ID   CFA20_CHLRE             Reviewed;         190 AA.
AC   A8IU92;
DT   01-OCT-2014, integrated into UniProtKB/Swiss-Prot.
DT   04-DEC-2007, sequence version 1.
DT   25-MAY-2022, entry version 55.
DE   RecName: Full=Cilia- and flagella-associated protein 20;
DE   AltName: Full=Basal body up-regulated protein 22;
DE            Short=Bug22p;
DE   AltName: Full=Flagellar-associated protein 20;
GN   Name=CFAP20; Synonyms=BUG22, FAP20; ORFNames=CHLREDRAFT_189631;
OS   Chlamydomonas reinhardtii (Chlamydomonas smithii).
OC   Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC   CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX   NCBI_TaxID=3055;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CC-503;
RX   PubMed=17932292; DOI=10.1126/science.1143609;
RA   Merchant S.S., Prochnik S.E., Vallon O., Harris E.H., Karpowicz S.J.,
RA   Witman G.B., Terry A., Salamov A., Fritz-Laylin L.K., Marechal-Drouard L.,
RA   Marshall W.F., Qu L.H., Nelson D.R., Sanderfoot A.A., Spalding M.H.,
RA   Kapitonov V.V., Ren Q., Ferris P., Lindquist E., Shapiro H., Lucas S.M.,
RA   Grimwood J., Schmutz J., Cardol P., Cerutti H., Chanfreau G., Chen C.L.,
RA   Cognat V., Croft M.T., Dent R., Dutcher S., Fernandez E., Fukuzawa H.,
RA   Gonzalez-Ballester D., Gonzalez-Halphen D., Hallmann A., Hanikenne M.,
RA   Hippler M., Inwood W., Jabbari K., Kalanon M., Kuras R., Lefebvre P.A.,
RA   Lemaire S.D., Lobanov A.V., Lohr M., Manuell A., Meier I., Mets L.,
RA   Mittag M., Mittelmeier T., Moroney J.V., Moseley J., Napoli C.,
RA   Nedelcu A.M., Niyogi K., Novoselov S.V., Paulsen I.T., Pazour G.J.,
RA   Purton S., Ral J.P., Riano-Pachon D.M., Riekhof W., Rymarquis L.,
RA   Schroda M., Stern D., Umen J., Willows R., Wilson N., Zimmer S.L.,
RA   Allmer J., Balk J., Bisova K., Chen C.J., Elias M., Gendler K., Hauser C.,
RA   Lamb M.R., Ledford H., Long J.C., Minagawa J., Page M.D., Pan J.,
RA   Pootakham W., Roje S., Rose A., Stahlberg E., Terauchi A.M., Yang P.,
RA   Ball S., Bowler C., Dieckmann C.L., Gladyshev V.N., Green P., Jorgensen R.,
RA   Mayfield S., Mueller-Roeber B., Rajamani S., Sayre R.T., Brokstein P.,
RA   Dubchak I., Goodstein D., Hornick L., Huang Y.W., Jhaveri J., Luo Y.,
RA   Martinez D., Ngau W.C., Otillar B., Poliakov A., Porter A., Szajkowski L.,
RA   Werner G., Zhou K., Grigoriev I.V., Rokhsar D.S., Grossman A.R.;
RT   "The Chlamydomonas genome reveals the evolution of key animal and plant
RT   functions.";
RL   Science 318:245-250(2007).
RN   [2]
RP   SUBCELLULAR LOCATION, AND INDUCTION.
RX   PubMed=15964273; DOI=10.1016/j.cub.2005.05.024;
RA   Keller L.C., Romijn E.P., Zamora I., Yates J.R. III, Marshall W.F.;
RT   "Proteomic analysis of isolated chlamydomonas centrioles reveals orthologs
RT   of ciliary-disease genes.";
RL   Curr. Biol. 15:1090-1098(2005).
RN   [3]
RP   FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY.
RX   PubMed=24259666; DOI=10.1242/jcs.140723;
RA   Meng D., Cao M., Oda T., Pan J.;
RT   "The conserved ciliary protein Bug22 controls planar beating of
RT   Chlamydomonas flagella.";
RL   J. Cell Sci. 127:281-287(2014).
RN   [4]
RP   FUNCTION, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX   PubMed=24574454; DOI=10.1091/mbc.e13-08-0464;
RA   Yanagisawa H.A., Mathis G., Oda T., Hirono M., Richey E.A., Ishikawa H.,
RA   Marshall W.F., Kikkawa M., Qin H.;
RT   "FAP20 is an inner junction protein of doublet microtubules essential for
RT   both the planar asymmetrical waveform and stability of flagella in
RT   Chlamydomonas.";
RL   Mol. Biol. Cell 25:1472-1483(2014).
CC   -!- FUNCTION: Cilium- and flagellum-specific protein that plays a role in
CC       axonemal structure organization and motility (PubMed:24259666,
CC       PubMed:24574454). Involved in the control of flagellar beating in an
CC       asymmetric and planar waveform (PubMed:24259666, PubMed:24574454).
