ID   CF251_TETTS             Reviewed;         996 AA.
AC   Q24DE2;
DT   12-APR-2017, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 2.
DT   25-MAY-2022, entry version 89.
DE   RecName: Full=Cilia- and flagella-associated protein 251 {ECO:0000305};
GN   Name=CFAP251 {ECO:0000305}; Synonyms=FAP251 {ECO:0000303|PubMed:25694453};
GN   ORFNames=TTHERM_01262850 {ECO:0000312|EMBL:EAS05799.2};
OS   Tetrahymena thermophila (strain SB210).
OC   Eukaryota; Sar; Alveolata; Ciliophora; Intramacronucleata;
OC   Oligohymenophorea; Hymenostomatida; Tetrahymenina; Tetrahymenidae;
OC   Tetrahymena.
OX   NCBI_TaxID=312017;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SB210;
RX   PubMed=16933976; DOI=10.1371/journal.pbio.0040286;
RA   Eisen J.A., Coyne R.S., Wu M., Wu D., Thiagarajan M., Wortman J.R.,
RA   Badger J.H., Ren Q., Amedeo P., Jones K.M., Tallon L.J., Delcher A.L.,
RA   Salzberg S.L., Silva J.C., Haas B.J., Majoros W.H., Farzad M.,
RA   Carlton J.M., Smith R.K. Jr., Garg J., Pearlman R.E., Karrer K.M., Sun L.,
RA   Manning G., Elde N.C., Turkewitz A.P., Asai D.J., Wilkes D.E., Wang Y.,
RA   Cai H., Collins K., Stewart B.A., Lee S.R., Wilamowska K., Weinberg Z.,
RA   Ruzzo W.L., Wloga D., Gaertig J., Frankel J., Tsao C.-C., Gorovsky M.A.,
RA   Keeling P.J., Waller R.F., Patron N.J., Cherry J.M., Stover N.A.,
RA   Krieger C.J., del Toro C., Ryder H.F., Williamson S.C., Barbeau R.A.,
RA   Hamilton E.P., Orias E.;
RT   "Macronuclear genome sequence of the ciliate Tetrahymena thermophila, a
RT   model eukaryote.";
RL   PLoS Biol. 4:1620-1642(2006).
RN   [2]
RP   FUNCTION, DISRUPTION PHENOTYPE, AND SUBCELLULAR LOCATION.
RX   PubMed=25694453; DOI=10.1091/mbc.e14-11-1545;
RA   Urbanska P., Song K., Joachimiak E., Krzemien-Ojak L., Koprowski P.,
RA   Hennessey T., Jerka-Dziadosz M., Fabczak H., Gaertig J., Nicastro D.,
RA   Wloga D.;
RT   "The CSC proteins FAP61 and FAP251 build the basal substructures of radial
RT   spoke 3 in cilia.";
RL   Mol. Biol. Cell 26:1463-1475(2015).
CC   -!- FUNCTION: As component of a spoke-associated complex, regulates ciliary
CC       mobility by mediating a stable and functional assembly of the radial
CC       spoke 3 (RS3). {ECO:0000269|PubMed:25694453}.
CC   -!- SUBCELLULAR LOCATION: Cell projection, cilium
CC       {ECO:0000269|PubMed:25694453}.
CC   -!- DISRUPTION PHENOTYPE: Knockout cells show deficiencies in cell
CC       multiplication and ciliary functions. Mutants move with greatly reduced
CC       velocities, have disturbed waveform of beating cilia and have stiffer
CC       cilia (PubMed:25694453). Mutant cilia lacjk an arch-like density of the
CC       radial spoke 3 base (PubMed:25694453). {ECO:0000269|PubMed:25694453}.
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DR   EMBL; GG662329; EAS05799.2; -; Genomic_DNA.
DR   RefSeq; XP_001026044.2; XM_001026044.2.
DR   AlphaFoldDB; Q24DE2; -.
DR   STRING; 5911.EAS05799; -.
DR   EnsemblProtists; EAS05799; EAS05799; TTHERM_01262850.
DR   GeneID; 7835487; -.
DR   KEGG; tet:TTHERM_01262850; -.
DR   eggNOG; ENOG502QQ05; Eukaryota.
DR   HOGENOM; CLU_007087_1_0_1; -.
DR   InParanoid; Q24DE2; -.
DR   OrthoDB; 169738at2759; -.
DR   Proteomes; UP000009168; Unassembled WGS sequence.
DR   GO; GO:0031514; C:motile cilium; IDA:UniProtKB.
DR   GO; GO:0003341; P:cilium movement; IMP:UniProtKB.
DR   GO; GO:0044782; P:cilium organization; IDA:UniProtKB.
DR   Gene3D; 2.130.10.10; -; 2.
