CF130_YEAST
ID CF130_YEAST Reviewed; 1122 AA.
AC P53280; D6VUR6;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 157.
DE RecName: Full=Protein CAF130;
DE AltName: Full=130 kDa CCR4-associated factor;
GN Name=CAF130; OrderedLocusNames=YGR134W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169869;
RA Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J.,
RA Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M.,
RA Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L.,
RA Coblenz A., Coglievina M., Coissac E., Defoor E., Del Bino S., Delius H.,
RA Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P.,
RA Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M.,
RA Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A.,
RA Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K.,
RA Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P.,
RA Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E.,
RA Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K.,
RA Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A.,
RA Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S.,
RA Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M.,
RA Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C.,
RA Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M.,
RA Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M.,
RA Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y.,
RA Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L.,
RA Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D.,
RA Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F.,
RA Zaccaria P., Zimmermann M., Zollner A., Kleine K.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.";
RL Nature 387:81-84(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP IDENTIFICATION IN THE CCR4-NOT CORE COMPLEX, AND INTERACTION WITH NOT1.
RX PubMed=11733989; DOI=10.1006/jmbi.2001.5162;
RA Chen J., Rappsilber J., Chiang Y.C., Russell P., Mann M., Denis C.L.;
RT "Purification and characterization of the 1.0 MDa CCR4-NOT complex
RT identifies two novel components of the complex.";
RL J. Mol. Biol. 314:683-694(2001).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1042, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- FUNCTION: Acts as component of the CCR4-NOT core complex, which in the
CC nucleus seems to be a general transcription factor, and in the
CC cytoplasm the major mRNA deadenylase involved in mRNA turnover.
CC -!- SUBUNIT: Subunit of the 1.0 MDa CCR4-NOT core complex that contains
CC CCR4, CAF1, NOT1, NOT2, NOT3, NOT4, NOT5, CAF40 and CAF130. In the
CC complex interacts with NOT1. The core complex probably is part of a
CC less characterized 1.9 MDa CCR4-NOT complex.
CC {ECO:0000269|PubMed:11733989}.
CC -!- INTERACTION:
CC P53280; P29366: BEM1; NbExp=3; IntAct=EBI-23322, EBI-3508;
CC P53280; P25655: CDC39; NbExp=6; IntAct=EBI-23322, EBI-12139;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}. Nucleus {ECO:0000305}.
CC -!- MISCELLANEOUS: Present with 1320 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; Z72919; CAA97147.1; -; Genomic_DNA.
DR EMBL; AY558112; AAS56438.1; -; Genomic_DNA.
DR EMBL; BK006941; DAA08227.1; -; Genomic_DNA.
DR PIR; S64443; S64443.
DR RefSeq; NP_011650.3; NM_001181263.3.
DR AlphaFoldDB; P53280; -.
DR BioGRID; 33382; 158.
DR ComplexPortal; CPX-1800; CCR4-NOT mRNA deadenylase complex.
DR DIP; DIP-2683N; -.
DR IntAct; P53280; 9.
DR MINT; P53280; -.
DR STRING; 4932.YGR134W; -.
DR iPTMnet; P53280; -.
DR MaxQB; P53280; -.
DR PaxDb; P53280; -.
DR PRIDE; P53280; -.
DR TopDownProteomics; P53280; -.
DR ABCD; P53280; 7 sequenced antibodies.
DR EnsemblFungi; YGR134W_mRNA; YGR134W; YGR134W.
DR GeneID; 853035; -.
DR KEGG; sce:YGR134W; -.
DR SGD; S000003366; CAF130.
DR VEuPathDB; FungiDB:YGR134W; -.
DR eggNOG; ENOG502QT7N; Eukaryota.
DR HOGENOM; CLU_280757_0_0_1; -.
DR InParanoid; P53280; -.
DR OMA; EMLMCSG; -.
DR BioCyc; YEAST:G3O-30840-MON; -.
DR PRO; PR:P53280; -.
DR Proteomes; UP000002311; Chromosome VII.
DR RNAct; P53280; protein.
DR GO; GO:0030015; C:CCR4-NOT core complex; IPI:SGD.
DR GO; GO:0005829; C:cytosol; HDA:SGD.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000289; P:nuclear-transcribed mRNA poly(A) tail shortening; IC:ComplexPortal.
DR GO; GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; IDA:SGD.
PE 1: Evidence at protein level;
KW Activator; Cytoplasm; Nucleus; Phosphoprotein; Reference proteome;
KW Repressor; Transcription; Transcription regulation.
FT CHAIN 1..1122
FT /note="Protein CAF130"
FT /id="PRO_0000202824"
FT REGION 1..24
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 7..22
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 1042
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18407956"
SQ SEQUENCE 1122 AA; 128780 MW; BF3542816CC78490 CRC64;
MTKKKAATNY AERQNLASED SSGDSVHFKD FIPLQELLKD KNYVPSVENL EKILYNETMF
NDQKICSNLL LEALIITLFT TISGKSALRL IQTSSLKERK SWAQSFENNS SSYASIVLSW
KDNDILLLKF LRFLLANKTA PLQINRYNLP EYKLPLSFLI VSKITIPSIL LNETYNLLKD
YLYSITGRIE SLISCSSTFD KPALVVRKIL KDYNRMIECR NFYFWYSFNA ENRVNLTFSD
NISLLMENDE GNAGSGLDDS RFDHQKQPRE AIMGRTINDQ EQIYSFELNQ DGTLEIPNVM
EHSLLRHELL FKILNLTTVL TPLLELQFST LCGLVDPLMQ PTPNDKHIIS IDFLFQLFLG
LMSQSIKTSQ EHNDHYDWKF YMCFNMQKII DATMLRLNCF DFDILNSVNN TDNAVHWKTQ
LHRWLPHGLN TQDLELLYMI DILAVYTIYK LYEKIPIQLN PFLFSLISLW KNLSCVILLA
LEIDRIEEEN GTYETPLMVR ATIRGAAALR SVIATVLNGL VKNNDHDFKH ESLNTFMSPY
GRKLCHGALY ADLRSHTASL LALGASIEDV TDLFADLQSG DRFDEDIRYM FDYECEDYDE
SFSESDHGGL DESVVNPTEK IASGSNNVFF RRRCNCIFND DKLVAEDGAN EAFGSTNSEN
VEGAMHNNRN AVHNATTATS DHVVTSPNPL SVRSRSTFEF DYSGEDWRDV PRDFNMYYSP
SYSFIHEPKL DVIFSLTLRG ATEKLNKEES ILLVRSVASC VRNEQDQMIL ADLESNFSAS
INGDVEGEGN TKMSKIDNED LRRTTPDDIY EIWSEESAFE RMLNVNHDVA WRLMDEMLMC
TGYRRILIWF LTHLELKHSL IYYVFELIMG LRGKPFSGEA SDQDKKDDMI YEILKKKQKN
EDASGLPFSR QGPIVLSDIE TKMLLQEFFM NAAIFLSSKN NEEENEDGEK ISLYSLGLVR
LICYMVQTLI ANDKFFFTKS ECTFELQTLL MTWIGILPEA KDLFFKIKTR LAMEEEDSAD
TMQHEGRKNS DIEKKLNAKP ASELNLKLLN LFPSKPANKD DSSPINTLRS FIADYSFDTQ
VNPPGRRVVF YDGKILPLPK ADKPIPLHEY ITLAELDVGD SE
