ID   CEUL_OPHUL              Reviewed;         100 AA.
AC   Q06153;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   25-MAY-2022, entry version 71.
DE   RecName: Full=Cerato-ulmin;
DE            Short=CU;
DE   AltName: Full=Dutch elm disease toxin;
DE   Flags: Precursor;
GN   Name=CU;
OS   Ophiostoma ulmi (Dutch elm disease fungus).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Ophiostomatales; Ophiostomataceae; Ophiostoma.
OX   NCBI_TaxID=5174;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=MH75;
RX   PubMed=8082175; DOI=10.1007/bf00351485;
RA   Bowden C.G., Hintz W.E., Jeng R., Hubbes M., Horgen P.A.;
RT   "Isolation and characterization of the cerato-ulmin toxin gene of the Dutch
RT   elm disease pathogen, Ophiostoma ulmi.";
RL   Curr. Genet. 25:323-329(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=P1;
RX   PubMed=8821664; DOI=10.1007/bf02221581;
RA   Jeng R., Hintz W.E., Bowden C.G., Horgen P.A., Hubbes M.;
RT   "A comparison of the nucleotide sequence of the cerato-ulmin gene and the
RT   rDNA ITS between aggressive and non-aggressive isolates of Ophiostoma ulmi
RT   sensu lato, the causal agent of Dutch elm disease.";
RL   Curr. Genet. 29:168-173(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE OF 26-100.
RX   PubMed=1477406; DOI=10.1094/mpmi-5-520;
RA   Bolyard M.G., Sticklen M.B.;
RT   "Expression of a modified Dutch elm disease toxin in Escherichia coli.";
RL   Mol. Plant Microbe Interact. 5:520-524(1992).
RN   [4]
RP   PROTEIN SEQUENCE OF 26-100.
RA   Yaguchi M., Pusztai-Carey M., Roy C., Surewicz W.K., Carey P.R.,
RA   Stevenson K.J., Richards W.C., Takai S.;
RL   (In) Stricklen M., Sherald J. (eds.);
RL   Dutch elm disease research: cellular and molecular approaches, pp.152-170,
RL   Springer-Verlag, New York (1993).
CC   -!- FUNCTION: Has been implicated in the pathogenicity of this fungus on
CC       ELM. Accumulates at, and plugs intercellular openings in the xylem, or
CC       interacts with host parenchyma cells, thereby enhancing respiration and
CC       electrolyte loss.
CC   -!- SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000250}.
CC   -!- MISCELLANEOUS: Strain P1 is a non-aggressive isolate, while strain MH75
CC       is an aggressive isolate.
CC   -!- SIMILARITY: Belongs to the cerato-ulmin hydrophobin family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; U00963; AAA18912.1; -; Unassigned_DNA.
DR   EMBL; U23203; AAB41284.1; -; Genomic_DNA.
DR   EMBL; S52233; AAB24789.1; -; Genomic_DNA.
DR   PIR; S41996; A44596.
DR   PIR; S62753; S62753.
DR   AlphaFoldDB; Q06153; -.
DR   SMR; Q06153; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.20.120.10; -; 1.
DR   InterPro; IPR010636; Cerato-ulmin_hydrophobin.
DR   InterPro; IPR036686; Hydrophobin_sf.
DR   PANTHER; PTHR42341; PTHR42341; 1.
DR   Pfam; PF06766; Hydrophobin_2; 1.
DR   SUPFAM; SSF101751; SSF101751; 1.
PE   1: Evidence at protein level;
KW   Cell wall; Direct protein sequencing; Secreted; Signal; Toxin; Virulence.
FT   SIGNAL          1..25
FT                   /evidence="ECO:0000269|Ref.4"
FT   CHAIN           26..100
FT                   /note="Cerato-ulmin"
FT                   /id="PRO_0000013517"
FT   VARIANT         16
FT                   /note="M -> L (in strain: P1)"
FT   VARIANT         23
FT                   /note="N -> S (in strain: P1)"
FT   VARIANT         28
FT                   /note="S -> P (in strain: P1)"
FT   VARIANT         74
FT                   /note="A -> R (in strain: P1)"
FT   VARIANT         79
FT                   /note="S -> Q (in strain: P1)"
SQ   SEQUENCE   100 AA;  10202 MW;  E214D6CF600A5BF1 CRC64;
     MQFSIATIAL FLSSAMAAPY SGNSNSDSYD PCTGLLQKSP QCCNTDILGV ANLDCHGPPS
     VPTSPSQFQA SCVADGGRSA RCCTLSLLGL ALVCTDPVGI