CFA44_TRYB2
ID CFA44_TRYB2 Reviewed; 1760 AA.
AC Q57WH1; D6XIN7;
DT 10-OCT-2018, integrated into UniProtKB/Swiss-Prot.
DT 10-MAY-2005, sequence version 1.
DT 25-MAY-2022, entry version 117.
DE RecName: Full=Cilia- and flagella-associated protein 44 {ECO:0000303|PubMed:29449551};
DE AltName: Full=Component of motile flagella 7;
GN Name=CFAP44 {ECO:0000303|PubMed:29449551}; ORFNames=Tb927.7.3560;
OS Trypanosoma brucei brucei (strain 927/4 GUTat10.1).
OC Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina;
OC Trypanosomatida; Trypanosomatidae; Trypanosoma.
OX NCBI_TaxID=185431;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=927/4 GUTat10.1;
RX PubMed=16020726; DOI=10.1126/science.1112642;
RA Berriman M., Ghedin E., Hertz-Fowler C., Blandin G., Renauld H.,
RA Bartholomeu D.C., Lennard N.J., Caler E., Hamlin N.E., Haas B., Bohme U.,
RA Hannick L., Aslett M.A., Shallom J., Marcello L., Hou L., Wickstead B.,
RA Alsmark U.C.M., Arrowsmith C., Atkin R.J., Barron A.J., Bringaud F.,
RA Brooks K., Carrington M., Cherevach I., Chillingworth T.J., Churcher C.,
RA Clark L.N., Corton C.H., Cronin A., Davies R.M., Doggett J., Djikeng A.,
RA Feldblyum T., Field M.C., Fraser A., Goodhead I., Hance Z., Harper D.,
RA Harris B.R., Hauser H., Hostetler J., Ivens A., Jagels K., Johnson D.,
RA Johnson J., Jones K., Kerhornou A.X., Koo H., Larke N., Landfear S.,
RA Larkin C., Leech V., Line A., Lord A., Macleod A., Mooney P.J., Moule S.,
RA Martin D.M., Morgan G.W., Mungall K., Norbertczak H., Ormond D., Pai G.,
RA Peacock C.S., Peterson J., Quail M.A., Rabbinowitsch E., Rajandream M.A.,
RA Reitter C., Salzberg S.L., Sanders M., Schobel S., Sharp S., Simmonds M.,
RA Simpson A.J., Tallon L., Turner C.M., Tait A., Tivey A.R., Van Aken S.,
RA Walker D., Wanless D., Wang S., White B., White O., Whitehead S.,
RA Woodward J., Wortman J., Adams M.D., Embley T.M., Gull K., Ullu E.,
RA Barry J.D., Fairlamb A.H., Opperdoes F., Barrell B.G., Donelson J.E.,
RA Hall N., Fraser C.M., Melville S.E., El-Sayed N.M.A.;
RT "The genome of the African trypanosome Trypanosoma brucei.";
RL Science 309:416-422(2005).
RN [2]
RP SUBCELLULAR LOCATION, AND FUNCTION.
RX PubMed=29449551; DOI=10.1038/s41467-017-02792-7;
RA Coutton C., Vargas A.S., Amiri-Yekta A., Kherraf Z.E., Ben Mustapha S.F.,
RA Le Tanno P., Wambergue-Legrand C., Karaouzene T., Martinez G., Crouzy S.,
RA Daneshipour A., Hosseini S.H., Mitchell V., Halouani L., Marrakchi O.,
RA Makni M., Latrous H., Kharouf M., Deleuze J.F., Boland A., Hennebicq S.,
RA Satre V., Jouk P.S., Thierry-Mieg N., Conne B., Dacheux D., Landrein N.,
RA Schmitt A., Stouvenel L., Lores P., El Khouri E., Bottari S.P., Faure J.,
RA Wolf J.P., Pernet-Gallay K., Escoffier J., Gourabi H., Robinson D.R.,
RA Nef S., Dulioust E., Zouari R., Bonhivers M., Toure A., Arnoult C.,
RA Ray P.F.;
RT "Mutations in CFAP43 and CFAP44 cause male infertility and flagellum
RT defects in Trypanosoma and human.";
RL Nat. Commun. 9:686-686(2018).
