ID   CF300_CHLRE             Reviewed;         257 AA.
AC   A8IYS6;
DT   18-JUL-2018, integrated into UniProtKB/Swiss-Prot.
DT   04-DEC-2007, sequence version 1.
DT   03-AUG-2022, entry version 52.
DE   RecName: Full=Cilia- and flagella-associated protein 300 {ECO:0000305};
DE   AltName: Full=Flagellar and basal body protein;
GN   Name=CFAP300 {ECO:0000250|UniProtKB:Q9BRQ4}; Synonyms=FBB5;
GN   ORFNames=CHLRE_12g556300v5 {ECO:0000305}, CHLREDRAFT_190937;
OS   Chlamydomonas reinhardtii (Chlamydomonas smithii).
OC   Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC   CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX   NCBI_TaxID=3055;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CC-503;
RX   PubMed=17932292; DOI=10.1126/science.1143609;
RA   Merchant S.S., Prochnik S.E., Vallon O., Harris E.H., Karpowicz S.J.,
RA   Witman G.B., Terry A., Salamov A., Fritz-Laylin L.K., Marechal-Drouard L.,
RA   Marshall W.F., Qu L.H., Nelson D.R., Sanderfoot A.A., Spalding M.H.,
RA   Kapitonov V.V., Ren Q., Ferris P., Lindquist E., Shapiro H., Lucas S.M.,
RA   Grimwood J., Schmutz J., Cardol P., Cerutti H., Chanfreau G., Chen C.L.,
RA   Cognat V., Croft M.T., Dent R., Dutcher S., Fernandez E., Fukuzawa H.,
RA   Gonzalez-Ballester D., Gonzalez-Halphen D., Hallmann A., Hanikenne M.,
RA   Hippler M., Inwood W., Jabbari K., Kalanon M., Kuras R., Lefebvre P.A.,
RA   Lemaire S.D., Lobanov A.V., Lohr M., Manuell A., Meier I., Mets L.,
RA   Mittag M., Mittelmeier T., Moroney J.V., Moseley J., Napoli C.,
RA   Nedelcu A.M., Niyogi K., Novoselov S.V., Paulsen I.T., Pazour G.J.,
RA   Purton S., Ral J.P., Riano-Pachon D.M., Riekhof W., Rymarquis L.,
RA   Schroda M., Stern D., Umen J., Willows R., Wilson N., Zimmer S.L.,
RA   Allmer J., Balk J., Bisova K., Chen C.J., Elias M., Gendler K., Hauser C.,
RA   Lamb M.R., Ledford H., Long J.C., Minagawa J., Page M.D., Pan J.,
RA   Pootakham W., Roje S., Rose A., Stahlberg E., Terauchi A.M., Yang P.,
RA   Ball S., Bowler C., Dieckmann C.L., Gladyshev V.N., Green P., Jorgensen R.,
RA   Mayfield S., Mueller-Roeber B., Rajamani S., Sayre R.T., Brokstein P.,
RA   Dubchak I., Goodstein D., Hornick L., Huang Y.W., Jhaveri J., Luo Y.,
RA   Martinez D., Ngau W.C., Otillar B., Poliakov A., Porter A., Szajkowski L.,
RA   Werner G., Zhou K., Grigoriev I.V., Rokhsar D.S., Grossman A.R.;
RT   "The Chlamydomonas genome reveals the evolution of key animal and plant
RT   functions.";
RL   Science 318:245-250(2007).
RN   [2]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=29727692; DOI=10.1016/j.ajhg.2018.03.024;
RA   Fassad M.R., Shoemark A., le Borgne P., Koll F., Patel M., Dixon M.,
RA   Hayward J., Richardson C., Frost E., Jenkins L., Cullup T., Chung E.M.K.,
RA   Lemullois M., Aubusson-Fleury A., Hogg C., Mitchell D.R., Tassin A.M.,
RA   Mitchison H.M.;
RT   "C11orf70 mutations disrupting the intraflagellar transport-dependent
RT   assembly of multiple axonemal dyneins cause primary ciliary dyskinesia.";
RL   Am. J. Hum. Genet. 102:956-972(2018).
CC   -!- FUNCTION: Cilium- and flagellum-specific protein that plays a role in
CC       axonemal structure organization and motility. Plays a role in outer and
CC       inner dynein arm assembly. {ECO:0000269|PubMed:29727692}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:29727692}.
CC       Cytoplasm, cytoskeleton, flagellum axoneme
CC       {ECO:0000269|PubMed:29727692}. Note=Localizes predominantly in the
CC       cytoplasm and weakly in flagella. Transported to the flagellar matrix
CC       in an intraflagellar transport (IFT)-dependent manner.
CC       {ECO:0000269|PubMed:29727692}.
CC   -!- SIMILARITY: Belongs to the CFAP300 family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; DS496127; EDP02986.1; -; Genomic_DNA.
DR   EMBL; CM008973; PNW75890.1; -; Genomic_DNA.
DR   RefSeq; XP_001694050.1; XM_001693998.1.
DR   AlphaFoldDB; A8IYS6; -.
DR   STRING; 3055.EDP02986; -.
DR   PaxDb; A8IYS6; -.
DR   PRIDE; A8IYS6; -.
DR   EnsemblPlants; PNW75890; PNW75890; CHLRE_12g556300v5.
DR   GeneID; 5719752; -.
DR   Gramene; PNW75890; PNW75890; CHLRE_12g556300v5.
DR   KEGG; cre:CHLRE_12g556300v5; -.
DR   eggNOG; ENOG502QUFH; Eukaryota.
DR   HOGENOM; CLU_068703_0_0_1; -.
DR   InParanoid; A8IYS6; -.
DR   OMA; VVPCTQI; -.
DR   OrthoDB; 1089634at2759; -.
DR   Proteomes; UP000006906; Chromosome 12.
DR   GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR   GO; GO:0005856; C:cytoskeleton; IEA:UniProtKB-KW.
DR   GO; GO:0031514; C:motile cilium; IDA:UniProtKB.
DR   InterPro; IPR029416; CFAP300.
DR   PANTHER; PTHR31078; PTHR31078; 1.
DR   Pfam; PF14926; DUF4498; 1.
PE   3: Inferred from homology;
KW   Cell projection; Cilium; Cytoplasm; Cytoskeleton; Flagellum;
KW   Reference proteome.
FT   CHAIN           1..257
FT                   /note="Cilia- and flagella-associated protein 300"
FT                   /id="PRO_0000444630"
SQ   SEQUENCE   257 AA;  29163 MW;  C2E9FFA92A8E0A67 CRC64;
     MTAFVPVSLP STSALNDAYV KSQLTKWDLL RNLRCVAVRY TKYYHKLQGQ ELLADLFRDE
     KVQEAFQVLR KGGAWGQLGG PVTKVDATLL ASSLTRMDLF DKLTETSPPI VRSNGDIGKC
     MEDNREGFQV SDQLRELILV EESEHAALFS EAERDELLWR LFEHVVLGGA CCQFEDKVEP
     YVETSKRLYK ELVCAQKDPA TGKVQTVSAV YKINSIQGDS GPLELYPSRS RQNFCYAAVD
     PVRRIVKILY HAYVPYW