CFA43_TRYB2
ID CFA43_TRYB2 Reviewed; 1460 AA.
AC Q580P9; D6XEU2;
DT 10-OCT-2018, integrated into UniProtKB/Swiss-Prot.
DT 10-MAY-2005, sequence version 1.
DT 25-MAY-2022, entry version 84.
DE RecName: Full=Cilia- and flagella-associated protein 43 {ECO:0000303|PubMed:29449551};
GN Name=CFAP43 {ECO:0000303|PubMed:29449551}; ORFNames=Tb927.4.5380;
OS Trypanosoma brucei brucei (strain 927/4 GUTat10.1).
OC Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina;
OC Trypanosomatida; Trypanosomatidae; Trypanosoma.
OX NCBI_TaxID=185431;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=927/4 GUTat10.1;
RX PubMed=16020726; DOI=10.1126/science.1112642;
RA Berriman M., Ghedin E., Hertz-Fowler C., Blandin G., Renauld H.,
RA Bartholomeu D.C., Lennard N.J., Caler E., Hamlin N.E., Haas B., Bohme U.,
RA Hannick L., Aslett M.A., Shallom J., Marcello L., Hou L., Wickstead B.,
RA Alsmark U.C.M., Arrowsmith C., Atkin R.J., Barron A.J., Bringaud F.,
RA Brooks K., Carrington M., Cherevach I., Chillingworth T.J., Churcher C.,
RA Clark L.N., Corton C.H., Cronin A., Davies R.M., Doggett J., Djikeng A.,
RA Feldblyum T., Field M.C., Fraser A., Goodhead I., Hance Z., Harper D.,
RA Harris B.R., Hauser H., Hostetler J., Ivens A., Jagels K., Johnson D.,
RA Johnson J., Jones K., Kerhornou A.X., Koo H., Larke N., Landfear S.,
RA Larkin C., Leech V., Line A., Lord A., Macleod A., Mooney P.J., Moule S.,
RA Martin D.M., Morgan G.W., Mungall K., Norbertczak H., Ormond D., Pai G.,
RA Peacock C.S., Peterson J., Quail M.A., Rabbinowitsch E., Rajandream M.A.,
RA Reitter C., Salzberg S.L., Sanders M., Schobel S., Sharp S., Simmonds M.,
RA Simpson A.J., Tallon L., Turner C.M., Tait A., Tivey A.R., Van Aken S.,
RA Walker D., Wanless D., Wang S., White B., White O., Whitehead S.,
RA Woodward J., Wortman J., Adams M.D., Embley T.M., Gull K., Ullu E.,
RA Barry J.D., Fairlamb A.H., Opperdoes F., Barrell B.G., Donelson J.E.,
RA Hall N., Fraser C.M., Melville S.E., El-Sayed N.M.A.;
RT "The genome of the African trypanosome Trypanosoma brucei.";
RL Science 309:416-422(2005).
RN [2]
RP SUBCELLULAR LOCATION, AND FUNCTION.
RX PubMed=29449551; DOI=10.1038/s41467-017-02792-7;
RA Coutton C., Vargas A.S., Amiri-Yekta A., Kherraf Z.E., Ben Mustapha S.F.,
RA Le Tanno P., Wambergue-Legrand C., Karaouzene T., Martinez G., Crouzy S.,
RA Daneshipour A., Hosseini S.H., Mitchell V., Halouani L., Marrakchi O.,
RA Makni M., Latrous H., Kharouf M., Deleuze J.F., Boland A., Hennebicq S.,
RA Satre V., Jouk P.S., Thierry-Mieg N., Conne B., Dacheux D., Landrein N.,
RA Schmitt A., Stouvenel L., Lores P., El Khouri E., Bottari S.P., Faure J.,
RA Wolf J.P., Pernet-Gallay K., Escoffier J., Gourabi H., Robinson D.R.,
RA Nef S., Dulioust E., Zouari R., Bonhivers M., Toure A., Arnoult C.,
RA Ray P.F.;
RT "Mutations in CFAP43 and CFAP44 cause male infertility and flagellum
RT defects in Trypanosoma and human.";
RL Nat. Commun. 9:686-686(2018).
CC -!- FUNCTION: Flagellar protein involved in flagellum axoneme organization
CC and function. {ECO:0000269|PubMed:29449551}.
CC -!- SUBCELLULAR LOCATION: Cell projection, cilium, flagellum
CC {ECO:0000269|PubMed:29449551}. Cytoplasm, cytoskeleton, flagellum
CC axoneme {ECO:0000269|PubMed:29449551}. Note=Closely associated to the
CC axoneme along the paraflagellar rod. {ECO:0000269|PubMed:29449551}.
CC -!- SIMILARITY: Belongs to the CFAP43 family. {ECO:0000305}.
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DR EMBL; AC096672; AAX79135.1; -; Genomic_DNA.
DR EMBL; CP000067; AAZ11125.1; -; Genomic_DNA.
DR RefSeq; XP_844684.1; XM_839591.1.
DR AlphaFoldDB; Q580P9; -.
DR STRING; 5691.AAZ11125; -.
DR PaxDb; Q580P9; -.
DR GeneID; 3657075; -.
DR KEGG; tbr:Tb927.4.5380; -.
DR VEuPathDB; TriTrypDB:Tb927.4.5380; -.
DR eggNOG; ENOG502QQ39; Eukaryota.
DR InParanoid; Q580P9; -.
DR OMA; WMDGLEK; -.
DR Proteomes; UP000008524; Chromosome 4.
DR GO; GO:0005930; C:axoneme; IDA:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; IDA:GeneDB.
