ID   CFA20_DANRE             Reviewed;         192 AA.
AC   Q6PBJ2;
DT   24-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 77.
DE   RecName: Full=Cilia- and flagella-associated protein 20;
GN   Name=cfap20; ORFNames=zgc:73380;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Eye;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=24574454; DOI=10.1091/mbc.e13-08-0464;
RA   Yanagisawa H.A., Mathis G., Oda T., Hirono M., Richey E.A., Ishikawa H.,
RA   Marshall W.F., Kikkawa M., Qin H.;
RT   "FAP20 is an inner junction protein of doublet microtubules essential for
RT   both the planar asymmetrical waveform and stability of flagella in
RT   Chlamydomonas.";
RL   Mol. Biol. Cell 25:1472-1483(2014).
CC   -!- FUNCTION: Cilium- and flagellum-specific protein that plays a role in
CC       axonemal structure organization and motility. Involved in the
CC       regulation of the size and morphology of cilia. Required for axonemal
CC       microtubules polyglutamylation (By similarity). Plays a role in cilia
CC       stability (PubMed:24574454). {ECO:0000250|UniProtKB:Q9Y6A4,
CC       ECO:0000269|PubMed:24574454}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9Y6A4}.
CC       Cytoplasm, cytoskeleton, microtubule organizing center, centrosome,
CC       centriole {ECO:0000250|UniProtKB:Q9Y6A4}. Cytoplasm, cytoskeleton,
CC       cilium basal body {ECO:0000250|UniProtKB:Q9Y6A4}. Cytoplasm,
CC       cytoskeleton, cilium axoneme {ECO:0000250|UniProtKB:Q6B857}.
CC       Note=Microtubule inner protein (MIP) part of the dynein-decorated
CC       doublet microtubules (DMTs) in cilia axoneme.
CC       {ECO:0000250|UniProtKB:Q6B857}.
CC   -!- DISRUPTION PHENOTYPE: Display curved body axis, short somite length and
CC       defective heart-looping orientation. {ECO:0000269|PubMed:24574454}.
CC   -!- SIMILARITY: Belongs to the CFAP20 family. {ECO:0000305}.
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DR   EMBL; BC059688; AAH59688.1; -; mRNA.
DR   RefSeq; NP_957105.1; NM_200811.1.
DR   AlphaFoldDB; Q6PBJ2; -.
DR   SMR; Q6PBJ2; -.
DR   STRING; 7955.ENSDARP00000057261; -.
DR   PaxDb; Q6PBJ2; -.
DR   Ensembl; ENSDART00000165516; ENSDARP00000140557; ENSDARG00000100514.
DR   GeneID; 393784; -.
DR   KEGG; dre:393784; -.
DR   CTD; 29105; -.
DR   ZFIN; ZDB-GENE-040426-1784; cfap20.
DR   eggNOG; KOG3213; Eukaryota.
DR   GeneTree; ENSGT00390000004554; -.
DR   InParanoid; Q6PBJ2; -.
DR   OMA; TTYISCP; -.
DR   PhylomeDB; Q6PBJ2; -.
DR   TreeFam; TF313405; -.
DR   PRO; PR:Q6PBJ2; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 19.
DR   Bgee; ENSDARG00000100514; Expressed in testis and 36 other tissues.
DR   ExpressionAtlas; Q6PBJ2; baseline.
DR   GO; GO:0005879; C:axonemal microtubule; ISS:UniProtKB.
DR   GO; GO:0005814; C:centriole; ISS:UniProtKB.
DR   GO; GO:0036064; C:ciliary basal body; ISS:UniProtKB.
DR   GO; GO:0005929; C:cilium; ISS:UniProtKB.
DR   GO; GO:0031514; C:motile cilium; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0060271; P:cilium assembly; IMP:UniProtKB.
DR   GO; GO:2000147; P:positive regulation of cell motility; ISS:UniProtKB.
DR   GO; GO:2000253; P:positive regulation of feeding behavior; ISS:UniProtKB.
DR   GO; GO:0018095; P:protein polyglutamylation; ISS:UniProtKB.
DR   GO; GO:0060296; P:regulation of cilium beat frequency involved in ciliary motility; ISS:UniProtKB.
DR   GO; GO:1901207; P:regulation of heart looping; IMP:UniProtKB.
DR   GO; GO:0014807; P:regulation of somitogenesis; IMP:UniProtKB.
DR   GO; GO:0065001; P:specification of axis polarity; IMP:UniProtKB.
DR   InterPro; IPR040441; CFA20/CFAP20DC.
DR   InterPro; IPR007714; CFA20_dom.
DR   PANTHER; PTHR12458; PTHR12458; 1.
DR   Pfam; PF05018; DUF667; 1.
PE   2: Evidence at transcript level;
KW   Cell projection; Cilium; Cytoplasm; Cytoskeleton; Microtubule; Nucleus;
KW   Reference proteome.
FT   CHAIN           1..192
FT                   /note="Cilia- and flagella-associated protein 20"
FT                   /id="PRO_0000296402"
SQ   SEQUENCE   192 AA;  22606 MW;  5EF27EEA15A91B78 CRC64;
     MFKNTFQSGF LSILYSIGSK PLQIWDKKVR NGHIKRITDN DIQSLVLEVE GTNVSTTYIT
     CPADPKKTLG IKLPFLVMII KNLKKYFTFE VQVLDDKNVR RRFRASNYQS TTRVKPFICT
     MPMRLDDGWN QIQFNLSDFT RRAYGTNYIE TLRVQIHANC RIRRVYFSDR LYSEDELPAE
     FKLYLPVQNK AK