CEST_ECO57
ID CEST_ECO57 Reviewed; 156 AA.
AC P58233;
DT 29-AUG-2001, integrated into UniProtKB/Swiss-Prot.
DT 29-AUG-2001, sequence version 1.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=Tir chaperone;
GN Name=cesT; OrderedLocusNames=Z5111, ECs4560; ORFNames=L0026;
OS Escherichia coli O157:H7.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83334;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=O157:H7 / HA1 / EHEC;
RX PubMed=8566710; DOI=10.1111/j.1574-6968.1995.tb07856.x;
RA Zhao S., Mitchell S.E., Meng J., Doyle M.P., Kresovich S.;
RT "Cloning and nucleotide sequence of a gene upstream of the eaeA gene of
RT enterohemorrhagic Escherichia coli O157:H7.";
RL FEMS Microbiol. Lett. 133:35-39(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX PubMed=9673266; DOI=10.1128/iai.66.8.3810-3817.1998;
RA Perna N.T., Mayhew G.F., Posfai G., Elliott S., Donnenberg M.S.,
RA Kaper J.B., Blattner F.R.;
RT "Molecular evolution of a pathogenicity island from enterohemorrhagic
RT Escherichia coli O157:H7.";
RL Infect. Immun. 66:3810-3817(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX PubMed=11206551; DOI=10.1038/35054089;
RA Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA Blattner F.R.;
RT "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL Nature 409:529-533(2001).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA Shiba T., Hattori M., Shinagawa H.;
RT "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT genomic comparison with a laboratory strain K-12.";
RL DNA Res. 8:11-22(2001).
RN [5]
RP X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS), AND MUTAGENESIS.
RX PubMed=11685226; DOI=10.1038/nsb717;
RA Luo Y., Bertero M.G., Frey E.A., Pfuetzner R.A., Wenk M.R., Creagh L.,
RA Marcus S.L., Lim D., Sicheri F., Kay C., Haynes C., Finlay B.B.,
RA Strynadka N.C.J.;
RT "Structural and biochemical characterization of the type III secretion
RT chaperones CesT and SigE.";
RL Nat. Struct. Biol. 8:1031-1036(2001).
CC -!- FUNCTION: Chaperone for the type III secretion of Tir. Probably
CC stabilizes the protein, prevents inappropriate protein-
CC proteininteractions and aids in secretion (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: Homodimer.
CC -!- INTERACTION:
CC P58233; Q7DB77: tir; NbExp=2; IntAct=EBI-6403832, EBI-6480811;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the CesT/SycH chaperone family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; U32312; AAB00110.1; -; Genomic_DNA.
DR EMBL; AF071034; AAC31505.1; -; Genomic_DNA.
DR EMBL; AE005174; AAG58824.1; -; Genomic_DNA.
DR EMBL; BA000007; BAB37983.1; -; Genomic_DNA.
DR PIR; D86045; D86045.
DR PIR; H91198; H91198.
DR RefSeq; NP_312587.1; NC_002695.1.
DR RefSeq; WP_000098793.1; NZ_SWKA01000005.1.
DR PDB; 1K3E; X-ray; 2.80 A; A/B=1-156.
DR PDBsum; 1K3E; -.
DR AlphaFoldDB; P58233; -.
DR SMR; P58233; -.
DR IntAct; P58233; 1.
DR STRING; 155864.EDL933_4948; -.
DR EnsemblBacteria; AAG58824; AAG58824; Z5111.
DR EnsemblBacteria; BAB37983; BAB37983; ECs_4560.
DR GeneID; 915463; -.
DR KEGG; ece:Z5111; -.
DR KEGG; ecs:ECs_4560; -.
DR PATRIC; fig|386585.9.peg.4777; -.
DR eggNOG; ENOG5032R50; Bacteria.
DR HOGENOM; CLU_1683457_0_0_6; -.
DR OMA; TNDEYMM; -.
DR EvolutionaryTrace; P58233; -.
DR Proteomes; UP000000558; Chromosome.
DR Proteomes; UP000002519; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0030254; P:protein secretion by the type III secretion system; IEA:InterPro.
DR InterPro; IPR010261; Tir_chaperone.
DR Pfam; PF05932; CesT; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Chaperone; Cytoplasm; Reference proteome; Virulence.
FT CHAIN 1..156
FT /note="Tir chaperone"
FT /id="PRO_0000216362"
FT MUTAGEN 67
FT /note="L->E: Abolishes Tir binding."
FT /evidence="ECO:0000269|PubMed:11685226"
FT MUTAGEN 70
FT /note="L->E: Abolishes Tir binding."
FT /evidence="ECO:0000269|PubMed:11685226"
FT MUTAGEN 74
FT /note="L->E: Abolishes Tir binding."
FT /evidence="ECO:0000269|PubMed:11685226"
FT MUTAGEN 75
FT /note="W->E: Abolishes Tir binding."
FT /evidence="ECO:0000269|PubMed:11685226"
FT MUTAGEN 83
FT /note="Y->E: Abolishes Tir binding."
FT /evidence="ECO:0000269|PubMed:11685226"
FT MUTAGEN 126
FT /note="V->E: Abolishes Tir binding."
FT /evidence="ECO:0000269|PubMed:11685226"
FT HELIX 4..16
FT /evidence="ECO:0007829|PDB:1K3E"
FT STRAND 30..33
FT /evidence="ECO:0007829|PDB:1K3E"
FT STRAND 38..42
FT /evidence="ECO:0007829|PDB:1K3E"
FT STRAND 45..57
FT /evidence="ECO:0007829|PDB:1K3E"
FT HELIX 63..78
FT /evidence="ECO:0007829|PDB:1K3E"
FT STRAND 83..87
FT /evidence="ECO:0007829|PDB:1K3E"
FT TURN 88..91
FT /evidence="ECO:0007829|PDB:1K3E"
FT STRAND 92..100
FT /evidence="ECO:0007829|PDB:1K3E"
FT HELIX 106..128
FT /evidence="ECO:0007829|PDB:1K3E"
FT TURN 129..131
FT /evidence="ECO:0007829|PDB:1K3E"
FT STRAND 141..144
FT /evidence="ECO:0007829|PDB:1K3E"
SQ SEQUENCE 156 AA; 17681 MW; 999545426E26D2D6 CRC64;
MSSRSELLLE KFAEKIGIGS ISFNENRLCS FAIDEIYYIS LSDANDEYMM IYGVCGKFPT
DNSNFALEIL NANLWFAENG GPYLCYEAGA QSLLLALRFP LDDATPEKLE NEIEVVVKSM
ENLYLVLHNQ GITLENEHMK IEEISSSDNK HYYAGR
