CFA20_MOUSE
ID CFA20_MOUSE Reviewed; 193 AA.
AC Q8BTU1; P70212; Q3T9D6;
DT 24-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Cilia- and flagella-associated protein 20;
DE AltName: Full=Gene trap locus 3 protein;
GN Name=Cfap20; Synonyms=Gtl3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RC STRAIN=BALB/cJ; TISSUE=Testis;
RX PubMed=8688464; DOI=10.1016/0167-4781(96)00008-5;
RA Ruether U., Rijkers T.;
RT "Sequence and expression pattern of an evolutionarily conserved transcript
RT identified by gene trapping.";
RL Biochim. Biophys. Acta 1307:294-300(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=NOD; TISSUE=Thymus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP DEVELOPMENTAL STAGE.
RX PubMed=15803458; DOI=10.1002/mrd.20282;
RA Hwang K.-C., Park S.-Y., Park S.-P., Lim J.H., Cui X.-S., Kim N.-H.;
RT "Specific maternal transcripts in bovine oocytes and cleavaged embryos:
RT identification with novel DDRT-PCR methods.";
RL Mol. Reprod. Dev. 71:275-283(2005).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Kidney, Lung, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Cilium- and flagellum-specific protein that plays a role in
CC axonemal structure organization and motility (By similarity). Involved
CC in the regulation of the size and morphology of cilia. Required for
CC axonemal microtubules polyglutamylation (By similarity).
CC {ECO:0000250|UniProtKB:Q6B857, ECO:0000250|UniProtKB:Q9Y6A4}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9Y6A4}.
CC Cytoplasm, cytoskeleton, microtubule organizing center, centrosome,
CC centriole {ECO:0000250|UniProtKB:Q9Y6A4}. Cytoplasm, cytoskeleton,
CC cilium basal body {ECO:0000250|UniProtKB:Q9Y6A4}. Cytoplasm,
CC cytoskeleton, cilium axoneme {ECO:0000250|UniProtKB:Q6B857}.
CC Note=Microtubule inner protein (MIP) part of the dynein-decorated
CC doublet microtubules (DMTs) in cilia axoneme.
CC {ECO:0000250|UniProtKB:Q6B857}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8BTU1-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8BTU1-2; Sequence=VSP_027219;
CC -!- TISSUE SPECIFICITY: Widely expressed, with highest levels in testis and
CC lowest in muscle. {ECO:0000269|PubMed:8688464}.
CC -!- DEVELOPMENTAL STAGE: Expressed at high levels in germinal vesicle stage
CC oocytes at the mRNA level. Expression progressively decreases until
CC blastocyst stage (PubMed:15803458). Detected in later embryonic stages
CC from 9.5 to 19.5 dpc. At 11.5 dpc, the expression is widespread, with
CC no preference for any particular organ or structure (PubMed:8688464).
CC {ECO:0000269|PubMed:15803458, ECO:0000269|PubMed:8688464}.
CC -!- SIMILARITY: Belongs to the CFAP20 family. {ECO:0000305}.
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DR EMBL; Z54179; CAA90889.1; -; mRNA.
DR EMBL; AK088713; BAC40521.1; -; mRNA.
DR EMBL; AK172603; BAE43087.1; -; mRNA.
DR EMBL; BC119023; AAI19024.1; -; mRNA.
DR EMBL; BC119025; AAI19026.1; -; mRNA.
DR CCDS; CCDS40445.1; -. [Q8BTU1-1]
DR RefSeq; NP_032213.2; NM_008187.2. [Q8BTU1-1]
DR AlphaFoldDB; Q8BTU1; -.
DR SMR; Q8BTU1; -.
DR BioGRID; 200114; 1.
DR STRING; 10090.ENSMUSP00000034249; -.
DR iPTMnet; Q8BTU1; -.
DR PhosphoSitePlus; Q8BTU1; -.
DR EPD; Q8BTU1; -.
DR MaxQB; Q8BTU1; -.
DR PaxDb; Q8BTU1; -.
DR PeptideAtlas; Q8BTU1; -.
DR PRIDE; Q8BTU1; -.
