CF50_DICDI
ID CF50_DICDI Reviewed; 303 AA.
AC Q556R7; Q86JX6; Q95VT3;
DT 29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 02-MAY-2006, sequence version 1.
DT 25-MAY-2022, entry version 96.
DE RecName: Full=Counting factor 50;
DE EC=3.2.1.17;
DE AltName: Full=1,4-beta-N-acetylmuramidase 1;
DE AltName: Full=GH family 25 lysozyme 1;
DE Flags: Precursor;
GN Name=cf50-1; ORFNames=DDB_G0273175;
GN and
GN Name=cf50-2; ORFNames=DDB_G0273875;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 25-42,
RP IDENTIFICATION IN THE CF COMPLEX, SUBUNIT, AND DISRUPTION PHENOTYPE.
RX PubMed=12117815; DOI=10.1242/dev.129.15.3657;
RA Brock D.A., Hatton R.D., Giurgiutiu D.-V., Scott B., Ammann R., Gomer R.H.;
RT "The different components of a multisubunit cell number-counting factor
RT have both unique and overlapping functions.";
RL Development 129:3657-3668(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=12097910; DOI=10.1038/nature00847;
RA Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA Noegel A.A.;
RT "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL Nature 418:79-85(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [4]
RP IDENTIFICATION IN THE CF COMPLEX.
RX PubMed=10444594; DOI=10.1101/gad.13.15.1960;
RA Brock D.A., Gomer R.H.;
RT "A cell-counting factor regulating structure size in Dictyostelium.";
RL Genes Dev. 13:1960-1969(1999).
RN [5]
RP IDENTIFICATION IN THE CF COMPLEX.
RX PubMed=12912898; DOI=10.1128/ec.2.4.788-797.2003;
RA Brock D.A., Hatton R.D., Giurgiutiu D.-V., Scott B., Jang W., Ammann R.,
RA Gomer R.H.;
RT "CF45-1, a secreted protein which participates in Dictyostelium group size
RT regulation.";
RL Eukaryot. Cell 2:788-797(2003).
RN [6]
RP IDENTIFICATION IN THE CF COMPLEX.
RX PubMed=16963635; DOI=10.1128/ec.00169-06;
RA Brock D.A., van Egmond W.N., Shamoo Y., Hatton R.D., Gomer R.H.;
RT "A 60-kilodalton protein component of the counting factor complex regulates
RT group size in Dictyostelium discoideum.";
RL Eukaryot. Cell 5:1532-1538(2006).
CC -!- FUNCTION: Cell-counting factor that limits the maximum size of the
CC multicellular structure during aggregation. Has a very low lysozyme
CC activity.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic
CC acid and N-acetyl-D-glucosamine residues in a peptidoglycan and
CC between N-acetyl-D-glucosamine residues in chitodextrins.;
CC EC=3.2.1.17;
CC -!- SUBUNIT: Monomer. Component of the counting factor (CF) complex, which
CC includes cf60, cf50, cf45-1 and ctnA. {ECO:0000269|PubMed:10444594,
CC ECO:0000269|PubMed:12117815, ECO:0000269|PubMed:12912898,
CC ECO:0000269|PubMed:16963635}.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- DISRUPTION PHENOTYPE: In the absence of CF50, secreted countin is
CC degraded suggesting that it may protect countin from degradation.
CC {ECO:0000269|PubMed:12117815}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 25 family.
CC {ECO:0000255|PROSITE-ProRule:PRU01252, ECO:0000305}.
CC -!- CAUTION: The gene for this protein is duplicated in strains AX3 and
CC AX4. These strains contain a duplication of a segment of 750 kb of
CC chromosome 2 compared to the corresponding sequence in strain AX2.
CC {ECO:0000305}.
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DR EMBL; AF405695; AAL01036.1; -; Genomic_DNA.
DR EMBL; AAFI02000011; EAL70628.1; -; Genomic_DNA.