CC -!- FUNCTION: Flagellar protein involved in flagellum axoneme organization
CC and function. {ECO:0000269|PubMed:29449551}.
CC -!- SUBCELLULAR LOCATION: Cell projection, cilium, flagellum
CC {ECO:0000269|PubMed:29449551}. Cytoplasm, cytoskeleton, flagellum
CC axoneme {ECO:0000269|PubMed:29449551}. Note=Closely associated to the
CC axoneme along the paraflagellar rod. {ECO:0000269|PubMed:29449551}.
CC -!- SIMILARITY: Belongs to the CFAP44 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AC159429; AAX70050.1; -; Genomic_DNA.
DR EMBL; CP000070; AAZ12420.1; -; Genomic_DNA.
DR RefSeq; XP_845979.1; XM_840886.1.
DR AlphaFoldDB; Q57WH1; -.
DR STRING; 5691.AAZ12420; -.
DR PaxDb; Q57WH1; -.
DR GeneID; 3658565; -.
DR KEGG; tbr:Tb927.7.3560; -.
DR VEuPathDB; TriTrypDB:Tb927.7.3560; -.
DR eggNOG; KOG2106; Eukaryota.
DR InParanoid; Q57WH1; -.
DR OMA; FIMDRVH; -.
DR Proteomes; UP000008524; Chromosome 7.
DR GO; GO:0005930; C:axoneme; IDA:UniProtKB.
DR GO; GO:0031514; C:motile cilium; IEA:UniProtKB-SubCell.
DR GO; GO:0016607; C:nuclear speck; IDA:GeneDB.
DR GO; GO:0035082; P:axoneme assembly; IMP:UniProtKB.
DR GO; GO:0060271; P:cilium assembly; IBA:GO_Central.
DR GO; GO:0060285; P:cilium-dependent cell motility; IMP:UniProtKB.
DR Gene3D; 2.130.10.10; -; 2.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR SMART; SM00320; WD40; 6.
DR SUPFAM; SSF50978; SSF50978; 2.
DR PROSITE; PS50082; WD_REPEATS_2; 1.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 3: Inferred from homology;
KW Cell projection; Cilium; Coiled coil; Cytoplasm; Cytoskeleton; Flagellum;
KW Reference proteome; Repeat; WD repeat.
FT CHAIN 1..1760
FT /note="Cilia- and flagella-associated protein 44"
FT /id="PRO_0000445515"
FT REPEAT 119..160
FT /note="WD 1"
FT /evidence="ECO:0000255"
FT REPEAT 163..202
FT /note="WD 2"
FT /evidence="ECO:0000255"
FT REPEAT 213..251
FT /note="WD 3"
FT /evidence="ECO:0000255"
FT REPEAT 276..315
FT /note="WD 4"
FT /evidence="ECO:0000255"
FT REPEAT 388..427
FT /note="WD 5"
FT /evidence="ECO:0000255"
FT REGION 1155..1224
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1420..1444
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 1348..1389
FT /evidence="ECO:0000255"
