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CFA20_DROME
ID   CFA20_DROME             Reviewed;         199 AA.
AC   Q9VKV8;
DT   24-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   03-AUG-2022, entry version 127.
DE   RecName: Full=Cilia- and flagella-associated protein 20;
DE   AltName: Full=Basal body up-regulated protein 22;
GN   Name=Bug22 {ECO:0000312|FlyBase:FBgn0032248}; Synonyms=CFAP20;
GN   ORFNames=CG5343 {ECO:0000312|FlyBase:FBgn0032248};
OS   Drosophila melanogaster (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7227;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Berkeley;
RX   PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA   Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA   Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA   George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA   Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA   Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA   Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA   An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA   Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA   Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA   Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA   Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA   Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA   Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA   Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA   Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA   Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA   Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA   Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA   Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA   Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA   Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA   McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA   Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA   Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA   Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA   Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA   Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA   Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA   Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA   Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA   Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA   Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA   Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA   Venter J.C.;
RT   "The genome sequence of Drosophila melanogaster.";
RL   Science 287:2185-2195(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=Berkeley;
RX   PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA   Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA   Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA   Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA   Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA   Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA   Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT   "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT   review.";
RL   Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Berkeley; TISSUE=Head;
RX   PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA   Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA   Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA   Celniker S.E.;
RT   "A Drosophila full-length cDNA resource.";
RL   Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN   [4]
RP   FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DISRUPTION
RP   PHENOTYPE.
RX   PubMed=24414207; DOI=10.1242/bio.20146577;
RA   Mendes Maia T., Gogendeau D., Pennetier C., Janke C., Basto R.;
RT   "Bug22 influences cilium morphology and the post-translational modification
RT   of ciliary microtubules.";
RL   Biol. Open 3:138-151(2014).
CC   -!- FUNCTION: Cilium- and flagellum-specific protein that plays a role in
CC       axonemal structure organization and motility. Involved in the
CC       regulation of the size and morphology of cilia. Required for sperm
CC       individualization, differentiation of the sperm flagellum and tubulin
CC       polyglycylation of axonemal microtubules.
CC       {ECO:0000269|PubMed:24414207}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:24414207}. Nucleus,
CC       nucleolus {ECO:0000269|PubMed:24414207}. Cell projection, cilium
CC       {ECO:0000269|PubMed:24414207}. Cytoplasm, cytoskeleton, microtubule
CC       organizing center, centrosome, centriole {ECO:0000269|PubMed:24414207}.
CC       Cell projection, cilium, flagellum {ECO:0000269|PubMed:24414207}.
CC       Note=Localizes in cilia of chordotonal organs in sensory neurons of the
CC       antenna. Localizes in small primary cilium composed of a transition
CC       zone and a short axoneme. Localizes at the top of the giant centrioles
CC       and enriched in the nucleolus of spermatocytes.
CC   -!- TISSUE SPECIFICITY: Expressed in spermatocytes and chordotonal organs
CC       in sensory neurons of the antenna. {ECO:0000269|PubMed:24414207}.
CC   -!- DISRUPTION PHENOTYPE: Flies are viable and develop until late pupal
CC       stages. Display defects in wing posture and inflation, in locomotion
CC       and climbing activities and have a reduced lifespan. Males produce
CC       immotile sperm and show defects in sperm individualization process
CC       within cysts. Display defects of polyglycylation incorporation in sperm
CC       axonemal microtubules. {ECO:0000269|PubMed:24414207}.
CC   -!- SIMILARITY: Belongs to the CFAP20 family. {ECO:0000305}.
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DR   EMBL; AE014134; AAF52950.1; -; Genomic_DNA.
DR   EMBL; AY084152; AAL89890.1; -; mRNA.
DR   RefSeq; NP_609402.1; NM_135558.4.
DR   AlphaFoldDB; Q9VKV8; -.
DR   SMR; Q9VKV8; -.
DR   BioGRID; 60510; 6.
DR   IntAct; Q9VKV8; 1.
DR   STRING; 7227.FBpp0079634; -.
DR   PaxDb; Q9VKV8; -.
DR   PRIDE; Q9VKV8; -.
DR   DNASU; 34429; -.
