CFA45_PIG
ID CFA45_PIG Reviewed; 549 AA.
AC A0A480NP79; F1RJW3;
DT 23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT 05-JUN-2019, sequence version 1.
DT 03-AUG-2022, entry version 16.
DE RecName: Full=Cilia- and flagella-associated protein 45;
GN Name=CFAP45;
OS Sus scrofa (Pig).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.
OX NCBI_TaxID=9823;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Duroc;
RG Porcine genome sequencing project;
RL Submitted (NOV-2009) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP IDENTIFICATION.
RX PubMed=30723633; DOI=10.7717/peerj.6374;
RA Gilbert D.G.;
RT "Genes of the pig, Sus scrofa, reconstructed with EvidentialGene.";
RL PeerJ 7:E6374-E6374(2019).
RN [3]
RP INTERACTION WITH AK8; CFAP52; DNAH11; DNAI1; DNALI1 AND ENKUR, SUBCELLULAR
RP LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=33139725; DOI=10.1038/s41467-020-19113-0;
RA Dougherty G.W., Mizuno K., Noethe-Menchen T., Ikawa Y., Boldt K.,
RA Ta-Shma A., Aprea I., Minegishi K., Pang Y.P., Pennekamp P., Loges N.T.,
RA Raidt J., Hjeij R., Wallmeier J., Mussaffi H., Perles Z., Elpeleg O.,
RA Rabert F., Shiratori H., Letteboer S.J., Horn N., Young S., Struenker T.,
RA Stumme F., Werner C., Olbrich H., Takaoka K., Ide T., Twan W.K.,
RA Biebach L., Grosse-Onnebrink J., Klinkenbusch J.A., Praveen K.,
RA Bracht D.C., Hoeben I.M., Junger K., Guetzlaff J., Cindric S., Aviram M.,
RA Kaiser T., Memari Y., Dzeja P.P., Dworniczak B., Ueffing M., Roepman R.,
RA Bartscherer K., Katsanis N., Davis E.E., Amirav I., Hamada H., Omran H.;
RT "CFAP45 deficiency causes situs abnormalities and asthenospermia by
RT disrupting an axonemal adenine nucleotide homeostasis module.";
RL Nat. Commun. 11:5520-5520(2020).
CC -!- FUNCTION: Microtubule inner protein (MIP) part of the dynein-decorated
CC doublet microtubules (DMTs) in cilia axoneme, which is required for
CC motile cilia beating (By similarity). It is an AMP-binding protein that
CC may facilitate dynein ATPase-dependent ciliary and flagellar beating
CC via adenine nucleotide homeostasis. May function as a donor of AMP to
CC AK8 and hence promote ADP production (By similarity).
CC {ECO:0000250|UniProtKB:Q32LN4, ECO:0000250|UniProtKB:Q9UL16}.
CC -!- SUBUNIT: Interacts with AK8; dimerization with AK8 may create a cavity
CC at the interface of the dimer that can accommodate AMP
CC (PubMed:33139725). Interacts with CFAP52 (PubMed:33139725). Interacts
CC with ENKUR (PubMed:33139725). Directly interacts with DNALI1
CC (PubMed:33139725). Interacts with DNAH11 (PubMed:33139725). Interacts
CC with DNAI1 (PubMed:33139725). {ECO:0000269|PubMed:33139725}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, cilium axoneme
CC {ECO:0000269|PubMed:33139725}. Cytoplasm, cytoskeleton, flagellum
CC axoneme {ECO:0000269|PubMed:33139725}. Note=Located in the proximal
CC region of respiratory cilia. {ECO:0000250|UniProtKB:Q9UL16}.
CC -!- TISSUE SPECIFICITY: Expressed in respiratory cells (at protein level).
CC {ECO:0000269|PubMed:33139725}.
CC -!- SIMILARITY: Belongs to the CFAP45 family. {ECO:0000305}.
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DR EMBL; AEMK02000022; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; DQIR01143952; HDA99428.1; -; mRNA.