DR ProteomicsDB; 281390; -. [Q8BTU1-1]
DR ProteomicsDB; 281391; -. [Q8BTU1-2]
DR Antibodypedia; 15216; 119 antibodies from 23 providers.
DR DNASU; 14894; -.
DR Ensembl; ENSMUST00000034249; ENSMUSP00000034249; ENSMUSG00000031796. [Q8BTU1-1]
DR Ensembl; ENSMUST00000213086; ENSMUSP00000148651; ENSMUSG00000031796. [Q8BTU1-2]
DR GeneID; 14894; -.
DR KEGG; mmu:14894; -.
DR UCSC; uc009myi.2; mouse. [Q8BTU1-2]
DR UCSC; uc009myj.2; mouse. [Q8BTU1-1]
DR CTD; 29105; -.
DR MGI; MGI:107428; Cfap20.
DR VEuPathDB; HostDB:ENSMUSG00000031796; -.
DR eggNOG; KOG3213; Eukaryota.
DR GeneTree; ENSGT00390000004554; -.
DR HOGENOM; CLU_060610_1_1_1; -.
DR InParanoid; Q8BTU1; -.
DR OMA; TTYISCP; -.
DR OrthoDB; 1213295at2759; -.
DR PhylomeDB; Q8BTU1; -.
DR TreeFam; TF313405; -.
DR BioGRID-ORCS; 14894; 28 hits in 72 CRISPR screens.
DR ChiTaRS; Cfap20; mouse.
DR PRO; PR:Q8BTU1; -.
DR Proteomes; UP000000589; Chromosome 8.
DR RNAct; Q8BTU1; protein.
DR Bgee; ENSMUSG00000031796; Expressed in olfactory epithelium and 269 other tissues.
DR ExpressionAtlas; Q8BTU1; baseline and differential.
DR Genevisible; Q8BTU1; MM.
DR GO; GO:0005879; C:axonemal microtubule; ISS:UniProtKB.
DR GO; GO:0005814; C:centriole; ISS:UniProtKB.
DR GO; GO:0036064; C:ciliary basal body; ISS:UniProtKB.
DR GO; GO:0005929; C:cilium; IDA:MGI.
DR GO; GO:0031514; C:motile cilium; IBA:GO_Central.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0060271; P:cilium assembly; ISS:UniProtKB.
DR GO; GO:2000147; P:positive regulation of cell motility; ISS:UniProtKB.
DR GO; GO:2000253; P:positive regulation of feeding behavior; ISS:UniProtKB.
DR GO; GO:0018095; P:protein polyglutamylation; ISS:UniProtKB.
DR GO; GO:0060296; P:regulation of cilium beat frequency involved in ciliary motility; ISS:UniProtKB.
DR InterPro; IPR040441; CFA20/CFAP20DC.
DR InterPro; IPR007714; CFA20_dom.
DR PANTHER; PTHR12458; PTHR12458; 1.
DR Pfam; PF05018; DUF667; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell projection; Cilium; Cytoplasm; Cytoskeleton;
KW Microtubule; Nucleus; Reference proteome.
FT CHAIN 1..193
FT /note="Cilia- and flagella-associated protein 20"
FT /id="PRO_0000296399"
FT VAR_SEQ 156..193
FT /note="IHANCRIRRVYFSDRLYSEDELPAEFKLYLPVQNKAKQ -> VLHLPHRESH
FT TGGFRTLWLANWLAYGPWSRTM (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_027219"
FT CONFLICT 72
FT /note="K -> I (in Ref. 1; CAA90889)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 193 AA; 22748 MW; 9DF3DB123A15BF77 CRC64;
MFKNTFQSGF LSILYSIGSK PLQIWDKKVR NGHIKRITDN DIQSLVLEIE GTNVSTTYIT
CPADPKKTLG IKLPFLVMII KNLKKYFTFE VQVLDDKNVR RRFRASNYQS TTRVKPFICT
MPMRLDDGWN QIQFNLSDFT RRAYGTNYIE TLRVQIHANC RIRRVYFSDR LYSEDELPAE
FKLYLPVQNK AKQ
