ID   CF300_PARTE             Reviewed;         252 AA.
AC   A0CY51;
DT   18-JUL-2018, integrated into UniProtKB/Swiss-Prot.
DT   28-NOV-2006, sequence version 1.
DT   25-MAY-2022, entry version 41.
DE   RecName: Full=Cilia- and flagella-associated protein 300 {ECO:0000305};
GN   Name=CFAP300 {ECO:0000250|UniProtKB:Q9BRQ4}; ORFNames=GSPATT00011350001;
OS   Paramecium tetraurelia.
OC   Eukaryota; Sar; Alveolata; Ciliophora; Intramacronucleata;
OC   Oligohymenophorea; Peniculida; Parameciidae; Paramecium.
OX   NCBI_TaxID=5888;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Stock d4-2;
RX   PubMed=17086204; DOI=10.1038/nature05230;
RA   Aury J.-M., Jaillon O., Duret L., Noel B., Jubin C., Porcel B.M.,
RA   Segurens B., Daubin V., Anthouard V., Aiach N., Arnaiz O., Billaut A.,
RA   Beisson J., Blanc I., Bouhouche K., Camara F., Duharcourt S., Guigo R.,
RA   Gogendeau D., Katinka M., Keller A.-M., Kissmehl R., Klotz C., Koll F.,
RA   Le Mouel A., Lepere G., Malinsky S., Nowacki M., Nowak J.K., Plattner H.,
RA   Poulain J., Ruiz F., Serrano V., Zagulski M., Dessen P., Betermier M.,
RA   Weissenbach J., Scarpelli C., Schaechter V., Sperling L., Meyer E.,
RA   Cohen J., Wincker P.;
RT   "Global trends of whole-genome duplications revealed by the ciliate
RT   Paramecium tetraurelia.";
RL   Nature 444:171-178(2006).
RN   [2]
RP   FUNCTION, SUBCELLULAR LOCATION, INDUCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=29727692; DOI=10.1016/j.ajhg.2018.03.024;
RA   Fassad M.R., Shoemark A., le Borgne P., Koll F., Patel M., Dixon M.,
RA   Hayward J., Richardson C., Frost E., Jenkins L., Cullup T., Chung E.M.K.,
RA   Lemullois M., Aubusson-Fleury A., Hogg C., Mitchell D.R., Tassin A.M.,
RA   Mitchison H.M.;
RT   "C11orf70 mutations disrupting the intraflagellar transport-dependent
RT   assembly of multiple axonemal dyneins cause primary ciliary dyskinesia.";
RL   Am. J. Hum. Genet. 102:956-972(2018).
CC   -!- FUNCTION: Cilium- and flagellum-specific protein that plays a role in
CC       axonemal structure organization and motility. Plays a role in outer and
CC       inner axonemal dynein arm assembly. {ECO:0000269|PubMed:29727692}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:29727692}.
CC       Cytoplasm, cytoskeleton, cilium axoneme {ECO:0000269|PubMed:29727692}.
CC       Note=Localizes predominantly in the cytoplasm and weakly in cilia.
CC       Transported within cilia in an intraflagellar transport (IFT)-dependent
CC       manner. Accumulates at the ciliary tips during ciliogenesis.
CC       {ECO:0000269|PubMed:29727692}.
CC   -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown of the protein causes
CC       reduced swimming motility and velocity. Display a reduction in the
CC       cilia beating frequency and an altered cilia waveform. Show absence of
CC       the outer and inner dynein arms. {ECO:0000269|PubMed:29727692}.
CC   -!- SIMILARITY: Belongs to the CFAP300 family. {ECO:0000305}.
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DR   EMBL; CT868208; CAK75718.1; -; Genomic_DNA.
DR   RefSeq; XP_001443115.1; XM_001443078.1.
DR   AlphaFoldDB; A0CY51; -.
DR   STRING; 5888.CAK75718; -.
DR   EnsemblProtists; CAK75718; CAK75718; GSPATT00011350001.
DR   GeneID; 5028900; -.
DR   KEGG; ptm:GSPATT00011350001; -.
DR   eggNOG; ENOG502QUFH; Eukaryota.
DR   HOGENOM; CLU_068703_0_0_1; -.
DR   InParanoid; A0CY51; -.
DR   OMA; VVPCTQI; -.
DR   Proteomes; UP000000600; Partially assembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR   GO; GO:0005856; C:cytoskeleton; IEA:UniProtKB-KW.
DR   GO; GO:0031514; C:motile cilium; IDA:UniProtKB.
DR   GO; GO:0036159; P:inner dynein arm assembly; IMP:UniProtKB.
DR   GO; GO:0036158; P:outer dynein arm assembly; IMP:UniProtKB.
DR   GO; GO:0120030; P:positive regulation of cilium beat frequency involved in ciliary motility; IMP:UniProtKB.
DR   InterPro; IPR029416; CFAP300.
DR   PANTHER; PTHR31078; PTHR31078; 1.
DR   Pfam; PF14926; DUF4498; 1.
PE   2: Evidence at transcript level;
KW   Cell projection; Cytoplasm; Cytoskeleton; Reference proteome.
FT   CHAIN           1..252
FT                   /note="Cilia- and flagella-associated protein 300"
FT                   /id="PRO_0000444629"
SQ   SEQUENCE   252 AA;  30046 MW;  31CAB353602ECCA1 CRC64;
     MQIESDNQVT NQSYSFFRQE NTILDDKKFM EILQKWGLQH SIKVSTFLFD IKFDHLNPNQ
     FLLDLFNSKD VRGSLHYVSF KQNVLLSQIK FQPLTCKSIK LDLFDKLTED KIVVKGHIKQ
     CFEEQFENIQ IADELRKALV LEDSEQYCVF NEADRQELLF KLFQILVLGG QLCQYEDEIQ
     AYLDWTKYLY KNTVNARKYA DKDEIYIDSY AYDIRKLENS YSSDHPQNVM YVVVNPSLRI
     VNIIENQWLK VW