CF251_CHLRE
ID CF251_CHLRE Reviewed; 815 AA.
AC A8IRK7;
DT 12-APR-2017, integrated into UniProtKB/Swiss-Prot.
DT 04-DEC-2007, sequence version 1.
DT 03-AUG-2022, entry version 65.
DE RecName: Full=Cilia- and flagella-associated protein 251 {ECO:0000305};
DE AltName: Full=Flagellar-associated protein 251 {ECO:0000305};
GN Name=CFAP251 {ECO:0000305}; Synonyms=FAP251 {ECO:0000303|PubMed:17967944};
GN ORFNames=CHLREDRAFT_145396 {ECO:0000312|EMBL:EDP04324.1};
OS Chlamydomonas reinhardtii (Chlamydomonas smithii).
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX NCBI_TaxID=3055;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CC-503;
RX PubMed=17932292; DOI=10.1126/science.1143609;
RA Merchant S.S., Prochnik S.E., Vallon O., Harris E.H., Karpowicz S.J.,
RA Witman G.B., Terry A., Salamov A., Fritz-Laylin L.K., Marechal-Drouard L.,
RA Marshall W.F., Qu L.H., Nelson D.R., Sanderfoot A.A., Spalding M.H.,
RA Kapitonov V.V., Ren Q., Ferris P., Lindquist E., Shapiro H., Lucas S.M.,
RA Grimwood J., Schmutz J., Cardol P., Cerutti H., Chanfreau G., Chen C.L.,
RA Cognat V., Croft M.T., Dent R., Dutcher S., Fernandez E., Fukuzawa H.,
RA Gonzalez-Ballester D., Gonzalez-Halphen D., Hallmann A., Hanikenne M.,
RA Hippler M., Inwood W., Jabbari K., Kalanon M., Kuras R., Lefebvre P.A.,
RA Lemaire S.D., Lobanov A.V., Lohr M., Manuell A., Meier I., Mets L.,
RA Mittag M., Mittelmeier T., Moroney J.V., Moseley J., Napoli C.,
RA Nedelcu A.M., Niyogi K., Novoselov S.V., Paulsen I.T., Pazour G.J.,
RA Purton S., Ral J.P., Riano-Pachon D.M., Riekhof W., Rymarquis L.,
RA Schroda M., Stern D., Umen J., Willows R., Wilson N., Zimmer S.L.,
RA Allmer J., Balk J., Bisova K., Chen C.J., Elias M., Gendler K., Hauser C.,
RA Lamb M.R., Ledford H., Long J.C., Minagawa J., Page M.D., Pan J.,
RA Pootakham W., Roje S., Rose A., Stahlberg E., Terauchi A.M., Yang P.,
RA Ball S., Bowler C., Dieckmann C.L., Gladyshev V.N., Green P., Jorgensen R.,
RA Mayfield S., Mueller-Roeber B., Rajamani S., Sayre R.T., Brokstein P.,
RA Dubchak I., Goodstein D., Hornick L., Huang Y.W., Jhaveri J., Luo Y.,
RA Martinez D., Ngau W.C., Otillar B., Poliakov A., Porter A., Szajkowski L.,
RA Werner G., Zhou K., Grigoriev I.V., Rokhsar D.S., Grossman A.R.;
RT "The Chlamydomonas genome reveals the evolution of key animal and plant
RT functions.";
RL Science 318:245-250(2007).
RN [2]
RP FUNCTION, IDENTIFICATION IN A COMPLEX CONTAINING CFAP61; CFAP91 AND
RP CFAP251, SUBCELLULAR LOCATION, AND INTERACTION WITH CALMODULIN.
RX PubMed=17967944; DOI=10.1083/jcb.200703107;
RA Dymek E.E., Smith E.F.;
RT "A conserved CaM- and radial spoke associated complex mediates regulation
RT of flagellar dynein activity.";
RL J. Cell Biol. 179:515-526(2007).
CC -!- FUNCTION: As component of a spoke-associated complex, regulates
CC flagellar dynein activity by mediating regulatory signals between the
CC radial spokes and dynein arms. {ECO:0000269|PubMed:17967944}.
CC -!- SUBUNIT: Identified in a spoke-associated complex containing CFAP61,
CC CFAP91 and CFAP251; the complex is associated with the radial spokes in
CC the axoneme (PubMed:17967944). The complex associates with Calmodulin;
CC the association is calcium sensitive (PubMed:17967944).
CC {ECO:0000269|PubMed:17967944}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, flagellum axoneme
CC {ECO:0000269|PubMed:17967944}.
