CEX1_ASPNA
ID CEX1_ASPNA Reviewed; 524 AA.
AC G3Y4N5;
DT 10-FEB-2021, integrated into UniProtKB/Swiss-Prot.
DT 10-FEB-2021, sequence version 2.
DT 25-MAY-2022, entry version 32.
DE RecName: Full=Citrate exporter 1 {ECO:0000303|PubMed:30553933};
GN Name=cex1 {ECO:0000305}; Synonyms=cexA {ECO:0000303|PubMed:30553933};
GN ORFNames=ASPNIDRAFT2_1165828 {ECO:0000305},
GN ASPNIDRAFT_57285 {ECO:0000312|EMBL:EHA22412.1};
OS Aspergillus niger (strain ATCC 1015 / CBS 113.46 / FGSC A1144 / LSHB Ac4 /
OS NCTC 3858a / NRRL 328 / USDA 3528.7).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Circumdati.
OX NCBI_TaxID=380704 {ECO:0000312|Proteomes:UP000009038};
RN [1] {ECO:0000312|Proteomes:UP000009038}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 1015 / CBS 113.46 / FGSC A1144 / LSHB Ac4 / NCTC 3858a / NRRL
RC 328 / USDA 3528.7 {ECO:0000312|Proteomes:UP000009038};
RX PubMed=21543515; DOI=10.1101/gr.112169.110;
RA Andersen M.R., Salazar M.P., Schaap P.J., van de Vondervoort P.J.I.,
RA Culley D., Thykaer J., Frisvad J.C., Nielsen K.F., Albang R., Albermann K.,
RA Berka R.M., Braus G.H., Braus-Stromeyer S.A., Corrochano L.M., Dai Z.,
RA van Dijck P.W.M., Hofmann G., Lasure L.L., Magnuson J.K., Menke H.,
RA Meijer M., Meijer S.L., Nielsen J.B., Nielsen M.L., van Ooyen A.J.J.,
RA Pel H.J., Poulsen L., Samson R.A., Stam H., Tsang A., van den Brink J.M.,
RA Atkins A., Aerts A., Shapiro H., Pangilinan J., Salamov A., Lou Y.,
RA Lindquist E., Lucas S., Grimwood J., Grigoriev I.V., Kubicek C.P.,
RA Martinez D., van Peij N.N.M.E., Roubos J.A., Nielsen J., Baker S.E.;
RT "Comparative genomics of citric-acid-producing Aspergillus niger ATCC 1015
RT versus enzyme-producing CBS 513.88.";
RL Genome Res. 21:885-897(2011).
RN [2] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 1015 / CBS 113.46 / FGSC A1144 / LSHB Ac4 / NCTC 3858a / NRRL
RC 328 / USDA 3528.7 {ECO:0000303|PubMed:30553933};
RX PubMed=30553933; DOI=10.1016/j.ymben.2018.12.004;
RA Steiger M.G., Rassinger A., Mattanovich D., Sauer M.;
RT "Engineering of the citrate exporter protein enables high citric acid
RT production in Aspergillus niger.";
RL Metab. Eng. 52:224-231(2019).
RN [3] {ECO:0000305}
RP FUNCTION, AND SUBCELLULAR LOCATION.
RC STRAIN=ATCC 1015 / CBS 113.46 / FGSC A1144 / LSHB Ac4 / NCTC 3858a / NRRL
RC 328 / USDA 3528.7 {ECO:0000303|PubMed:32990722};
RX PubMed=32990722; DOI=10.1093/femsyr/foaa055;
RA Erian A.M., Egermeier M., Rassinger A., Marx H., Sauer M.;
RT "Identification of the citrate exporter Cex1 of Yarrowia lipolytica.";
RL FEMS Yeast Res. 0:0-0(2020).
CC -!- FUNCTION: Transmembrane transporter that exports citrate across the
CC cell membrane. {ECO:0000269|PubMed:30553933,
CC ECO:0000269|PubMed:32990722}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=citrate(in) = citrate(out); Xref=Rhea:RHEA:33183,
CC ChEBI:CHEBI:16947; Evidence={ECO:0000305|PubMed:30553933,
CC ECO:0000305|PubMed:32990722};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305|PubMed:30553933,
CC ECO:0000305|PubMed:32990722}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- DISRUPTION PHENOTYPE: Decreases concentration of citric acid and
CC increases concentration of oxalic acid in growth medium.
CC {ECO:0000269|PubMed:30553933}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=EHA22412.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; ACJE01000012; EHA22412.1; ALT_SEQ; Genomic_DNA.
DR AlphaFoldDB; G3Y4N5; -.
DR STRING; 380704.G3Y4N5; -.
DR EnsemblFungi; EHA22412; EHA22412; ASPNIDRAFT_57285.
DR HOGENOM; CLU_008455_8_4_1; -.
DR Proteomes; UP000009038; Unassembled WGS sequence.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IC:UniProtKB.
DR GO; GO:0015137; F:citrate transmembrane transporter activity; IMP:UniProtKB.
DR GO; GO:0015746; P:citrate transport; IMP:UniProtKB.
DR GO; GO:0140115; P:export across plasma membrane; IMP:UniProtKB.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 3: Inferred from homology;
KW Cell membrane; Glycoprotein; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..524
FT /note="Citrate exporter 1"
FT /id="PRO_0000452022"
FT TRANSMEM 60..80
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 95..115
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 125..145
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 155..175
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 186..206
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 215..235
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 296..316
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 332..352
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 395..415
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 417..437
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 459..479
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 481..501
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..49
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 24..44
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 90
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 244
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 524 AA; 56776 MW; 26618A9C4220C61D CRC64;
MSSTTSSSRS DLEKVPVPQV TPRDSDSDKG SLSPEPSTLE AQSSEKPPHH IFTRSRKLQM
VCIVSLAAIF SPLSSNIYFP ALDDVSKSLN ISMSLATLTI TVYMIVQGLA PSFWGSMSDA
TGRRPVFIGT FIVYLVANIA LAESKNYGEL MAFRALQAAG SAATISIGAG VIGDITNSEE
RGSLVGIFGG VRMLGQGIGP VFGGIFTQYL GYRSIFWFLT IAGGVSLLSI LVLLPETLRP
IAGNGTVKLN GIHKPFIYTI TGQTGVVEGA QPEAKKTKTS WKSVFAPLTF LVEKDVFITL
FFGSIVYTVW SMVTSSTTDL FSEVYGLSSL DIGLTFLGNG FGCMSGSYLV GYLMDYNHRL
TEREYCEKHG YPAGTRVNLK SHPDFPIEVA RMRNTWWVIA IFIVTVALYG VSLRTHLAVP
IILQYFIAFC STGLFTINSA LVIDLYPGAS ASATAVNNLM RCLLGAGGVA IVQPILDALK
PDYTFLLLAG ITLVMTPLLY VEDRWGPGWR HARERRLKAK ANGN
