X9;
DT 01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT 09-DEC-2015, sequence version 2.
DT 25-MAY-2022, entry version 76.
DE RecName: Full=Conopressin-conophysin {ECO:0000305};
DE Contains:
DE RecName: Full=Conopressin-G {ECO:0000303|PubMed:24662800};
DE Short=Con-G {ECO:0000303|PubMed:32155768};
DE AltName: Full=Conopressin-K {ECO:0000250|UniProtKB:A0A4Y5X1A7};
DE AltName: Full=Lys-conopressin-G {ECO:0000303|PubMed:3680228};
DE Contains:
DE RecName: Full=Conophysin-G {ECO:0000303|PubMed:24662800};
DE Flags: Precursor;
OS Conus geographus (Geography cone) (Nubecula geographus).
OC Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC Caenogastropoda; Neogastropoda; Conoidea; Conidae; Conus; Gastridium.
OX NCBI_TaxID=6491;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Venom duct;
RX PubMed=24662800; DOI=10.1038/ncomms4521;
RA Dutertre S., Jin A.-H., Vetter I., Hamilton B., Sunagar K., Lavergne V.,
RA Dutertre V., Fry B.G., Antunes A., Venter D.J., Alewood P.F., Lewis R.J.;
RT "Evolution of separate predation- and defence-evoked venoms in carnivorous
RT cone snails.";
RL Nat. Commun. 5:3521-3521(2014).
RN [2]
RP PROTEIN SEQUENCE OF 28-36, FUNCTION, BIOASSAY, AMIDATION AT GLY-36,
RP SUBCELLULAR LOCATION, AND DISULFIDE BOND.
RC TISSUE=Venom;
RX PubMed=3680228; DOI=10.1016/s0021-9258(18)47661-2;
RA Cruz L.J., de Santos V., Zafaralla G.C., Ramilo C.A., Zeikus R.D.,
RA Gray W.R., Olivera B.M.;
RT "Invertebrate vasopressin/oxytocin homologs. Characterization of peptides
RT from Conus geographus and Conus striatus venoms.";
RL J. Biol. Chem. 262:15821-15824(1987).
RN [3]
RP STRUCTURE BY NMR OF 28-36, AND FUNCTION.
RX PubMed=32155768; DOI=10.3390/md18030150;
RA Giribaldi J., Ragnarsson L., Pujante T., Enjalbal C., Wilson D., Daly N.L.,
RA Lewis R.J., Dutertre S.;
RT "Synthesis, pharmacological and structural characterization of novel
RT conopressins from Conus miliaris.";
RL Mar. Drugs 18:0-0(2020).
CC -!- FUNCTION: [Conopressin-G]: Targets vasopressin-oxytocin related
CC receptors (PubMed:32155768). Is more active on fish receptors than on
CC their human counterparts, supporting an evolved role of this
CC conopressin in the envenomation process (PubMed:32155768). Acts as an
CC agonist on zebrafish vasopressin receptors V1a1R (EC(50)=10.6 nM),
CC V1a2R (EC(50)=44.06 nM, partial agonist), V2R (EC(50)=299.2 nM) and
CC oxytocin receptor (EC(50)=353.73 nM, partial agonist)
CC (PubMed:32155768). Shows a weaker activity on human receptors AVPR1B
CC (EC(50)=51.92 nM), AVPR1A (EC(50)=123.78 nM), AVPR2 (EC(50)=299.2 nM)
CC and oxytocin (OXTR) receptor (EC(50)=455.66 nM, partial agonist). In
CC vivo, exhibits grooming and scratching behavior in mice, following
CC intracerebral injection (PubMed:3680228). {ECO:0000269|PubMed:32155768,
CC ECO:0000269|PubMed:3680228}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:3680228}.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC {ECO:0000305|PubMed:24662800}.
CC -!- DOMAIN: The cysteine framework of the conopressin is C-C.
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the vasopressin/oxytocin family. {ECO:0000305}.
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DR EMBL; AB910804; BAO65572.1; -; mRNA.
DR PIR; A28495; A28495.
DR AlphaFoldDB; P05486; -.
DR SMR; P05486; -.
DR ConoServer; 1266; conopressin-G.
DR ConoServer; 8951; conopressin-G.
DR ConoServer; 8953; conopressin-G.
DR ConoServer; 8954; conopressin-G.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0005185; F:neurohypophyseal hormone activity; IEA:InterPro.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR Gene3D; 2.60.9.10; -; 1.
DR InterPro; IPR000981; Neurhyp_horm.
DR InterPro; IPR036387; Neurhyp_horm_dom_sf.
DR InterPro; IPR022423; Neurohypophysial_hormone_CS.
DR PANTHER; PTHR11681; PTHR11681; 1.
DR Pfam; PF00184; Hormone_5; 1.
DR PRINTS; PR00831; NEUROPHYSIN.
DR SMART; SM00003; NH; 1.
DR SUPFAM; SSF49606; SSF49606; 1.
DR PROSITE; PS00264; NEUROHYPOPHYS_HORM; 1.
PE 1: Evidence at protein level;
KW Amidation; Direct protein sequencing; Disulfide bond;
KW G-protein coupled receptor impairing toxin; Secreted; Signal; Toxin.
FT SIGNAL 1..27
FT /evidence="ECO:0000255"
FT PEPTIDE 28..36
FT /note="Conopressin-G"
FT /evidence="ECO:0000269|PubMed:3680228"
FT /id="PRO_0000044098"
FT PROPEP 37..44
FT /evidence="ECO:0000305"
FT /id="PRO_0000435032"
FT CHAIN 45..128
FT /note="Conophysin-G"
FT /id="PRO_0000435033"
FT MOD_RES 36
FT /note="Glycine amide"
FT /evidence="ECO:0000269|PubMed:3680228"
FT DISULFID 28..33
FT /evidence="ECO:0000269|PubMed:3680228"
FT DISULFID 50..90
FT /evidence="ECO:0000250|UniProtKB:P01175"
FT DISULFID 53..64
FT /evidence="ECO:0000250|UniProtKB:P01175"
FT DISULFID 58..80
FT /evidence="ECO:0000250|UniProtKB:P01175"
FT DISULFID 65..70
FT /evidence="ECO:0000250|UniProtKB:P01175"
FT DISULFID 97..115
FT /evidence="ECO:0000250|UniProtKB:P01175"
FT DISULFID 109..127
FT /evidence="ECO:0000250|UniProtKB:P01175"
FT DISULFID 116..121
FT /evidence="ECO:0000250|UniProtKB:P01175"
SQ SEQUENCE 128 AA; 13875 MW; 8E5916B8FCD844D8 CRC64;
MTRSAMQMGR LTLVLCLLLL LLLTTQACFI RNCPKGGKRD VDERYLFKAC MSCSFGQCVG
PRICCGPRGC EMGTAEANRC IEEDEDPIPC QVVGQHCDLN NPGNIHGNCV ANGICCVDDT
CTIHTGCL
