ID   CF4_COTFL               Reviewed;          54 AA.
AC   P0DQT4;
DT   25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT   25-MAY-2022, sequence version 1.
DT   03-AUG-2022, entry version 2.
DE   RecName: Full=H-bracotoxin-Cf4 {ECO:0000303|PubMed:34547307};
DE            Short=H-BCTX-Cf4 {ECO:0000303|PubMed:34547307};
DE   Flags: Precursor;
OS   Cotesia flavipes (Parasitic wasp) (Apanteles flavipes).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Hymenoptera; Apocrita; Parasitoida;
OC   Ichneumonoidea; Braconidae; Microgastrinae; Cotesia.
OX   NCBI_TaxID=89805;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SYNTHESIS OF 22-54, AND
RP   BIOTECHNOLOGY.
RX   PubMed=34547307; DOI=10.1016/j.toxicon.2021.09.002;
RA   Pinto C.P.G., Walker A.A., Robinson S.D., Chin Y.K., King G.F., Rossi G.D.;
RT   "Venom composition of the endoparasitoid wasp Cotesia flavipes
RT   (Hymenoptera: Braconidae) and functional characterization of a major venom
RT   peptide.";
RL   Toxicon 202:1-12(2021).
CC   -!- FUNCTION: This endoparasitoid wasp peptide has a role in disruption of
CC       the cellular host immune response, since it reduces the capacity of
CC       D.saccharalis hemocytes to encapsulate foreign bodies. On the other
CC       hand, it shows no effect on the humoral immune response, since it has
CC       no effect on phenoloxidase activity. {ECO:0000269|PubMed:34547307}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:34547307}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom duct.
CC       {ECO:0000305|PubMed:34547307}.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin. {ECO:0000305}.
CC   -!- BIOTECHNOLOGY: Potential candidate for insect pest control through
CC       ingestion. Feeding leaves coated with this peptide to neonate
CC       D.saccharalis result in increased mortality and significantly reduced
CC       feeding compared to caterpillars fed untreated leaves.
CC       {ECO:0000269|PubMed:34547307}.
CC   -!- MISCELLANEOUS: Highly abundant in venom. {ECO:0000269|PubMed:34547307}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; MZ442218; UEP64248.1; -; mRNA.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
PE   1: Evidence at protein level;
KW   Disulfide bond; Knottin; Secreted; Signal.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   CHAIN           22..54
FT                   /note="H-bracotoxin-Cf4"
FT                   /evidence="ECO:0000305|PubMed:34547307"
FT                   /id="PRO_0000455164"
FT   DISULFID        24..39
FT                   /evidence="ECO:0000305|PubMed:34547307"
FT   DISULFID        31..43
FT                   /evidence="ECO:0000305|PubMed:34547307"
FT   DISULFID        38..53
FT                   /evidence="ECO:0000305|PubMed:34547307"
SQ   SEQUENCE   54 AA;  5740 MW;  DCD722E26EF8FC68 CRC64;
     MSKLFIFFLL VALLAFVSSE AAECVAKGQN CIVGKSTCCI GQCIIKDHVL GSCE