ID   CEST_ECOLX              Reviewed;         156 AA.
AC   Q47015; Q47017;
DT   14-AUG-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 86.
DE   RecName: Full=Tir chaperone;
GN   Name=cesT;
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=84/110/1, and RDEC-1;
RA   Krejany E.O.;
RL   Submitted (MAY-1996) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=O15:H- / 83/39 / ETEC;
RA   O'Brien R.A.;
RL   Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=O26:H- / 413/89-1 / EHEC;
RA   Deibel C., Kraemer S., Chakraborty T., Ebel F.;
RL   Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=RDEC-1;
RX   PubMed=8900070; DOI=10.1111/j.1574-6968.1996.tb08538.x;
RA   Agin T.S., Cantey J.R., Boedeker E.C., Wolf M.K.;
RT   "Characterization of the eaeA gene from rabbit enteropathogenic Escherichia
RT   coli strain RDEC-1 and comparison to other eaeA genes from bacteria that
RT   cause attaching-effacing lesions.";
RL   FEMS Microbiol. Lett. 144:249-258(1996).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=RDEC-1;
RX   PubMed=11254564; DOI=10.1128/iai.69.4.2107-2115.2001;
RA   Zhu C., Agin T.S., Elliott S.J., Johnson L.A., Thate T.E., Kaper J.B.,
RA   Boedeker E.C.;
RT   "Complete nucleotide sequence and analysis of the locus of enterocyte
RT   effacement from rabbit diarrheagenic Escherichia coli RDEC-1.";
RL   Infect. Immun. 69:2107-2115(2001).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=O103:H2 / B10 / EPEC;
RX   PubMed=10722617; DOI=10.1128/iai.68.4.2171-2182.2000;
RA   Marches O., Nougayrede J.-P., Boullier S., Mainil J., Charlier G.,
RA   Raymond I., Pohl P., Boury M., De Rycke J., Milon A., Oswald E.;
RT   "Role of tir and intimin in the virulence of rabbit enteropathogenic
RT   Escherichia coli serotype O103:H2.";
RL   Infect. Immun. 68:2171-2182(2000).
RN   [7]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=CK379;
RA   Cid D.;
RT   "Association between intimin (eae) and EspB (espB) gene subtypes in
RT   Attaching and effacing Escherichia coli strains isolated from diarrheic
RT   lambs and goat kids.";
RL   Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Chaperone for the type III secretion of Tir. Probably
CC       stabilizes the protein, prevents inappropriate protein-protein
CC       interactions and aids in secretion (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the CesT/SycH chaperone family. {ECO:0000305}.
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DR   EMBL; U59502; AAC32029.1; -; Genomic_DNA.
DR   EMBL; U59503; AAB02942.1; -; Genomic_DNA.
DR   EMBL; U59504; AAB39867.1; -; Genomic_DNA.
DR   EMBL; U60002; AAB36673.1; -; Genomic_DNA.
DR   EMBL; AJ223063; CAA11066.1; -; Genomic_DNA.
DR   EMBL; AF200363; AAK26723.1; -; Genomic_DNA.
DR   EMBL; AF113597; AAF03081.1; -; Genomic_DNA.
DR   EMBL; AF253560; AAK48431.1; -; Genomic_DNA.
DR   PDB; 5Z38; X-ray; 2.29 A; A/B/C/D=1-156.
DR   PDBsum; 5Z38; -.
DR   AlphaFoldDB; Q47015; -.
DR   BMRB; Q47015; -.
DR   SMR; Q47015; -.
DR   DIP; DIP-27658N; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0030254; P:protein secretion by the type III secretion system; IEA:InterPro.
DR   InterPro; IPR010261; Tir_chaperone.
DR   Pfam; PF05932; CesT; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Chaperone; Cytoplasm; Virulence.
FT   CHAIN           1..156
FT                   /note="Tir chaperone"
FT                   /id="PRO_0000216363"
FT   VARIANT         151
FT                   /note="Y -> H (in strain: RDEC-1, 83/39, 413/89-1 and
FT                   CK379)"
FT   HELIX           4..16
FT                   /evidence="ECO:0007829|PDB:5Z38"
FT   STRAND          27..33
FT                   /evidence="ECO:0007829|PDB:5Z38"
FT   TURN            34..36
FT                   /evidence="ECO:0007829|PDB:5Z38"
FT   STRAND          37..42
FT                   /evidence="ECO:0007829|PDB:5Z38"
FT   STRAND          46..57
FT                   /evidence="ECO:0007829|PDB:5Z38"
FT   HELIX           63..78
FT                   /evidence="ECO:0007829|PDB:5Z38"
FT   STRAND          83..87
FT                   /evidence="ECO:0007829|PDB:5Z38"
FT   TURN            88..91
FT                   /evidence="ECO:0007829|PDB:5Z38"
FT   STRAND          92..100
FT                   /evidence="ECO:0007829|PDB:5Z38"
FT   HELIX           106..129
FT                   /evidence="ECO:0007829|PDB:5Z38"
FT   HELIX           148..151
FT                   /evidence="ECO:0007829|PDB:5Z38"
SQ   SEQUENCE   156 AA;  17747 MW;  08F3A01D716C41DF CRC64;
     MSSRSELLLD RFAEKIGIGS ISFNENRLCS FAIDEIYYIS LSDANDEYMM IYGVCGKFPT
     DNPNFALEIL NANLWFAENG GPYLCYESGA QSLLLALRFP LDDATPEKLE NEIEVVVKSM
     ENLYLVLHNQ GITLENEHMK IEEISSSDNK YYYAGR