CFA52_MOUSE
ID CFA52_MOUSE Reviewed; 620 AA.
AC Q5F201; Q5F200; Q9D432; Q9DA68;
DT 02-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 15-MAR-2005, sequence version 1.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Cilia- and flagella-associated protein 52 {ECO:0000312|MGI:MGI:1919110};
DE AltName: Full=WD repeat-containing protein 16 {ECO:0000312|MGI:MGI:1919110};
GN Name=Cfap52 {ECO:0000312|MGI:MGI:1919110};
GN Synonyms=Wdr16 {ECO:0000312|MGI:MGI:1919110};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Microtubule inner protein (MIP) part of the dynein-decorated
CC doublet microtubules (DMTs) in cilia axoneme, which is required for
CC motile cilia beating (By similarity). Important for proper ciliary and
CC flagellar beating. May act in cooperation with CFAP45 and axonemal
CC dynein subunit DNAH11. May play a role in cell growth and/or survival
CC (By similarity). {ECO:0000250|UniProtKB:E1BKF9,
CC ECO:0000250|UniProtKB:Q8N1V2}.
CC -!- SUBUNIT: Interacts with BRCA2 (By similarity). Interacts with the CCT
CC chaperonin complex (By similarity). Interacts with HSP70 (By
CC similarity). Interacts with AK8 (By similarity). Interacts with CFAP45
CC (By similarity). Interacts with DNAI1 (By similarity). Interacts with
CC IQDC (By similarity). {ECO:0000250|UniProtKB:F1SS88,
CC ECO:0000250|UniProtKB:Q8N1V2}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q8N1V2}. Cell
CC projection, cilium, flagellum {ECO:0000250|UniProtKB:A8ILK1}.
CC Cytoplasm, cytoskeleton, cilium axoneme {ECO:0000250|UniProtKB:Q8N1V2}.
CC Note=Located in the proximal region of respiratory cilia.
CC {ECO:0000250|UniProtKB:Q8N1V2}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q5F201-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q5F201-2; Sequence=VSP_018069, VSP_018070;
CC -!- SIMILARITY: Belongs to the CFAP52 family. {ECO:0000305}.
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DR EMBL; AK006118; BAB24416.1; -; mRNA.
DR EMBL; AK016839; BAB30457.1; -; mRNA.
DR EMBL; AL663076; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL732570; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS24862.1; -. [Q5F201-1]
DR RefSeq; NP_082239.2; NM_027963.2. [Q5F201-1]
DR AlphaFoldDB; Q5F201; -.
DR SMR; Q5F201; -.
DR STRING; 10090.ENSMUSP00000021287; -.
DR iPTMnet; Q5F201; -.
DR PhosphoSitePlus; Q5F201; -.
DR MaxQB; Q5F201; -.
DR PaxDb; Q5F201; -.
DR PRIDE; Q5F201; -.
DR ProteomicsDB; 281538; -. [Q5F201-1]
DR ProteomicsDB; 281539; -. [Q5F201-2]
DR Antibodypedia; 12661; 102 antibodies from 19 providers.
DR Ensembl; ENSMUST00000021287; ENSMUSP00000021287; ENSMUSG00000020904. [Q5F201-1]
DR Ensembl; ENSMUST00000126766; ENSMUSP00000116496; ENSMUSG00000020904. [Q5F201-2]
DR GeneID; 71860; -.
DR KEGG; mmu:71860; -.
DR UCSC; uc007jnf.1; mouse. [Q5F201-1]
DR UCSC; uc007jng.1; mouse. [Q5F201-2]
DR CTD; 146845; -.
DR MGI; MGI:1919110; Cfap52.
DR VEuPathDB; HostDB:ENSMUSG00000020904; -.
DR eggNOG; KOG0266; Eukaryota.
DR GeneTree; ENSGT00940000157016; -.
DR HOGENOM; CLU_009244_2_0_1; -.
DR InParanoid; Q5F201; -.
DR OMA; LLRIMVY; -.
DR PhylomeDB; Q5F201; -.
DR TreeFam; TF323254; -.