DR EMBL; AAFI02000009; EAL70805.1; -; Genomic_DNA.
DR RefSeq; XP_644554.1; XM_639462.1.
DR RefSeq; XP_644697.1; XM_639605.1.
DR AlphaFoldDB; Q556R7; -.
DR SMR; Q556R7; -.
DR PaxDb; Q556R7; -.
DR EnsemblProtists; EAL70628; EAL70628; DDB_G0273875.
DR EnsemblProtists; EAL70805; EAL70805; DDB_G0273175.
DR GeneID; 8618796; -.
DR GeneID; 8619180; -.
DR KEGG; ddi:DDB_G0273175; -.
DR KEGG; ddi:DDB_G0273875; -.
DR dictyBase; DDB_G0273175; cf50-1.
DR dictyBase; DDB_G0273875; cf50-2.
DR HOGENOM; CLU_073372_3_0_1; -.
DR InParanoid; Q556R7; -.
DR PhylomeDB; Q556R7; -.
DR PRO; PR:Q556R7; -.
DR Proteomes; UP000002195; Chromosome 2.
DR GO; GO:0005576; C:extracellular region; IDA:dictyBase.
DR GO; GO:0003796; F:lysozyme activity; IEA:UniProtKB-EC.
DR GO; GO:0030154; P:cell differentiation; IMP:dictyBase.
DR GO; GO:0048870; P:cell motility; IMP:dictyBase.
DR GO; GO:0016998; P:cell wall macromolecule catabolic process; IEA:InterPro.
DR GO; GO:0098609; P:cell-cell adhesion; IMP:dictyBase.
DR GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
DR GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR GO; GO:0042593; P:glucose homeostasis; IMP:dictyBase.
DR GO; GO:0031158; P:negative regulation of aggregate size involved in sorocarp development; IMP:dictyBase.
DR GO; GO:0045861; P:negative regulation of proteolysis; IMP:dictyBase.
DR GO; GO:0009253; P:peptidoglycan catabolic process; IEA:InterPro.
DR GO; GO:0007165; P:signal transduction; IMP:dictyBase.
DR InterPro; IPR002053; Glyco_hydro_25.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR Pfam; PF01183; Glyco_hydro_25; 1.
DR SUPFAM; SSF51445; SSF51445; 1.
DR PROSITE; PS51904; GLYCOSYL_HYDROL_F25_2; 1.
PE 1: Evidence at protein level;
KW Antimicrobial; Bacteriolytic enzyme; Direct protein sequencing;
KW Glycoprotein; Glycosidase; Hydrolase; Reference proteome; Repeat; Secreted;
KW Signal.
FT SIGNAL 1..24
FT /evidence="ECO:0000269|PubMed:12117815"
FT CHAIN 25..303
FT /note="Counting factor 50"
FT /id="PRO_0000330647"
FT DOMAIN 28..240
FT /note="Ch-type lysozyme"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01252"
FT REGION 226..303
FT /note="S-G-S motif repeats"
FT REGION 236..303
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 125
FT /evidence="ECO:0000250"
FT CARBOHYD 67
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 170
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 303 AA; 30854 MW; 2B2A3CC1CEE3CE8A CRC64;
MNKMNNIFLI ISSIILSIVI FVSGECAIDF SSEISVGISD SQWSCLASNN QRVIIQVWSG
GGQYNSNISS VVSAAEQAGF DNIDLYAFLC SECDGNYPAS SAIQSLVSSL KSDGINFNML
WIDVEQCDGC WGAESDNADY VQEAVETAQG LGVLVGVYSS EGEWPQTVGN LSTLSQYPLW
YAHYDDNPSF SDTAFYEFGG WTSPAMKQYI GNTNQCGVSV DLDFYGSGSG CSTSSGSASG
SASGSASGSA SGSNSGSSNS GSSNSGSSNS GSNSGSSNSG SGNSGSSNSG SASGSGTGSG
SSI
