CFA46_CHLRE
ID CFA46_CHLRE Reviewed; 2784 AA.
AC A8ICS9; J7FA96;
DT 04-FEB-2015, integrated into UniProtKB/Swiss-Prot.
DT 04-DEC-2007, sequence version 1.
DT 03-AUG-2022, entry version 54.
DE RecName: Full=Cilia- and flagella-associated protein 46 {ECO:0000305};
GN Name=CFAP46 {ECO:0000305}; Synonyms=FAP46 {ECO:0000303|PubMed:22573824};
GN ORFNames=CHLREDRAFT_141109 {ECO:0000312|EMBL:EDP06555.1};
OS Chlamydomonas reinhardtii (Chlamydomonas smithii).
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX NCBI_TaxID=3055;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBUNIT, AND SUBCELLULAR LOCATION.
RX PubMed=22573824; DOI=10.1242/jcs.107151;
RA Brown J.M., DiPetrillo C.G., Smith E.F., Witman G.B.;
RT "A FAP46 mutant provides new insights into the function and assembly of the
RT C1d complex of the ciliary central apparatus.";
RL J. Cell Sci. 125:3904-3913(2012).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CC-503;
RX PubMed=17932292; DOI=10.1126/science.1143609;
RA Merchant S.S., Prochnik S.E., Vallon O., Harris E.H., Karpowicz S.J.,
RA Witman G.B., Terry A., Salamov A., Fritz-Laylin L.K., Marechal-Drouard L.,
RA Marshall W.F., Qu L.H., Nelson D.R., Sanderfoot A.A., Spalding M.H.,
RA Kapitonov V.V., Ren Q., Ferris P., Lindquist E., Shapiro H., Lucas S.M.,
RA Grimwood J., Schmutz J., Cardol P., Cerutti H., Chanfreau G., Chen C.L.,
RA Cognat V., Croft M.T., Dent R., Dutcher S., Fernandez E., Fukuzawa H.,
RA Gonzalez-Ballester D., Gonzalez-Halphen D., Hallmann A., Hanikenne M.,
RA Hippler M., Inwood W., Jabbari K., Kalanon M., Kuras R., Lefebvre P.A.,
RA Lemaire S.D., Lobanov A.V., Lohr M., Manuell A., Meier I., Mets L.,
RA Mittag M., Mittelmeier T., Moroney J.V., Moseley J., Napoli C.,
RA Nedelcu A.M., Niyogi K., Novoselov S.V., Paulsen I.T., Pazour G.J.,
RA Purton S., Ral J.P., Riano-Pachon D.M., Riekhof W., Rymarquis L.,
RA Schroda M., Stern D., Umen J., Willows R., Wilson N., Zimmer S.L.,
RA Allmer J., Balk J., Bisova K., Chen C.J., Elias M., Gendler K., Hauser C.,
RA Lamb M.R., Ledford H., Long J.C., Minagawa J., Page M.D., Pan J.,
RA Pootakham W., Roje S., Rose A., Stahlberg E., Terauchi A.M., Yang P.,
RA Ball S., Bowler C., Dieckmann C.L., Gladyshev V.N., Green P., Jorgensen R.,
RA Mayfield S., Mueller-Roeber B., Rajamani S., Sayre R.T., Brokstein P.,
RA Dubchak I., Goodstein D., Hornick L., Huang Y.W., Jhaveri J., Luo Y.,
RA Martinez D., Ngau W.C., Otillar B., Poliakov A., Porter A., Szajkowski L.,
RA Werner G., Zhou K., Grigoriev I.V., Rokhsar D.S., Grossman A.R.;
RT "The Chlamydomonas genome reveals the evolution of key animal and plant
RT functions.";
RL Science 318:245-250(2007).
RN [3]
RP SUBUNIT, AND SUBCELLULAR LOCATION.
RX PubMed=20421426; DOI=10.1083/jcb.200912009;
RA DiPetrillo C.G., Smith E.F.;
RT "Pcdp1 is a central apparatus protein that binds Ca2+-calmodulin and
RT regulates ciliary motility.";
RL J. Cell Biol. 189:601-612(2010).
CC -!- FUNCTION: As part of the central apparatus of the cilium axoneme plays
CC a role in cilium movement and thereby cell motility.
CC {ECO:0000269|PubMed:22573824}.
CC -!- SUBUNIT: Part of the PDCP1 complex composed of CFAP46, CFAP54, CFAP74
CC and CFAP221; the PDCP1 complex binds calmodulin.
CC {ECO:0000269|PubMed:20421426, ECO:0000269|PubMed:22573824}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, cilium axoneme
CC {ECO:0000269|PubMed:20421426, ECO:0000269|PubMed:22573824}.