DR BioGRID-ORCS; 71860; 3 hits in 72 CRISPR screens.
DR PRO; PR:Q5F201; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q5F201; protein.
DR Bgee; ENSMUSG00000020904; Expressed in otolith organ and 96 other tissues.
DR ExpressionAtlas; Q5F201; baseline and differential.
DR Genevisible; Q5F201; MM.
DR GO; GO:0097729; C:9+2 motile cilium; ISO:MGI.
DR GO; GO:0005879; C:axonemal microtubule; ISS:UniProtKB.
DR GO; GO:0005930; C:axoneme; ISO:MGI.
DR GO; GO:0060271; P:cilium assembly; IBA:GO_Central.
DR GO; GO:0061966; P:establishment of left/right asymmetry; ISO:MGI.
DR GO; GO:0030317; P:flagellated sperm motility; ISO:MGI.
DR Gene3D; 2.130.10.10; -; 3.
DR InterPro; IPR033015; CFAP52.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR13720:SF14; PTHR13720:SF14; 1.
DR Pfam; PF00400; WD40; 5.
DR SMART; SM00320; WD40; 11.
DR SUPFAM; SSF50978; SSF50978; 2.
DR PROSITE; PS00678; WD_REPEATS_1; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 5.
DR PROSITE; PS50294; WD_REPEATS_REGION; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell projection; Cilium; Cytoplasm; Cytoskeleton;
KW Flagellum; Reference proteome; Repeat; WD repeat.
FT CHAIN 1..620
FT /note="Cilia- and flagella-associated protein 52"
FT /evidence="ECO:0000305"
FT /id="PRO_0000233155"
FT REPEAT 62..106
FT /note="WD 1"
FT REPEAT 109..150
FT /note="WD 2"
FT REPEAT 156..195
FT /note="WD 3"
FT REPEAT 288..327
FT /note="WD 4"
FT REPEAT 330..369
FT /note="WD 5"
FT REPEAT 372..411
FT /note="WD 6"
FT REPEAT 415..454
FT /note="WD 7"
FT REPEAT 459..498
FT /note="WD 8"
FT REPEAT 500..541
FT /note="WD 9"
FT REPEAT 543..582
FT /note="WD 10"
FT REPEAT 585..620
FT /note="WD 11"
FT VAR_SEQ 342
FT /note="F -> L (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_018069"
FT VAR_SEQ 343..620
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_018070"
FT CONFLICT 309
FT /note="T -> A (in Ref. 1; BAB30457)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 620 AA; 68240 MW; 85062659B988E2AC CRC64;
MEEQVLPELD VAELELQAVI GFNGHVPNGL KCHPDQEHLI YPLGCTVLIQ AINTNEQNFL
HGHGNNVSCV TISKEGDYIA SGQVTFMGFK ADIILWDFKK RELIARLSLH KGKIEALAFS
PNDLYLVSLG GPDDGSVVVW SIAKRDAICG SPAAGLNVGN ATSVVFSRCR DEMFVTAGNG
TIRVWELDLP NRKIWPTECQ TGQMKRIVLS TGMADDDSFF YLGTTTGDIL KMNPKTKLLA
DTGPVKDKFS LGVSALRCLK MGGLLVGSGA GLLIFCKSPS YKPIKKVQLQ GGITSITLRG
EGHQFFVGTE ESHIYRVNFT DFKETLIATC HFEAVQDIVF PFGTAELFAT CAKKDIRVWH
TMSKRELLRI TVPNMTCHGI DFMRDGKSII SAWDDGKIRA FAPESGRLMY TINSAHRIGV
TAIATTSDCK RIISGGGEGE VRVWQVGCQT QKLEEALKEH KSSVSCIRVK KNNEECVTAS
TDGTCIIWDL VRLRRNQMIL ANTLFQCVCY HPEEFQIITS GTDRKIAYWE VFDGSVIREL
EGSLSGSING MDITQEGGHF VTGGHDHLVK VWDYNEGEVT HVGVGHSGNI MAMRISPGNQ
YIVSVSADGA ILRWKYPFAS