DR   EnsemblMetazoa; FBtr0080044; FBpp0079634; FBgn0032248.
DR   GeneID; 34429; -.
DR   KEGG; dme:Dmel_CG5343; -.
DR   UCSC; CG5343-RA; d. melanogaster.
DR   CTD; 34429; -.
DR   FlyBase; FBgn0032248; Bug22.
DR   VEuPathDB; VectorBase:FBgn0032248; -.
DR   eggNOG; KOG3213; Eukaryota.
DR   GeneTree; ENSGT00390000004554; -.
DR   HOGENOM; CLU_060610_1_1_1; -.
DR   InParanoid; Q9VKV8; -.
DR   OMA; TTYISCP; -.
DR   OrthoDB; 1213295at2759; -.
DR   PhylomeDB; Q9VKV8; -.
DR   BioGRID-ORCS; 34429; 1 hit in 1 CRISPR screen.
DR   GenomeRNAi; 34429; -.
DR   PRO; PR:Q9VKV8; -.
DR   Proteomes; UP000000803; Chromosome 2L.
DR   Bgee; FBgn0032248; Expressed in testis and 27 other tissues.
DR   Genevisible; Q9VKV8; DM.
DR   GO; GO:0005879; C:axonemal microtubule; ISS:UniProtKB.
DR   GO; GO:0071013; C:catalytic step 2 spliceosome; HDA:FlyBase.
DR   GO; GO:0005814; C:centriole; IEA:UniProtKB-SubCell.
DR   GO; GO:0036064; C:ciliary basal body; IBA:GO_Central.
DR   GO; GO:0005929; C:cilium; IDA:FlyBase.
DR   GO; GO:0031514; C:motile cilium; IBA:GO_Central.
DR   GO; GO:0005730; C:nucleolus; IDA:FlyBase.
DR   GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR   GO; GO:0071011; C:precatalytic spliceosome; HDA:FlyBase.
DR   GO; GO:0036126; C:sperm flagellum; IDA:FlyBase.
DR   GO; GO:0060271; P:cilium assembly; IMP:UniProtKB.
DR   GO; GO:0044782; P:cilium organization; IMP:FlyBase.
DR   GO; GO:0040011; P:locomotion; IMP:FlyBase.
DR   GO; GO:0000398; P:mRNA splicing, via spliceosome; IC:FlyBase.
DR   GO; GO:2000147; P:positive regulation of cell motility; IBA:GO_Central.
DR   GO; GO:1902093; P:positive regulation of flagellated sperm motility; IMP:UniProtKB.
DR   GO; GO:0018094; P:protein polyglycylation; IMP:UniProtKB.
DR   GO; GO:0060296; P:regulation of cilium beat frequency involved in ciliary motility; IBA:GO_Central.
DR   GO; GO:0007288; P:sperm axoneme assembly; IMP:UniProtKB.
DR   GO; GO:0007291; P:sperm individualization; IMP:FlyBase.
DR   InterPro; IPR040441; CFA20/CFAP20DC.
DR   InterPro; IPR007714; CFA20_dom.
DR   PANTHER; PTHR12458; PTHR12458; 1.
DR   Pfam; PF05018; DUF667; 1.
PE   2: Evidence at transcript level;
KW   Cell projection; Cilium; Cytoplasm; Cytoskeleton; Differentiation;
KW   Flagellum; Microtubule; Nucleus; Reference proteome; Spermatogenesis.
FT   CHAIN           1..199
FT                   /note="Cilia- and flagella-associated protein 20"
FT                   /id="PRO_0000296407"
SQ   SEQUENCE   199 AA;  23331 MW;  58B216FDF52A0C78 CRC64;
     MFKNTFQSGF LSILYSIGSK PLQLWDKKVR NGHIKRITDN DIQSLVLEIV GTNVSTTFIT
     CPADPKKTLG IKLPFLVMII KNMKKYFTFE VQVLDDKNVR RRFRASNYQS TTRVKPFICT
     MPMRLDEGWN QIQFNLSDFT RRAYGTNYVE TLRVQIHANC RIRRVYFSDR LYSEDELPPE
     FKLFLPIQKP VQKSNAICS
 
 
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