DR GO; GO:0031514; C:motile cilium; IEA:UniProtKB-SubCell.
DR GO; GO:0035082; P:axoneme assembly; IMP:GeneDB.
DR GO; GO:0060271; P:cilium assembly; IBA:GO_Central.
DR GO; GO:0060285; P:cilium-dependent cell motility; IMP:UniProtKB.
DR GO; GO:0007283; P:spermatogenesis; IMP:GeneDB.
DR Gene3D; 2.130.10.10; -; 2.
DR InterPro; IPR039692; CFAP43.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR14885:SF1; PTHR14885:SF1; 1.
DR SUPFAM; SSF50978; SSF50978; 1.
PE 3: Inferred from homology;
KW Cell projection; Cilium; Coiled coil; Cytoplasm; Cytoskeleton; Flagellum;
KW Reference proteome; Repeat; WD repeat.
FT CHAIN 1..1460
FT /note="Cilia- and flagella-associated protein 43"
FT /id="PRO_0000445513"
FT REPEAT 46..87
FT /note="WD 1"
FT /evidence="ECO:0000255"
FT REPEAT 91..132
FT /note="WD 2"
FT /evidence="ECO:0000255"
FT REPEAT 184..221
FT /note="WD 3"
FT /evidence="ECO:0000255"
FT REPEAT 303..342
FT /note="WD 4"
FT /evidence="ECO:0000255"
FT REPEAT 428..468
FT /note="WD 5"
FT /evidence="ECO:0000255"
FT REPEAT 529..569
FT /note="WD 6"
FT /evidence="ECO:0000255"
FT REPEAT 589..628
FT /note="WD 7"
FT /evidence="ECO:0000255"
FT REPEAT 911..951
FT /note="WD 8"
FT /evidence="ECO:0000255"
FT REPEAT 1129..1170
FT /note="WD 9"
FT /evidence="ECO:0000255"
FT COILED 1170..1214
FT /evidence="ECO:0000255"
FT COILED 1399..1446
FT /evidence="ECO:0000255"
SQ SEQUENCE 1460 AA; 164952 MW; 1A8FA1943A7DAB98 CRC64;
MATNARILGF NSPSALVAAG DDFLLGAGGG IVIRRKEESS QWIPCEGRYA IGALAFSPSA
GLLCVTEVKL DVSLHVFRFP ERHHLQCIDN VATVDVQHML FSSDGEMLAL LTCIPTTCVT
FYSAARGNRL VKCASTELGG VFCKHLTFPL HRHDCIAVLE PHGVRIACNM DSATFVPSIL
TLSSKGHYFH SCVWGTEGLY CGAGRGQVVL LDELRTDMKN YINCETPHNV TALLQNGTLL
FIGTECGDVF TYNIDQKAQR LLVRLGRSVV RLLTLPDVND VLVATSTDVT KISVDTAQSV
FVRRRSASDT VKLLVLGGLV VIVCLDGSLV TYDQDTNTAG HTPVRFPEKV VDACVVGSVA
VVVYDSGFVR SFTVENTVSV VSQMKVSDCP LTACTSDGVS LLAVCDKNVV HFIEVADGLL
ETAASSDIFA CAVTNLRWAV NGGRSVLAAC NNGEVHNLRF TGKCDSASAG VTVDMTWRLD
FPVNDFLPLY GDGDVINIFV HSVDKDTKMY ALERQRVKES KPLRPYFLMR DHECGGNVLQ
RLGGDSIISA GGDGRVVVRD ISHYLMKLPP VPPTKEKKHP LKEFLLRPFG RGGITCLSVW
NAAGGFVCGG NDSVVHLVPV GKSPIHYSWS EPFWHQRAIS TSPSRASSPS DAETLSAERS
RCRIISALAD LRMEVEKLLQ ERTPTVRAED FLLPEQRQAF NEECEMEIHK AREDDYYSLV
HNEFVQHTIK TECWDVMEVQ RSKIVSMTDP ETEVHNFHLR KPCAQRAKIQ KKIKLMRAIQ
IKTEECFTLS SLVKRAKEGN LCTEQQVCGP PSDVDELLYD TLDVYTGPRA TIQLILLECK
ILHEKKSFNI RFDTLRERKS RELNLIAERN GRCVRIMQQL GEHTCPPNVL FTPVFDIEED
PQTVFEVFDS EIDPELLKLA VKSDDGELVV SPSDEAALKT WMDGLEKVTE VLRVNVPIPP
FADNSLEQYV PPEERSDEQQ RIFEEYEKEV AEQTVLINEK KELLRGEVAA LVKANMTSAK
AIDDEIDVLR TDRMLVAQLV DELELHQVNA LCLFLLKKTI RNKFLGVKRE EEDLLCRLRQ
LDSLYEYRLK LYLASEARVQ DCIEEEKNMI TDMRCLPPFT DPDWGERLNR RFTTWRSKYE
DGLAKVPEPT RSGVVPIPLW EQYCQCCRAV VEARDKIIHL RGEADALNDE VVEVETEKKK
AQFALDDKEK AEEACRKEVI EKVLDIQNLY TLQQGQVQDE NAMVSDDFTD FSIRWVKNIT
DYNDLIFASF DEIRSLMSRS SQLRQSMKTC SWETERLLYC IGTLEMELRQ LHTLRVTRQM
QETIHTGAVT SLEREINKMD ARIEAVRSVM SKKVEERNRV ISKLKMQIND RRAENQYLNN
QVQALTNSVE DKKAVWGMLG EHNNDKDRLR ERMRELYENS ELEELARCQQ EELVRLKNEV
DRLREATFPS FAVVTRRTAR