CC -!- SIMILARITY: Belongs to the CFAP46 family. {ECO:0000305}.
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DR EMBL; JF827824; AFG30956.1; -; mRNA.
DR EMBL; DS496115; EDP06555.1; -; Genomic_DNA.
DR RefSeq; XP_001702776.1; XM_001702724.1.
DR PDB; 7SQC; EM; 3.80 A; R0/R1=1-2784.
DR PDBsum; 7SQC; -.
DR STRING; 3055.EDP06555; -.
DR PaxDb; A8ICS9; -.
DR EnsemblPlants; PNW77067; PNW77067; CHLRE_10g420800v5.
DR GeneID; 5728279; -.
DR Gramene; PNW77067; PNW77067; CHLRE_10g420800v5.
DR KEGG; cre:CHLRE_10g420800v5; -.
DR eggNOG; ENOG502QS2Z; Eukaryota.
DR HOGENOM; CLU_226927_0_0_1; -.
DR InParanoid; A8ICS9; -.
DR OMA; EKNYGQA; -.
DR OrthoDB; 117168at2759; -.
DR GO; GO:0005930; C:axoneme; IDA:UniProtKB.
DR GO; GO:0035082; P:axoneme assembly; IMP:UniProtKB.
DR GO; GO:0060294; P:cilium movement involved in cell motility; IMP:UniProtKB.
DR InterPro; IPR039586; CFAP46.
DR PANTHER; PTHR15977; PTHR15977; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell projection; Cilium; Cilium biogenesis/degradation;
KW Coiled coil; Cytoplasm; Cytoskeleton; Repeat; TPR repeat.
FT CHAIN 1..2784
FT /note="Cilia- and flagella-associated protein 46"
FT /id="PRO_0000431701"
FT REPEAT 129..162
FT /note="TPR 1"
FT /evidence="ECO:0000255"
FT REPEAT 401..434
FT /note="TPR 2"
FT /evidence="ECO:0000255"
FT REPEAT 708..743
FT /note="TPR 3"
FT /evidence="ECO:0000255"
FT REPEAT 976..1009
FT /note="TPR 4"
FT /evidence="ECO:0000255"
FT REPEAT 1712..1745
FT /note="TPR 5"
FT /evidence="ECO:0000255"
FT REPEAT 1854..1886
FT /note="TPR 6"
FT /evidence="ECO:0000255"
FT REPEAT 2613..2646
FT /note="TPR 7"
FT /evidence="ECO:0000255"
FT REGION 543..562
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 581..607
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 670..697
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 799..837
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 929..954
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1275..1351
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1640..1673
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2068..2112
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2278..2303
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2346..2389
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2441..2465
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 242..268
FT /evidence="ECO:0000255"
FT COILED 644..665
FT /evidence="ECO:0000255"
FT COILED 1961..1984
FT /evidence="ECO:0000255"
FT COMPBIAS 803..817
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2068..2083
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2353..2367
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2449..2465
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 57
FT /note="K -> E (in Ref. 1; AFG30956)"
FT /evidence="ECO:0000305"
FT CONFLICT 172