DR   InterPro; IPR011992; EF-hand-dom_pair.
DR   InterPro; IPR011047; Quinoprotein_ADH-like_supfam.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR019775; WD40_repeat_CS.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   Pfam; PF00400; WD40; 2.
DR   SMART; SM00320; WD40; 9.
DR   SUPFAM; SSF47473; SSF47473; 1.
DR   SUPFAM; SSF50978; SSF50978; 1.
DR   SUPFAM; SSF50998; SSF50998; 1.
DR   PROSITE; PS00599; AA_TRANSFER_CLASS_2; 1.
DR   PROSITE; PS00678; WD_REPEATS_1; 1.
DR   PROSITE; PS50082; WD_REPEATS_2; 2.
PE   4: Predicted;
KW   Cell projection; Reference proteome; Repeat; WD repeat.
FT   CHAIN           1..996
FT                   /note="Cilia- and flagella-associated protein 251"
FT                   /id="PRO_0000439532"
FT   REPEAT          73..114
FT                   /note="WD 1"
FT                   /evidence="ECO:0000255"
FT   REPEAT          118..164
FT                   /note="WD 2"
FT                   /evidence="ECO:0000255"
FT   REPEAT          168..211
FT                   /note="WD 3"
FT                   /evidence="ECO:0000255"
FT   REPEAT          219..258
FT                   /note="WD 4"
FT                   /evidence="ECO:0000255"
FT   REPEAT          282..319
FT                   /note="WD 5"
FT                   /evidence="ECO:0000255"
FT   REPEAT          399..438
FT                   /note="WD 6"
FT                   /evidence="ECO:0000255"
FT   REPEAT          445..485
FT                   /note="WD 7"
FT                   /evidence="ECO:0000255"
FT   REPEAT          494..533
FT                   /note="WD 8"
FT                   /evidence="ECO:0000255"
FT   REPEAT          547..593
FT                   /note="WD 9"
FT                   /evidence="ECO:0000255"
FT   REPEAT          615..658
FT                   /note="WD 10"
FT                   /evidence="ECO:0000255"
FT   REPEAT          719..759
FT                   /note="WD 11"
FT                   /evidence="ECO:0000255"
FT   REGION          971..996
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   996 AA;  114443 MW;  CEC5545EFEED6365 CRC64;
     MYQQEEYEVN NEGQEDVSKI NALNLKWTLG FNFQLTDGVH NLTNEKRKEI FYPVAHTGVI
     YDYENNTQRL LQGHCNRITA TAYCKFSDII VTADCGPDSM LVVWDASTGI PKKTIFEPHP
     NGCQALDISQ DGQFIVTLSK EANTDTDVQS LSLWEWNKDD EPCLITNEFD RRIKDYQYFV
     RFNSDDNTEF ATTGKTRIVF WRREGEQKGF NYYSPGNIPS LKVLTQTVFI PDNTQVVTGT
     TDGHIIVWDI SLIIEKVDTP KERRAIKLVD LMNKSKKPDG KKGSNSINIL KIQDNYLVIG
     SSNGSIRFYD FQYRIIAWFE DAIIGSISNI SFANTPMIED NEDEDGFDDE RKDKGENEPK
     FICPDFIVVD LDATITMLNY KLFEELDDEK KKGTTLMKSI VSPIIAMSCR PNSNVIGICC
     ENGYLYEWNF QEKSSVLSIL RVFDTDKENI PNCIDYSPDG NWLSVATKSG KIHIFDCKEK
     QWQNSVLEVS ENEQGKPKVN MQVFSSDSKN LATMDADYAV SLFTIDHRQF DVNIPEKEWQ
     FVGKHRIHHS EIKSIAFGES KNENDKKYYR LFSIGADMKM VEYEVLEIDY SKQKEKGQQI
     NYIDRLKLKS IYPIEQETIP TACIWYPINM YKEDVLLTVN EEFKIKLWQF MKDNFKFCKK
     TCLGPIYGGA INKLLILNQN DAQNDYQDKY LAYSTKEKVV GIIKLPLEGN PNQTMGLIAH
     PDKITSISCS NDGKHFFSSG SDDYCVNVWS VNVQALEQIF ATNPNEDPYP KLLEGGEDGQ
     THQDLKNFFY YSQIRSKDEH TTKARKLDGK VPLIAIPDMM RSMGYYPTNK EIENMQNEIR
     FSKYLDPGEQ VEELDLNMFL KLFVNHRPVQ GIGKSKIAES LNTLTKSLSD SAKEIAEQAK
     GQSVQPKTYN IDGIGEISLQ DLKKILTTEG ERMTNEEFDE CLKILCGENE DQIPQQINVN
     NLCEDILGFE DLEGEERDDN IEDQYEDEEN EEYDQD