ID   CFA43_XENLA             Reviewed;        1621 AA.
AC   A0A1L8GXY4;
DT   07-OCT-2020, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2017, sequence version 1.
DT   25-MAY-2022, entry version 18.
DE   RecName: Full=Cilia- and flagella-associated protein 43;
GN   Name=cfap43;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=J;
RX   PubMed=27762356; DOI=10.1038/nature19840;
RA   Session A.M., Uno Y., Kwon T., Chapman J.A., Toyoda A., Takahashi S.,
RA   Fukui A., Hikosaka A., Suzuki A., Kondo M., van Heeringen S.J., Quigley I.,
RA   Heinz S., Ogino H., Ochi H., Hellsten U., Lyons J.B., Simakov O.,
RA   Putnam N., Stites J., Kuroki Y., Tanaka T., Michiue T., Watanabe M.,
RA   Bogdanovic O., Lister R., Georgiou G., Paranjpe S.S., van Kruijsbergen I.,
RA   Shu S., Carlson J., Kinoshita T., Ohta Y., Mawaribuchi S., Jenkins J.,
RA   Grimwood J., Schmutz J., Mitros T., Mozaffari S.V., Suzuki Y., Haramoto Y.,
RA   Yamamoto T.S., Takagi C., Heald R., Miller K., Haudenschild C., Kitzman J.,
RA   Nakayama T., Izutsu Y., Robert J., Fortriede J., Burns K., Lotay V.,
RA   Karimi K., Yasuoka Y., Dichmann D.S., Flajnik M.F., Houston D.W.,
RA   Shendure J., DuPasquier L., Vize P.D., Zorn A.M., Ito M., Marcotte E.M.,
RA   Wallingford J.B., Ito Y., Asashima M., Ueno N., Matsuda Y., Veenstra G.J.,
RA   Fujiyama A., Harland R.M., Taira M., Rokhsar D.S.;
RT   "Genome evolution in the allotetraploid frog Xenopus laevis.";
RL   Nature 538:336-343(2016).
RN   [2]
RP   FUNCTION, DISRUPTION PHENOTYPE, AND DEVELOPMENTAL STAGE.
RX   PubMed=31884020; DOI=10.1016/j.ydbio.2019.12.010;
RA   Rachev E., Schuster-Gossler K., Fuhl F., Ott T., Tveriakhina L.,
RA   Beckers A., Hegermann J., Boldt K., Mai M., Kremmer E., Ueffing M.,
RA   Blum M., Gossler A.;
RT   "CFAP43 modulates ciliary beating in mouse and Xenopus.";
RL   Dev. Biol. 459:109-125(2020).
CC   -!- FUNCTION: Involved in the regulation of the beating frequency of motile
CC       cilia in multiciliated cells of the larval epidermis.
CC       {ECO:0000269|PubMed:31884020}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, cilium axoneme
CC       {ECO:0000250|UniProtKB:E9Q7R9}.
CC   -!- DEVELOPMENTAL STAGE: Expressed in the left-right organizer and floor
CC       plate at embryonic stage 19 (ES19) (PubMed:31884020). Expressed in
CC       multiciliated cells of the larval epidermis and nephrostomes at ES33
CC       and in dorsal lining of the branchial chamber and stomach at ES45
CC       (PubMed:31884020). {ECO:0000269|PubMed:31884020}.
CC   -!- DISRUPTION PHENOTYPE: Morpholino knockdown of the protein results in
CC       embryos with impaired function of motile cilia of the larval epidermis
CC       and majority of embryos develop edema around the heart.
CC       {ECO:0000269|PubMed:31884020}.
CC   -!- SIMILARITY: Belongs to the CFAP43 family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CM004470; OCT88656.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1L8GXY4; -.
DR   STRING; 8355.A0A1L8GXY4; -.
DR   OMA; LLNMDQF; -.
DR   Proteomes; UP000186698; Genome assembly.
DR   GO; GO:0042995; C:cell projection; IEA:UniProtKB-KW.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0005856; C:cytoskeleton; IEA:UniProtKB-KW.
DR   GO; GO:0005576; C:extracellular region; IEA:GOC.
DR   GO; GO:0003351; P:epithelial cilium movement involved in extracellular fluid movement; IMP:MGI.
DR   GO; GO:0003356; P:regulation of cilium beat frequency; IMP:UniProtKB.
DR   Gene3D; 2.130.10.10; -; 4.
DR   InterPro; IPR039692; CFAP43.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   PANTHER; PTHR14885:SF1; PTHR14885:SF1; 2.
DR   SMART; SM00320; WD40; 7.
DR   SUPFAM; SSF50978; SSF50978; 2.
PE   2: Evidence at transcript level;
KW   Cell projection; Coiled coil; Cytoplasm; Cytoskeleton; Reference proteome;
KW   Repeat; WD repeat.