FT /note="D -> E (in Ref. 1; AFG30956)"
FT /evidence="ECO:0000305"
FT CONFLICT 210
FT /note="A -> V (in Ref. 1; AFG30956)"
FT /evidence="ECO:0000305"
FT CONFLICT 361
FT /note="E -> D (in Ref. 1; AFG30956)"
FT /evidence="ECO:0000305"
FT CONFLICT 524
FT /note="A -> T (in Ref. 1; AFG30956)"
FT /evidence="ECO:0000305"
FT CONFLICT 551
FT /note="E -> K (in Ref. 1; AFG30956)"
FT /evidence="ECO:0000305"
FT CONFLICT 642
FT /note="D -> A (in Ref. 1; AFG30956)"
FT /evidence="ECO:0000305"
FT CONFLICT 671
FT /note="R -> Q (in Ref. 1; AFG30956)"
FT /evidence="ECO:0000305"
FT CONFLICT 725
FT /note="T -> A (in Ref. 1; AFG30956)"
FT /evidence="ECO:0000305"
FT CONFLICT 925
FT /note="S -> L (in Ref. 1; AFG30956)"
FT /evidence="ECO:0000305"
FT CONFLICT 1056
FT /note="G -> D (in Ref. 1; AFG30956)"
FT /evidence="ECO:0000305"
FT CONFLICT 1135
FT /note="R -> W (in Ref. 1; AFG30956)"
FT /evidence="ECO:0000305"
FT CONFLICT 1155
FT /note="A -> S (in Ref. 1; AFG30956)"
FT /evidence="ECO:0000305"
FT CONFLICT 1165
FT /note="K -> N (in Ref. 1; AFG30956)"
FT /evidence="ECO:0000305"
FT CONFLICT 1414
FT /note="G -> A (in Ref. 1; AFG30956)"
FT /evidence="ECO:0000305"
FT CONFLICT 1438
FT /note="V -> A (in Ref. 1; AFG30956)"
FT /evidence="ECO:0000305"
FT CONFLICT 1830
FT /note="A -> D (in Ref. 1; AFG30956)"
FT /evidence="ECO:0000305"
FT CONFLICT 1906
FT /note="E -> D (in Ref. 1; AFG30956)"
FT /evidence="ECO:0000305"
FT CONFLICT 1957
FT /note="C -> R (in Ref. 1; AFG30956)"
FT /evidence="ECO:0000305"
FT CONFLICT 2053
FT /note="A -> S (in Ref. 1; AFG30956)"
FT /evidence="ECO:0000305"
FT CONFLICT 2093
FT /note="E -> EED (in Ref. 1; AFG30956)"
FT /evidence="ECO:0000305"
FT CONFLICT 2344..2348
FT /note="SAAAK -> PAAAR (in Ref. 1; AFG30956)"
FT /evidence="ECO:0000305"
FT CONFLICT 2375
FT /note="R -> G (in Ref. 1; AFG30956)"
FT /evidence="ECO:0000305"
FT CONFLICT 2405
FT /note="V -> A (in Ref. 1; AFG30956)"
FT /evidence="ECO:0000305"
FT CONFLICT 2447
FT /note="G -> S (in Ref. 1; AFG30956)"
FT /evidence="ECO:0000305"
FT CONFLICT 2491
FT /note="T -> N (in Ref. 1; AFG30956)"
FT /evidence="ECO:0000305"
FT CONFLICT 2515..2518
FT /note="SAAA -> AAAT (in Ref. 1; AFG30956)"
FT /evidence="ECO:0000305"
FT CONFLICT 2659
FT /note="D -> E (in Ref. 1; AFG30956)"
FT /evidence="ECO:0000305"
FT CONFLICT 2693
FT /note="V -> A (in Ref. 1; AFG30956)"
FT /evidence="ECO:0000305"
FT CONFLICT 2724
FT /note="V -> A (in Ref. 1; AFG30956)"
FT /evidence="ECO:0000305"
FT CONFLICT 2743
FT /note="G -> S (in Ref. 1; AFG30956)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 2784 AA; 289251 MW; 22CD7592A4396E0B CRC64;
MAQPETLHQV LAQPADASRV KQLQTVHEAI TASLSGQQPG ARVGLETLVL CAEAALKVGS
LDVAHDCLER YFLEQRRYTV GMAPVELRDQ YLCRAHFARG LLVSERSKGL KGKALIDGTL
EGVKHVMAGL EVAAANQPRY QFLVYNASVH HWRVVAPLHR DGLRHHLLAS TDRVVQALDK
VPGHEEWKVR IYTALALCQS DAATVAAAQA EAAGGKAPAK GGGGAAATHD DAAKTLQRAY
