CF251_MOUSE
ID CF251_MOUSE Reviewed; 1299 AA.
AC E9Q743; Q8BMR0; Q9D3X6;
DT 12-APR-2017, integrated into UniProtKB/Swiss-Prot.
DT 05-APR-2011, sequence version 1.
DT 03-AUG-2022, entry version 68.
DE RecName: Full=Cilia- and flagella-associated protein 251 {ECO:0000305};
DE AltName: Full=WD repeat-containing protein 66 {ECO:0000312|MGI:MGI:1918495};
GN Name=Cfap251 {ECO:0000250|UniProtKB:Q8TBY9};
GN Synonyms=Wdr66 {ECO:0000312|MGI:MGI:1918495};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 483-1299.
RC STRAIN=C57BL/6J;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Involved in spermatozoa motility (By similarity). May also
CC regulate cilium motility through its role in the assembly of the
CC axonemal radial spokes (By similarity). {ECO:0000250|UniProtKB:A8IRK7,
CC ECO:0000250|UniProtKB:Q24DE2, ECO:0000250|UniProtKB:Q8TBY9}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, cilium axoneme
CC {ECO:0000250|UniProtKB:A8IRK7}. Cell projection, cilium, flagellum
CC {ECO:0000250|UniProtKB:Q8TBY9}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB30526.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAC26877.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AC113504; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC163337; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AK030281; BAC26877.1; ALT_INIT; mRNA.
DR EMBL; AK016965; BAB30526.1; ALT_INIT; mRNA.
DR CCDS; CCDS89985.1; -.
DR AlphaFoldDB; E9Q743; -.
DR STRING; 10090.ENSMUSP00000113309; -.
DR iPTMnet; E9Q743; -.
DR PhosphoSitePlus; E9Q743; -.
DR MaxQB; E9Q743; -.
DR PaxDb; E9Q743; -.
DR PRIDE; E9Q743; -.
DR ProteomicsDB; 281202; -.
DR Antibodypedia; 31607; 101 antibodies from 19 providers.
DR Ensembl; ENSMUST00000121964; ENSMUSP00000113309; ENSMUSG00000029442.
DR MGI; MGI:1918495; Wdr66.
DR VEuPathDB; HostDB:ENSMUSG00000029442; -.
DR eggNOG; ENOG502QQ05; Eukaryota.
DR GeneTree; ENSGT00390000013370; -.
DR HOGENOM; CLU_007087_0_0_1; -.
DR InParanoid; E9Q743; -.
DR OMA; ISCCVVS; -.
DR PhylomeDB; E9Q743; -.
DR TreeFam; TF328993; -.
DR ChiTaRS; Wdr66; mouse.
DR PRO; PR:E9Q743; -.
DR Proteomes; UP000000589; Chromosome 5.
DR RNAct; E9Q743; protein.
DR Bgee; ENSMUSG00000029442; Expressed in outer nuclear layer of retina and 102 other tissues.
DR ExpressionAtlas; E9Q743; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0005856; C:cytoskeleton; IEA:UniProtKB-KW.
DR GO; GO:0031514; C:motile cilium; IBA:GO_Central.
DR GO; GO:0036126; C:sperm flagellum; ISS:UniProtKB.
DR GO; GO:0030317; P:flagellated sperm motility; ISS:UniProtKB.
DR Gene3D; 2.130.10.10; -; 2.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR011047; Quinoprotein_ADH-like_supfam.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF00400; WD40; 1.
DR SMART; SM00320; WD40; 8.
DR SUPFAM; SSF47473; SSF47473; 1.
DR SUPFAM; SSF50978; SSF50978; 1.
DR SUPFAM; SSF50998; SSF50998; 1.
PE 1: Evidence at protein level;
KW Cell projection; Cilium; Coiled coil; Cytoplasm; Cytoskeleton; Flagellum;
KW Reference proteome; Repeat; WD repeat.