FT   CHAIN           1..1621
FT                   /note="Cilia- and flagella-associated protein 43"
FT                   /id="PRO_0000451142"
FT   REPEAT          97..138
FT                   /note="WD 1"
FT                   /evidence="ECO:0000255"
FT   REPEAT          141..180
FT                   /note="WD 2"
FT                   /evidence="ECO:0000255"
FT   REPEAT          293..330
FT                   /note="WD 3"
FT                   /evidence="ECO:0000255"
FT   REPEAT          334..373
FT                   /note="WD 4"
FT                   /evidence="ECO:0000255"
FT   REPEAT          377..417
FT                   /note="WD 5"
FT                   /evidence="ECO:0000255"
FT   REPEAT          464..503
FT                   /note="WD 6"
FT                   /evidence="ECO:0000255"
FT   REPEAT          634..673
FT                   /note="WD 7"
FT                   /evidence="ECO:0000255"
FT   REPEAT          679..721
FT                   /note="WD 8"
FT                   /evidence="ECO:0000255"
FT   COILED          792..812
FT                   /evidence="ECO:0000255"
FT   COILED          850..870
FT                   /evidence="ECO:0000255"
FT   COILED          1026..1046
FT                   /evidence="ECO:0000255"
FT   COILED          1098..1118
FT                   /evidence="ECO:0000255"
FT   COILED          1150..1177
FT                   /evidence="ECO:0000255"
FT   COILED          1362..1389
FT                   /evidence="ECO:0000255"
FT   COILED          1451..1514
FT                   /evidence="ECO:0000255"
FT   COILED          1591..1611
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   1621 AA;  185118 MW;  A796BF6BB987D930 CRC64;
     MENSGCALEV RWVQGINKKK VTFINDHTVC YPCGNFIVFH DTNTGKWSFL QCTTGSTGAF
     AVNTYSEVVA FSDQKLHPTI YVYTFPGFVK RAELKDGAQL DYSLIAFSHA APYLASYSSI
     PDHVLTIWNW QESIPLCSKS DSQTTYTTLT FNPMNWHQLC LSSERSLTVW NIEICDNQYQ
     LKAMPVKLPS EDGTTDIEEE NLNSHTSNSV SYYGPLMPTS AVAGLVGDEA EIFIPKEQRK
     HSVQPSFHCW NATSDLYVGC AGGQILSISA ETQKVTILAQ KDQSADHLSR TTLLEGNIKT
     MAFHKEGLYV AGNDGVLHLF TIKGSEVKLE DSWNAQEPID SISFSPSYKT LSITTSKGSL
     YLYNQRNPEE TYKLLNVYSR DLLAADFLTF GNKYCLGTDI SGHVQLWSVE DGKSVSSLNL
     NIQATAMACC PSSNYAAVGS STGHIYFIDA VKTEAPRIVQ RTRLYCVPVQ HMHFDPRGNF
     LLTGAADRHI FILDARPSYS FQVLGYIVVC GEILTLSSLS SADTQQTKVM ALVCPVDERK
     EEKGGTQLEM FSLPLQMLSS PSEYIDERGM FKDTMIQKIS YDVDQPLFSA VMGYNSSSVF
     GYSSNETILL KYVIHKDISG SPVTALVAEK VVRGSQLGPG VLCLSPHLKW MAVSGRDGIV
     YVKDLLNMET MAQLQCHSYH NGGINSLAFS LDGQSIVTTG TCDGALVCLQ WKSSGRSSSR
     LGLAGEYGKD LALSLQAAMK EEDQALSRMP VWTADADSLG VERKGKEYSQ LSVDVTEQDS
     FPNTSANDVT WIKRKLEEAE KKETEKYAAE KEVIKTGIKK LRKTIQAMMR ENESLPDIEK
     LDQQEFNLDT EEQERLYLDS EKEVARVRQE IELENLSKQY LREVIKQECW DSMAVKGRSV
     TAFHTGYEVM NYPMKERTPK ELEKFARVLN LMKIEAVDLK VRKEIVETQP KIGPDDEEEV
     EEELMKCQDS SSLVGSLSDH YGGDTSCLYS QLILHSREEK INQIILLQDI IQNIKNAFNK
     DFDTVCRQKE QEITRVMERN NRIQEIMVEL NLQEKLLEPT FTDNEKPERA LTVDDSEVKV
     ERYLTPEQKA KAEHLAKVEE AKRLAAQEDN AKQRALDDMM GGVLEVKKED ILRMEVPQPV
     FLARTEAEWT EEEKKQFKEY EKKCKDLSEE KEKYSKEELK INNLLFSLLI EEEINTRVAH
     LAYILDKKRK QKNQTAEIVK SFKSQVMAFR ESYDNLVAED KLLDRGFKKE FSDITSYQVD
     QLYKLYKRRP RVQRLRTQAD STAPFGERPG SAKAYKDSIA LLMHAMDELD TPENVPEGVE
     LPVWQRFCLA RRSKIEYEQQ VKIKALVLAE MQAFLDKRIE EDEKMRQDIE NFMQELNVLR
     NEKMKFQLDL TVQFLLKQGQ VELENTDLIP SFEDAILLHR SVIEDLNSTI KGLGEQKIAS
     MVESKDFRKG IFQLEWEHRK IRMEMEDLEK KSRDISLLHV SKEFQVFLNE QNYDKRMSDQ
     IQVLEETINA QEKQHGKNVK TYKKILKDLE THINKKIIAN LELDKDLQEL LVSFSERKHI
     YDVVGVEQST EKAAKERYME IIQRRKLVDL AKIQAQEIHE LRSEVDRLRM KTFPALVQME
     Y