DTAAAAKLTE LQKDVARLQV HLATLAAAAG GKGAKAGGGG AAGGAGKPGA KGAAAALDEE
GALRLIQAVL TGRPDRAVAE QQLREAVAKV DPKGPDGPVK GALLRPVSRA AWAAALAGLP
ELAERWASRA AASSDSGPRT WSDMTRVQLA LQALGPAGDT SLAPVVVAAH VKALEQLEDV
LTTFTKLADV EGIHAAARLA WNAGLLLLQP GLRKHVKRAF NAAARALAVA ASPLTRLRAA
LHLEAGKCDA AEEALIKAGQ EVGKALALDY LPPPAEAAAV PWLARPLDRW AAPLARALAL
RTSAEPANPE EEAVSLIERS KESRNPAIKS DLLARAREKL RDLPHPPPPA PTDPPGPDRD
AAHAAARRRT AVWAELMRSA ASSRMDEHVL AAAPHALCVS WDPAVDREMV LLQAQLAHYE
AEAAISALRR RRADISPPTR PSPPEVDGEG VRQPPATTEQ LQELVVQASV RSMRGAMSVN
EPWLTLNNAV QLYNAALPLM QQHRYADLYR WLRPVAEALV ALPVADSDGP LAVAVAEALG
RAAEHRLLLA TLRAKAARDA GQELEDDDDE DSLDEDGNPP PAGDAGPHFN RRSPAYKPLP
DARVAASGLD PRGFPALARL LASIGTVTAV AEAALERAGG ASTQGLLEMY ARLQQYRGVS
SGLPAGAAAA SAATSRVVSA IEALSSANRV NEEKQPAGAG AEKGGGDKGR KPHGSLAADA
AAAIALLRAL PGGPPLELWA KMARAVADAG VWPAALECSA AALAALPGAG RDLDVLRLEA
PSDVPEMTPA GWFWASVALS VRAAALLTLV DLPSQGAATA LTVRREALLH AAQAARCAAF
VNKADLCESA CRVAWNAALP FTTKPLLRAA LIRPLGTAVE ALNRVGPADK GFQVRMNCLY
VESLAAAKRW GDAVAACDAA SRAVKSRSLH RPLMGWKAAC LAMLGKNVNA EMTKVKEHPP
EAQAYAWSVL SHHSAARYDQ IAAHKAAGEA VEHHGWLKAV ALAGYAEWLL SSSDDKEAAE
DALLAAADAL LEFDTGDLDG DGTDDEDDAT KAKPRSRSGG GSSSGRAGGG FRRVLSRSYS
RGGGGSADGA RPSEGGEGAG PAAPDPNRVP EELGSTHLER LARLYVMACQ AAPNATDHTD
YMLAAHHNFM RLLTQALHSA AHTAFVAARD SYNGEVAAAA LEGRDPPPET PLPKPYAVVP
DTLIGWGTWQ ITPELLETLA SDAATGAVTA LSRELLPQAE LTLAYLELLQ ACLRARGYHC
HCLGLAQLQR VLARMVLRDE GMYVATSLGL VAALDELGLS REAGEVEAAL GDVACIGPQE
EAQAAEQAAL ADLVLAAAGK LEAARPRSAL AVGRLSFLAG GVRRANNAAN AAAAAPLPPV
AAASQAAAAA DAAAAASFTA AGGGRGGRES PSPHDDGIHY IGGPAPGDSH GQLPEWMEDA
ARELGNVDLA GSAGGLLSHV SGQLLLRPFT LHDVWLKKGD YLLRRGHYAA ARQLLSRARA
HAADCGNREA EARCLLALSR TELAAHNPVE AVALVQAAQR FGGDIDFWAE LLVQYVDCRL
AGAHSTTSDA REALQGGIAM FLALARDDRA AEKPASAAGA VLRVRLARLL LTDMEMLRGQ
GVSTWRKSYD GAVTLVNQAI MALAAREAGL PHIEALLVQA ELMLAEPTHI KDLRPRLKKV
EKVLLAAEEM AVRFHAEATP RDLAPRCVTP TARLLACVRC RLAEVQLAAA EERERLAGAD
REKARPKFPH MRGKRDVQVV IDFIDEAGGA PPAPPGLLPE DSALALATGA AALVAAAPRD
RARALLIAGR CLAFKFMMAA PEALDPLRPF VPPPKPPGAP KRPPAGAEEE EDEEGPDTAA
ADAAAEAAEA AATPEGAAAL RLQAQAAAML SSALASATSV QDWALGEQCA LALSTLYGQL
SPGLACRSLA AAQACRAAAG GQALLRAAAP AQQPEVLALA QRDKLSEVLP APQDNVHYSA
LRRMLSGLKG AAARLDAAAA PPVEQQLAAL PQDLRVLMLY LSPDGGRLYA AALNIPDATA
EPTPPLNAEK SKKKTDASAP AAAGPRLSLL HVVEVDPAAV EALVAECRAY RRGVERTLRE
AIASAAAKGV GVPPEDDRPD SPSKKGKKPG SATKRPGSKQ GPKSGPGAAA AAAAAAAAAG
EAGKVQVFDS RLNEEWSGIL EQFEEWLAPL APWLEAAVPA LPLPPPGSPD GKKEKKDKKE
AAGPTKHKVA LLLDPALQSL PWEAARHLAT TCSEVSRSPS LQALAACHTL PRADSAAASE
HAAAAAAAPA ALPPLDLGRL TVIVDPRHEC STAQQARGPY TAQLIPALSA PELTQVLPAA
SWWGPGGPGG GLEGVPGRAP SPDTYASLLA GQATGGPCTG LLFLGVGRFA AHVPPAVLAS
APLGGCEAAL LFDRCNTDDA YWAQLYRDNR KSAEQRRLES PGRVATLLLA KGVRTVLVMS
AAAPPAAVVK LMHGVMAGLA AGRVLGEVVY SLLTGGGGSG GAGAGVLDEF ELAHLRACLQ
VWGAPGLLGA VLTQGNKAAK GAKK