DR EMBL; DQIR01205046; HDB60523.1; -; mRNA.
DR STRING; 9823.ENSSSCP00000006817; -.
DR Ensembl; ENSSSCT00000007007; ENSSSCP00000006817; ENSSSCG00000006394.
DR Ensembl; ENSSSCT00025007006; ENSSSCP00025002875; ENSSSCG00025005205.
DR Ensembl; ENSSSCT00035025353; ENSSSCP00035009586; ENSSSCG00035019569.
DR Ensembl; ENSSSCT00040028893; ENSSSCP00040012121; ENSSSCG00040021455.
DR Ensembl; ENSSSCT00045006728; ENSSSCP00045004598; ENSSSCG00045004043.
DR Ensembl; ENSSSCT00055056656; ENSSSCP00055045297; ENSSSCG00055028529.
DR Ensembl; ENSSSCT00060073635; ENSSSCP00060031753; ENSSSCG00060054085.
DR VGNC; VGNC:86600; CFAP45.
DR eggNOG; ENOG502QPRZ; Eukaryota.
DR GeneTree; ENSGT00730000111174; -.
DR HOGENOM; CLU_026959_1_0_1; -.
DR TreeFam; TF327685; -.
DR Proteomes; UP000008227; Chromosome 4.
DR Proteomes; UP000314985; Unplaced.
DR Bgee; ENSSSCG00000006394; Expressed in testis and 18 other tissues.
DR ExpressionAtlas; A0A480NP79; differential.
DR GO; GO:0097729; C:9+2 motile cilium; IDA:GO_Central.
DR GO; GO:0005879; C:axonemal microtubule; ISS:UniProtKB.
DR GO; GO:0005930; C:axoneme; IDA:GO_Central.
DR InterPro; IPR033253; CFAP45.
DR InterPro; IPR043597; TPH_dom.
DR PANTHER; PTHR15504; PTHR15504; 1.
DR Pfam; PF13868; TPH; 1.
PE 1: Evidence at protein level;
KW Cell projection; Cilium; Coiled coil; Cytoplasm; Cytoskeleton; Flagellum;
KW Reference proteome.
FT CHAIN 1..549
FT /note="Cilia- and flagella-associated protein 45"
FT /id="PRO_0000454203"
FT REGION 1..29
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 388..416
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 119..232
FT /evidence="ECO:0000255"
FT COILED 259..393
FT /evidence="ECO:0000255"
FT COILED 434..522
FT /evidence="ECO:0000255"
FT COMPBIAS 1..20
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 549 AA; 65493 MW; 67A18FAF4304D245 CRC64;
MPLSPAGVLS STSTASNRSR NRPRYRTKAL SSEVDESLFG GIKPLNQSDS PIVLLRDKHA
IRKTLTALGL DHKPETIQLI TRDMVRELII PTKDPSGESL IMSPEEFERI RWASHVPTRE
ELEAKEQACK KEKEAIVDAV TTRKKIMKQK EMVSRNNQKL SDLEEVAKER AQNLLQRASQ
LRMEQEEELK DMKKIILNAK CHAIRDAQIL EKQLIQKELD AEEKRLDQMM EVERQKSIQR
QEELDKKRRE ERIRGRRHIV EQMEKNQEER SLLAEQREQE KEQMLEYMEQ LQEEDLRDLE
RRHQQKLKMQ AEIKRINDEN QKQKAELLAQ EKLADQMVME FTKKKMAREA EFEAEQERIR
REKEKEISRL RALQEKVQDY QAEQDALRAK RNQEVADREW RRKEKENAQK KMETEAQLRK
SRLEQVAFKE HSLAVQVQRD RDEFERILRA QREQIEKEQQ EEEKKAMERL QHANELRRQV
RENQQKQVQD RIAIFEEGQR LKEEAQKRRE RIEGIKKKKI EELRATGLPE KYCIEAERKA
NIPTNTSVN
