CF60_DICDI
ID CF60_DICDI Reviewed; 416 AA.
AC Q54PR9; Q3YBY3;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 25-MAY-2022, entry version 90.
DE RecName: Full=Counting factor 60;
DE Flags: Precursor;
GN Name=cf60; ORFNames=DDB_G0284363;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 300-314, FUNCTION,
RP SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, IDENTIFICATION IN THE CF
RP COMPLEX, AND DISRUPTION PHENOTYPE.
RX PubMed=16963635; DOI=10.1128/ec.00169-06;
RA Brock D.A., van Egmond W.N., Shamoo Y., Hatton R.D., Gomer R.H.;
RT "A 60-kilodalton protein component of the counting factor complex regulates
RT group size in Dictyostelium discoideum.";
RL Eukaryot. Cell 5:1532-1538(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: Cell-counting factor that limits the maximum size of the
CC multicellular structure. Does not possess acid phosphatase activity.
CC Cells with decreased levels of this protein form large groups while
CC cells overexpressing this protein form small groups.
CC {ECO:0000269|PubMed:16963635}.
CC -!- SUBUNIT: Component of the counting factor (CF) complex, which includes
CC cf60, cf50, cf45-1 and ctnA. {ECO:0000269|PubMed:16963635}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:16963635}.
CC -!- DEVELOPMENTAL STAGE: Expressed in the vegetative cells and decreases
CC during subsequent developmental stages (6-24 hours).
CC {ECO:0000269|PubMed:16963635}.
CC -!- DISRUPTION PHENOTYPE: Cells die quickly. {ECO:0000269|PubMed:16963635}.
CC -!- SIMILARITY: Belongs to the histidine acid phosphatase family.
CC {ECO:0000305}.
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DR EMBL; DQ143912; AAZ73157.1; -; mRNA.
DR EMBL; AAFI02000064; EAL65230.1; -; Genomic_DNA.
DR RefSeq; XP_638585.1; XM_633493.1.
DR AlphaFoldDB; Q54PR9; -.
DR SMR; Q54PR9; -.
DR STRING; 44689.DDB0229833; -.
DR PaxDb; Q54PR9; -.
DR EnsemblProtists; EAL65230; EAL65230; DDB_G0284363.
DR GeneID; 8624557; -.
DR KEGG; ddi:DDB_G0284363; -.
DR dictyBase; DDB_G0284363; cf60.
DR eggNOG; KOG3720; Eukaryota.
DR HOGENOM; CLU_030431_5_0_1; -.
DR InParanoid; Q54PR9; -.
DR OMA; SWPPFTS; -.
DR PhylomeDB; Q54PR9; -.
DR Reactome; R-DDI-6798695; Neutrophil degranulation.
DR PRO; PR:Q54PR9; -.
DR Proteomes; UP000002195; Chromosome 4.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0016791; F:phosphatase activity; IBA:GO_Central.
DR GO; GO:0016311; P:dephosphorylation; IBA:GO_Central.
DR GO; GO:0031158; P:negative regulation of aggregate size involved in sorocarp development; IDA:dictyBase.
DR CDD; cd07061; HP_HAP_like; 1.
DR Gene3D; 3.40.50.1240; -; 1.
DR InterPro; IPR033379; Acid_Pase_AS.
DR InterPro; IPR000560; His_Pase_clade-2.
DR InterPro; IPR029033; His_PPase_superfam.
DR Pfam; PF00328; His_Phos_2; 1.
DR SUPFAM; SSF53254; SSF53254; 1.
DR PROSITE; PS00616; HIS_ACID_PHOSPHAT_1; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Glycoprotein; Reference proteome; Secreted;
KW Signal.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..416
FT /note="Counting factor 60"
FT /id="PRO_0000328378"
FT CARBOHYD 110
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 218
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 231
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 318
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 411
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 416 AA; 46755 MW; 18472B3C2927427F CRC64;
MIKKSALITL FLVSLILGVS LSQKPFYCQA PEPTPSLNTD GLTLKMVQIL TRHGDRTPLY
STLKPTMNTW DCNLGWLMVS SLNNVPGAAT DVDRLFRKVY MPNREYFPGN CSDGQLTSLG
FQQHLQLGQS LRQLYVDKYE LLPSELSVDA ASTIWVRSTD VPRTIQSVQG HLTALFPPTT
VTSGSGIPII NINTMDNYYE NMTPNPTLCP ELAVLIANTT TTPEWGEFIT NTTQLKEDVM
ETLGISVFPG WSSLMDLFFA TQCHDFPLPE GVTQDMVTQV YEAAYWQYQY QLSFPMIARL
GMSTFLEEVV DNIRAFVNGT SSVKYIVFSG HDDSVGPFTN LFGLMKEWPP YASHVELELW
SDEKDNYFLQ FKFNGQSYTL NGCEDVMCPI DSFFETAYSI LVPNYADACS NSTMTF