FT CHAIN 1..1299
FT /note="Cilia- and flagella-associated protein 251"
FT /id="PRO_0000439639"
FT REPEAT 484..526
FT /note="WD 1"
FT /evidence="ECO:0000255"
FT REPEAT 534..574
FT /note="WD 2"
FT /evidence="ECO:0000255"
FT REPEAT 585..624
FT /note="WD 3"
FT /evidence="ECO:0000255"
FT REPEAT 643..678
FT /note="WD 4"
FT /evidence="ECO:0000255"
FT REPEAT 681..741
FT /note="WD 5"
FT /evidence="ECO:0000255"
FT REPEAT 745..785
FT /note="WD 6"
FT /evidence="ECO:0000255"
FT REPEAT 791..828
FT /note="WD 7"
FT /evidence="ECO:0000255"
FT REPEAT 838..874
FT /note="WD 8"
FT /evidence="ECO:0000255"
FT REPEAT 881..924
FT /note="WD 9"
FT /evidence="ECO:0000255"
FT REPEAT 935..975
FT /note="WD 10"
FT /evidence="ECO:0000255"
FT REPEAT 981..1027
FT /note="WD 11"
FT /evidence="ECO:0000255"
FT REPEAT 1033..1071
FT /note="WD 12"
FT /evidence="ECO:0000255"
FT REPEAT 1109..1149
FT /note="WD 13"
FT /evidence="ECO:0000255"
FT REPEAT 1169..1209
FT /note="WD 14"
FT /evidence="ECO:0000255"
FT REGION 1..351
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 12..65
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 73..156
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 159..174
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 187..207
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 208..224
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 225..265
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 280..299
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 309..328
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 330..351
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 922
FT /note="Y -> N (in Ref. 2; BAC26877)"
FT /evidence="ECO:0000305"
FT CONFLICT 1117
FT /note="S -> N (in Ref. 2; BAB30526)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1299 AA; 148608 MW; F4A74246322A101C CRC64;
MSDTEENPLE ESRESGEMEE EKEEKKEEEE EEEEGEKEKE EEEEEEKGKE EEKEEEKEEG
KEEEKKEEEE EKGKKEEEEE EEEEGKGKEE EKEEEKEEEE KEEEKEKEEE EEEEGKGKEE
EEEGKEEEEE EKEEEEKEEE KEEEEEEEEE KEEYNPSFEE DALQQSLTEG SLSWEESQED
GEGLEEWVEK EEQREGEEVR GEEESEVRGR EEEEGWEEEK SGEEEKSEES ERSKERGGEE
EGQEKEEAEH EGEREEGREE EEKEKSVERE EEEEEEDTET TETKAGRAKE EKKEKQNKTA
LVDNIVISHE PPRSSSSLAD SPEVSQIYKD NSMKVDDTEE ASQKPEDILA QGKDEARLSL
EERRKLFQSK GLSAEESLVS VSTEDTLFQK EEDSKVYPLS MTWSFGWNSS LPVYYMREDR
RVILYTCAHT AIMYDVVRNT QYHLQGHPNI ISCLCVSEDR RWIATADEGP DCLIIIWDSF
TGIPVHTIFD SCPEGNGMRS IAITRDSKFL ATISDSATQK VCIWKWTLAV ETPACTLELP
KEYGFQDNLV FNPANNKELV SNSKTQAIYY CWFEDKGILA HSAPVLTEKT FNKLVGKFSQ
SVFHLKLPQV LSATKEGKLV VWDIHYPSST SSSAISAFPF IKPRKLVHLQ KEAITVLMTI
DSYIVTGDIK GNIKFYDHTL SVVNWYSNFK LGAIRTLSFS KTIPSLPTEK SNLPTDCTLR
GDLFVVRNFI IGTFDATVYH MTVDGTKLEK LFVEPRDAVY AVSCHPYQPL IAVGSVCGMI
KVWDFEKKVY LFSRTFEKGL GVQCLTYNPE GALLGAGFTE GTVYILDAMS LENESPEPFK
YSKSSVSHCC FSHDSNYMAT ADVNFTVAVY MVVVKNGQRV WEYLARLRSH QNSIQSLLFG
VHLDSNEPRL LSLGKDRFLI EYNLVKSCKD HLDVLDVHRT DQGNYPTCMI WYPPLTKELF
LLICNSGYKV KLFNATTKMC RKTLLGPAYG SPIEHAQVLP VKSTLELQKR YLVFINKDKV
GLQILPVDGN PHKTCAIVCH PNGVAGMALS YDGRFAFTAG GQDRSVVQWK INLGALEAAV
SLGGEDLTPF YGLVSGGREG KFYRELEDYF YYSQIRSQGI DTMETRQVSE HICLSELPFV
MRAIGFYPSE EKIEDMFNEI KFSEYVETGK LIDKINLPDF LKVYLNHRPP FGNTMDGIQN
SFNVLGYTNS EGKKAIRRED FLNLLLTKGE HMTEEEMIDC FSTLFGLTPE GWKSEPAATC
INGPEICLQK ELPEEITAEI FTTEILGLTL SDSSEQLIQ
