ID   CFA20_BOVIN             Reviewed;         193 AA.
AC   Q6B857;
DT   24-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2004, sequence version 1.
DT   03-AUG-2022, entry version 87.
DE   RecName: Full=Cilia- and flagella-associated protein 20;
GN   Name=CFAP20; Synonyms=GTL3 {ECO:0000303|PubMed:15803458};
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND DEVELOPMENTAL STAGE.
RX   PubMed=15803458; DOI=10.1002/mrd.20282;
RA   Hwang K.-C., Park S.-Y., Park S.-P., Lim J.H., Cui X.-S., Kim N.-H.;
RT   "Specific maternal transcripts in bovine oocytes and cleavaged embryos:
RT   identification with novel DDRT-PCR methods.";
RL   Mol. Reprod. Dev. 71:275-283(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Heart ventricle;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases.
RN   [3] {ECO:0007744|PDB:7RRO}
RP   STRUCTURE BY ELECTRON MICROSCOPY (3.40 ANGSTROMS), FUNCTION, SUBCELLULAR
RP   LOCATION, AND TISSUE SPECIFICITY.
RX   PubMed=34715025; DOI=10.1016/j.cell.2021.10.007;
RA   Gui M., Farley H., Anujan P., Anderson J.R., Maxwell D.W., Whitchurch J.B.,
RA   Botsch J.J., Qiu T., Meleppattu S., Singh S.K., Zhang Q., Thompson J.,
RA   Lucas J.S., Bingle C.D., Norris D.P., Roy S., Brown A.;
RT   "De novo identification of mammalian ciliary motility proteins using cryo-
RT   EM.";
RL   Cell 184:5791-5806.e19(2021).
CC   -!- FUNCTION: Cilium- and flagellum-specific protein that plays a role in
CC       axonemal structure organization and motility (PubMed:34715025).
CC       Involved in the regulation of the size and morphology of cilia.
CC       Required for axonemal microtubules polyglutamylation (By similarity).
CC       {ECO:0000250|UniProtKB:Q9Y6A4, ECO:0000269|PubMed:34715025}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9Y6A4}.
CC       Cytoplasm, cytoskeleton, microtubule organizing center, centrosome,
CC       centriole {ECO:0000250|UniProtKB:Q9Y6A4}. Cytoplasm, cytoskeleton,
CC       cilium basal body {ECO:0000250|UniProtKB:Q9Y6A4}. Cytoplasm,
CC       cytoskeleton, cilium axoneme {ECO:0000269|PubMed:34715025}.
CC       Note=Microtubule inner protein (MIP) part of the dynein-decorated
CC       doublet microtubules (DMTs) in cilia axoneme.
CC       {ECO:0000269|PubMed:34715025}.
CC   -!- TISSUE SPECIFICITY: Expressed in trachea multiciliated cells.
CC       {ECO:0000269|PubMed:34715025}.
CC   -!- DEVELOPMENTAL STAGE: Expressed at high levels in germinal vesicle stage
CC       oocytes at the mRNA level. Expression peaks in 2-cell embryos and
CC       decreases thereafter. Hardly detectable in morula stage embryos. This
CC       pattern of expression suggests a maternally derived transcript.
CC       {ECO:0000269|PubMed:15803458}.
CC   -!- SIMILARITY: Belongs to the CFAP20 family. {ECO:0000305}.
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DR   EMBL; AY675080; AAT84373.1; -; mRNA.
DR   EMBL; BC112831; AAI12832.1; -; mRNA.
DR   RefSeq; NP_001003905.1; NM_001003905.1.
DR   PDB; 7RRO; EM; 3.40 A; XA/XB/XC/XD/XE/XF/XG=1-193.
DR   PDBsum; 7RRO; -.
DR   AlphaFoldDB; Q6B857; -.
DR   SMR; Q6B857; -.
DR   STRING; 9913.ENSBTAP00000018422; -.
DR   PaxDb; Q6B857; -.
DR   PRIDE; Q6B857; -.
DR   Ensembl; ENSBTAT00000018422; ENSBTAP00000018422; ENSBTAG00000013874.
DR   GeneID; 445424; -.
DR   KEGG; bta:445424; -.
DR   CTD; 29105; -.
DR   VEuPathDB; HostDB:ENSBTAG00000013874; -.
DR   VGNC; VGNC:27240; CFAP20.
DR   eggNOG; KOG3213; Eukaryota.
DR   GeneTree; ENSGT00390000004554; -.
DR   HOGENOM; CLU_060610_1_1_1; -.
DR   InParanoid; Q6B857; -.
DR   OMA; TTYISCP; -.
DR   OrthoDB; 1213295at2759; -.
DR   TreeFam; TF313405; -.
DR   Proteomes; UP000009136; Chromosome 18.
DR   Bgee; ENSBTAG00000013874; Expressed in oocyte and 107 other tissues.
DR   GO; GO:0005879; C:axonemal microtubule; IDA:UniProtKB.
DR   GO; GO:0005814; C:centriole; ISS:UniProtKB.
DR   GO; GO:0036064; C:ciliary basal body; ISS:UniProtKB.
DR   GO; GO:0005929; C:cilium; ISS:UniProtKB.
DR   GO; GO:0031514; C:motile cilium; IBA:GO_Central.
DR   GO; GO:0005654; C:nucleoplasm; IEA:Ensembl.
DR   GO; GO:0060271; P:cilium assembly; ISS:UniProtKB.
DR   GO; GO:2000147; P:positive regulation of cell motility; ISS:UniProtKB.
DR   GO; GO:2000253; P:positive regulation of feeding behavior; ISS:UniProtKB.
DR   GO; GO:0018095; P:protein polyglutamylation; ISS:UniProtKB.
DR   GO; GO:0060296; P:regulation of cilium beat frequency involved in ciliary motility; ISS:UniProtKB.
DR   InterPro; IPR040441; CFA20/CFAP20DC.
DR   InterPro; IPR007714; CFA20_dom.
DR   PANTHER; PTHR12458; PTHR12458; 1.
DR   Pfam; PF05018; DUF667; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cell projection; Cilium; Cytoplasm; Cytoskeleton;
KW   Microtubule; Nucleus; Reference proteome.
FT   CHAIN           1..193
FT                   /note="Cilia- and flagella-associated protein 20"
FT                   /id="PRO_0000296397"
SQ   SEQUENCE   193 AA;  22748 MW;  9DF3DB123A15BF77 CRC64;
     MFKNTFQSGF LSILYSIGSK PLQIWDKKVR NGHIKRITDN DIQSLVLEIE GTNVSTTYIT
     CPADPKKTLG IKLPFLVMII KNLKKYFTFE VQVLDDKNVR RRFRASNYQS TTRVKPFICT
     MPMRLDDGWN QIQFNLSDFT RRAYGTNYIE TLRVQIHANC RIRRVYFSDR LYSEDELPAE
     FKLYLPVQNK AKQ