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DR EMBL; DS496121; EDP04324.1; -; Genomic_DNA.
DR RefSeq; XP_001691834.1; XM_001691782.1.
DR AlphaFoldDB; A8IRK7; -.
DR STRING; 3055.EDP04324; -.
DR PaxDb; A8IRK7; -.
DR PRIDE; A8IRK7; -.
DR EnsemblPlants; PNW85421; PNW85421; CHLRE_03g185650v5.
DR GeneID; 5717442; -.
DR Gramene; PNW85421; PNW85421; CHLRE_03g185650v5.
DR eggNOG; ENOG502QQ05; Eukaryota.
DR HOGENOM; CLU_007087_1_0_1; -.
DR InParanoid; A8IRK7; -.
DR OrthoDB; 169738at2759; -.
DR GO; GO:0005930; C:axoneme; IDA:UniProtKB.
DR GO; GO:0031514; C:motile cilium; IDA:UniProtKB.
DR GO; GO:0003341; P:cilium movement; IDA:UniProtKB.
DR Gene3D; 2.130.10.10; -; 2.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR SMART; SM00320; WD40; 9.
DR SUPFAM; SSF50978; SSF50978; 1.
PE 1: Evidence at protein level;
KW Cell projection; Cilium; Cytoplasm; Cytoskeleton; Flagellum; Repeat;
KW WD repeat.
FT CHAIN 1..815
FT /note="Cilia- and flagella-associated protein 251"
FT /id="PRO_0000439533"
FT REPEAT 58..99
FT /note="WD 1"
FT /evidence="ECO:0000255"
FT REPEAT 103..148
FT /note="WD 2"
FT /evidence="ECO:0000255"
FT REPEAT 166..205
FT /note="WD 3"
FT /evidence="ECO:0000255"
FT REPEAT 218..257
FT /note="WD 4"
FT /evidence="ECO:0000255"
FT REPEAT 271..308
FT /note="WD 5"
FT /evidence="ECO:0000255"
FT REPEAT 379..418
FT /note="WD 6"
FT /evidence="ECO:0000255"
FT REPEAT 420..460
FT /note="WD 7"
FT /evidence="ECO:0000255"
FT REPEAT 463..502
FT /note="WD 8"
FT /evidence="ECO:0000255"
FT REPEAT 511..553
FT /note="WD 9"
FT /evidence="ECO:0000255"
FT REPEAT 573..612
FT /note="WD 10"
FT /evidence="ECO:0000255"
SQ SEQUENCE 815 AA; 87630 MW; AAB6DC4839007F83 CRC64;
MAEEQTALSL SWVFGASAHV KHGVVNLSDG YTDKICYLAA NTAVIYDKRL RRQLFLQGHT
SPITCIVTTE DRSHVVTADT GPEALLVVWN VRTGLPTRTV QQPHRHGVST MDMSADGQWL
ATVSAADPES GEQEVSLWSM AALLTPPEAA PPGQGPLRPL VTTLVPAGDV QHSIRFSPNN
PAELISNGRR RVYFWSWAPG SPRFQYYSPP LRSRDFKQSV GDFVSSVFVP GTTQALTATT
DGDLVVWDEQ GIAAQVGTSA TDRRAIKLMR IHNCPITLLA TVGDFIVSGG EDGYVRFFDP
LLRIVAWFED LAAGPVTSVA FSAVLPDRLA HADAADTLNR FMVPDFVVAT RNSRIVSVQS
ASFEEYDADR RRGSSVLDSL LADVVDLAAH PTRAEFAVLG RDGGLQRWDS IAHCLLGGRA
FERQVGACLT YSRDGSLLVV GFGSGHLHIL NADDCSDLYV MRNTAAGLVR VAVSNTGKHI
AAADENHQLL LYAYLPYKHT MRWEYVGRCR SHHGPIASVV FGESPSGQTR LLSVGGDGRV
VEYDLAASSV AAGVQVASFY DFPPGGGAPT SLSFAPPLAY FQAFAADTHL LVSGADGTVA
SWDINTAPLE RSATAAEGAG GEARWAAVLG DPDLLREMRD YFVYAQIKTQ GEDALEPRDV
PGTVPVDLVP DLMRSAGFYP SESDIDNLLH HVQYMAHSRN MESLEVVTLA DLLCLYINHR
PLFNVTHADI VAAFRELGGR GDPGSCVPKL SREQLLSLLQ STGEPMSGEE LTAALAALTG
AHTPEKSMPV SVAAEQFSAD VLGFDTTEAG AEAAT
