ID   CEST_ECO27              Reviewed;         156 AA.
AC   P21244; B7UM98;
DT   01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1991, sequence version 1.
DT   25-MAY-2022, entry version 96.
DE   RecName: Full=Tir chaperone;
GN   Name=cesT; OrderedLocusNames=E2348C_3940;
OS   Escherichia coli O127:H6 (strain E2348/69 / EPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=574521;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=E2348/69 / EPEC;
RX   PubMed=2172966; DOI=10.1073/pnas.87.20.7839;
RA   Jerse A.E., Yu J., Tall B.D., Kaper J.B.;
RT   "A genetic locus of enteropathogenic Escherichia coli necessary for the
RT   production of attaching and effacing lesions on tissue culture cells.";
RL   Proc. Natl. Acad. Sci. U.S.A. 87:7839-7843(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=E2348/69 / EPEC;
RX   PubMed=9593291; DOI=10.1046/j.1365-2958.1998.00783.x;
RA   Elliott S.J., Wainwright L.A., McDaniel T.K., Jarvis K.G., Deng Y.K.,
RA   Lai L.C., McNamara B.P., Donnenberg M.S., Kaper J.B.;
RT   "The complete sequence of the locus of enterocyte effacement (LEE) from
RT   enteropathogenic Escherichia coli E2348/69.";
RL   Mol. Microbiol. 28:1-4(1998).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=E2348/69 / EPEC;
RX   PubMed=18952797; DOI=10.1128/jb.01238-08;
RA   Iguchi A., Thomson N.R., Ogura Y., Saunders D., Ooka T., Henderson I.R.,
RA   Harris D., Asadulghani M., Kurokawa K., Dean P., Kenny B., Quail M.A.,
RA   Thurston S., Dougan G., Hayashi T., Parkhill J., Frankel G.;
RT   "Complete genome sequence and comparative genome analysis of
RT   enteropathogenic Escherichia coli O127:H6 strain E2348/69.";
RL   J. Bacteriol. 191:347-354(2009).
RN   [4]
RP   CHARACTERIZATION.
RX   PubMed=10510232; DOI=10.1046/j.1365-2958.1999.01559.x;
RA   Elliott S.J., Hutcheson S.W., Dubois M.S., Mellies J.L., Wainwright L.A.,
RA   Batchelor M., Frankel G., Knutton S., Kaper J.B.;
RT   "Identification of CesT, a chaperone for the type III secretion of Tir in
RT   enteropathogenic Escherichia coli.";
RL   Mol. Microbiol. 33:1176-1189(1999).
RN   [5]
RP   CHARACTERIZATION.
RX   PubMed=10510231; DOI=10.1046/j.1365-2958.1999.01558.x;
RA   Abe A., de Grado M., Pfuetzner R.A., Sanchez-Sanmartin C., Devinney R.,
RA   Puente J.L., Strynadka N.C., Finlay B.B.;
RT   "Enteropathogenic Escherichia coli translocated intimin receptor, Tir,
RT   requires a specific chaperone for stable secretion.";
RL   Mol. Microbiol. 33:1162-1175(1999).
CC   -!- FUNCTION: Chaperone for the type III secretion of Tir. Probably
CC       stabilizes the protein, prevents inappropriate protein-
CC       proteininteractions and aids in secretion.
CC   -!- INTERACTION:
CC       P21244; B7UM99: tir; NbExp=6; IntAct=EBI-2504434, EBI-2504426;
CC       P21244; O52142: D3G36_10545; Xeno; NbExp=2; IntAct=EBI-2504434, EBI-6419814;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the CesT/SycH chaperone family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; M58154; AAA62774.1; -; Genomic_DNA.
DR   EMBL; AF022236; AAC38391.1; -; Genomic_DNA.
DR   EMBL; FM180568; CAS11488.1; -; Genomic_DNA.
DR   PIR; I41196; I41196.
DR   RefSeq; WP_000098791.1; NC_011601.1.
DR   AlphaFoldDB; P21244; -.
DR   BMRB; P21244; -.
DR   SMR; P21244; -.
DR   IntAct; P21244; 4.
DR   EnsemblBacteria; CAS11488; CAS11488; E2348C_3940.
DR   KEGG; ecg:E2348C_3940; -.
DR   HOGENOM; CLU_1683457_0_0_6; -.
DR   OMA; TNDEYMM; -.
DR   Proteomes; UP000008205; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0030254; P:protein secretion by the type III secretion system; IEA:InterPro.
DR   InterPro; IPR010261; Tir_chaperone.
DR   Pfam; PF05932; CesT; 1.
PE   1: Evidence at protein level;
KW   Chaperone; Cytoplasm; Virulence.
FT   CHAIN           1..156
FT                   /note="Tir chaperone"
FT                   /id="PRO_0000216361"
SQ   SEQUENCE   156 AA;  17707 MW;  1E9F7ED2B9D5F1DF CRC64;
     MSSRSELLLD RFAEKIGVGS ISFNENRLCS FAIDEIYYIS LSDANDEYMM IYGVCGKFPT
     DNPNFALEIL NANLWFAENG GPYLCYESGA QSLLLALRFP LDDATPEKLE NEIEVVVKSM
     ENLYLVLHNQ GITLENEHMK IEEISSSDNK HYYAGR