FT COMPBIAS 1180..1194
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1209..1224
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1428..1444
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1760 AA; 199208 MW; 027EE84647987FE6 CRC64;
MAAPAQAVVK EKPPIHVYPQ AKIGTKIFTH HGIHSMRYNG VCALTGSTVL MASGRFVMFV
DVHKGTIESM QGPENGGVGA VAVHPSRQYY VVCERKPSDP AIRAYSWPSR TEVGEFVKGA
TKGFSACAFN KDGSMMATVG MYPDFFLTVW DWESRGMVLR SKCHNTDVYT VLFSPFDSGL
LVSGGAGHIK FWTMANTFTG LKLQGLLGKF GRLEISNVSG FVVLSDGKVI SGSESGLIIL
WEGDLIRCCF AREVDREDDD GTAATFMARS YDYTPCHEGA INVVELMEGG RVLMTAGDDG
YFRFWRVSEL EVAEGEGAPP LYVPECLGEI LVHAGAFIRS VTYCKDVDEW VVLDSAGVLW
RVPYVHPDDI LNNAVTKPKE QAVPALEFNG GSITSAALSP IDHTVVTGGE DGTIRLVDYV
TPRELYKMCL PQPNVVIGLR FFQKDPEKKK FLACCKSGAV LLVKRGSTAF TLLGQWRPHN
DGLALFAVDA AEHRLCTIAH GTVFFFTILD DFSSLEPIGF CKIPLPGATC VAWDDASSCC
LIGFECGKLL AIRAPTRDMV DQSVSYEFTC NYALVGIRQR KKVEKKQANV SAGEREGFVE
EEEEEEYLGP WPVRLICPMA DGDFAIGAGG VELLYKYGLH VRYEGQKELP PLPPTGIEPP
DYVEEPLMNL CYRDYTPEAS SMSYSGRYLV VICEGSQMLL RQLDEMGRVR LEPILVASAH
DRLDGPIAAA CTSFDDKMLV SVGSDGLVVA QLLDGCIAPQ PPSPVAQLQP LRAEEIVEPQ
LAPFSITEQK DLDDRRRAED EKRRELNLFL DKLKDVHQKY ARLLRENQSL ALTHRLSKEE
ITIHPQIYRE LQEEMRQRVE ESRKPTALEL ARENIRTRKM RNRFVDNLAH DRFLVRSFSK
EFSVASFRTP YVDGSIKLFQ QQIDELLGSE RCSSLACDGD RGIVSVASGE ASQQSPRNLS
LSTAAGVVRW LNSEERKRNE GEQQKMNAAH REAEILTTTM RQYLNKMDER REERHWRKKG
YEMLLAHKPD PAVEEASLNE ELRRETRRRG ECILRTDPSY HSAPSAVIKL QQLIRLEEII
FNMRNNFSNE LLKLRDEKER LCGTLNVSLQ RIRAINEKLK DKSFHADDVK LTPEEMPGKR
FEISRDGLVA FMKQRQEEKL REQTAKKAQR GFGADLATGE PATNTDTSGA DTPATRRSEG
EDSRKVTISA NRDSFGTAAA RTRSVRTGTL AASKGGRPFS GGGFAAGAAR ERMNHELRVK
HENIKLTEME EEELQIERNR LLAERQRLHT QVQAMMDEFD VRLWSMYEER SRVDANLCLA
HTHSLLLFRE YNILLVFRQK DFELQSSYDE ARNSRDRCLR EMEELQRLVQ DQTASIEKLQ
EANKVFRREV EIFISNSFPA EHVPYITKVF LRQIKRRKHH SDMSGNDDDI TSDDDDDDDM
GEDEAWEEIC PPNCSEERWC EVIEKREVRL DYVDAITEER RQLEATEQRI EEHKALADKN
NAAVSTCLKA IEDFQGEKRK QLNMLETLVA MRCGQVRCLD EEGRCPDTFR RNDLVVVSDK
VITGLHDRIR ALAEEKHDRR GKLKSMVAEQ QALQRERSEK QALHTQWEEK IYEAMLLKFG
QIVNLEVLES SCGSREVEQL KERLRLEELS WEKELRKRDK KIAVLREKLH ESLEYNTSLL
QTIGDQESDR QSVERSLAQS TQKVVSKMYD SINVATEEDR SNLRLLIAAQ QEEIDALRTE
VALLRTKGGH VYAAAMAAGR