CC       Stabilizes outer doublet microtubules (DMTs) of the axoneme and may
CC       work as a scaffold for intratubular proteins, such as tektin and PACRG,
CC       to produce the beak structures in DMT1, 5 and 6 (PubMed:24259666,
CC       PubMed:24574454). Not essential for flagellar assembly
CC       (PubMed:24574454). {ECO:0000269|PubMed:24259666,
CC       ECO:0000269|PubMed:24574454}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, flagellum axoneme.
CC       Cytoplasm, cytoskeleton, flagellum basal body. Note=Localizes
CC       predominantly along the length of all nine axonemal outer doublet
CC       microtubules (DMTs). Localizes to the inner junction (IJ) between
CC       A- and B-tubules of the DMTs. Associates to the basal bodies in a
CC       microtubule-dependent manner. Enters into the flagella through
CC       diffusion.
CC   -!- INDUCTION: Up-regulated during flagellar assembly.
CC       {ECO:0000269|PubMed:15964273}.
CC   -!- DISRUPTION PHENOTYPE: Exhibit erratic movements with cells continuously
CC       turning around the body axis. Display longer and sharp kinks flagella.
CC       Flagella beat in a uncoordinated and deformed non-planar manner. Lost
CC       beak-like strucure projections in B-tubules of the outer doublet
CC       microtubules (DMTs) 1, 5 and 6. {ECO:0000269|PubMed:24259666,
CC       ECO:0000269|PubMed:24574454}.
CC   -!- SIMILARITY: Belongs to the CFAP20 family. {ECO:0000305}.
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DR   EMBL; DS496123; EDP03967.1; -; Genomic_DNA.
DR   RefSeq; XP_001692489.1; XM_001692437.1.
DR   PDB; 6U42; EM; 3.40 A; 3W/3Y/4A/4C/4E/4G/4I=1-190.
DR   PDB; 6VE7; EM; 3.60 A; 3/c/g/z=1-190.
DR   PDBsum; 6U42; -.
DR   PDBsum; 6VE7; -.
DR   AlphaFoldDB; A8IU92; -.
DR   SMR; A8IU92; -.
DR   STRING; 3055.EDP03967; -.
DR   PaxDb; A8IU92; -.
DR   PRIDE; A8IU92; -.
DR   EnsemblPlants; PNW81334; PNW81334; CHLRE_07g351650v5.
DR   GeneID; 5718073; -.
DR   Gramene; PNW81334; PNW81334; CHLRE_07g351650v5.
DR   KEGG; cre:CHLRE_07g351650v5; -.
DR   eggNOG; KOG3213; Eukaryota.
DR   HOGENOM; CLU_060610_1_1_1; -.
DR   InParanoid; A8IU92; -.
DR   OrthoDB; 1213295at2759; -.
DR   GO; GO:0097540; C:axonemal central pair; IDA:UniProtKB.
DR   GO; GO:0005879; C:axonemal microtubule; IDA:UniProtKB.
DR   GO; GO:0097545; C:axonemal outer doublet; IDA:UniProtKB.
DR   GO; GO:0036064; C:ciliary basal body; IDA:UniProtKB.
DR   GO; GO:0005875; C:microtubule associated complex; IDA:UniProtKB.
DR   GO; GO:0031514; C:motile cilium; IDA:UniProtKB.
DR   GO; GO:0035082; P:axoneme assembly; IDA:UniProtKB.
DR   GO; GO:0060271; P:cilium assembly; IMP:UniProtKB.
DR   GO; GO:0072594; P:establishment of protein localization to organelle; IMP:UniProtKB.
DR   GO; GO:2000155; P:positive regulation of cilium-dependent cell motility; IDA:UniProtKB.
DR   GO; GO:0060296; P:regulation of cilium beat frequency involved in ciliary motility; IMP:UniProtKB.
DR   InterPro; IPR040441; CFA20/CFAP20DC.
DR   InterPro; IPR007714; CFA20_dom.
DR   PANTHER; PTHR12458; PTHR12458; 1.
DR   Pfam; PF05018; DUF667; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cell projection; Cilium; Cytoplasm; Cytoskeleton; Flagellum;
KW   Microtubule.
FT   CHAIN           1..190
FT                   /note="Cilia- and flagella-associated protein 20"
FT                   /id="PRO_0000430499"
SQ   SEQUENCE   190 AA;  22166 MW;  9B02DAEDD820B87A CRC64;
     MFKNAFQSGF LSVLYSIGSK PLEIWDKQVS NGHIKRITDA DIQSSVLEIM GQNVSTTYIT
     CPADPNKTLG IKLPFLVLII KNLNKYFSFE VQVLDDKNVR RRFRASNYQS TTRVKPFICT
     MPMRLDSGWN QIQFNLSDFT RRAYGTNYIE TLRVQVHANC RIRRIYFSDR LYSEEELPAE
     FKLFLPIQKS