ID   CFA53_BOVIN             Reviewed;         514 AA.
AC   F1N7G5;
DT   25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT   10-APR-2019, sequence version 3.
DT   03-AUG-2022, entry version 54.
DE   RecName: Full=Cilia- and flagella-associated protein 53;
GN   Name=CFAP53; Synonyms=MBD1 {ECO:0000312|VGNC:VGNC:31273};
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913 {ECO:0000312|Proteomes:UP000009136};
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Hereford {ECO:0000312|Proteomes:UP000009136};
RA   Rosen B.D., Bickhart D.M., Koren S., Schnabel R.D., Hall R., Zimin A.,
RA   Dreischer C., Schultheiss S., Schroeder S.G., Elsik C.G., Couldrey C.,
RA   Liu G.E., Van Tassell C.P., Phillippy A.M., Smith T.P.L., Medrano J.F.;
RT   "ARS-UCD1.2.";
RL   Submitted (MAR-2018) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0007744|PDB:7RRO}
RP   STRUCTURE BY ELECTRON MICROSCOPY (3.40 ANGSTROMS), INTERACTION WITH PIERCE1
RP   AND PIERCE2, SUBCELLULAR LOCATION, FUNCTION, AND TISSUE SPECIFICITY.
RX   PubMed=34715025; DOI=10.1016/j.cell.2021.10.007;
RA   Gui M., Farley H., Anujan P., Anderson J.R., Maxwell D.W., Whitchurch J.B.,
RA   Botsch J.J., Qiu T., Meleppattu S., Singh S.K., Zhang Q., Thompson J.,
RA   Lucas J.S., Bingle C.D., Norris D.P., Roy S., Brown A.;
RT   "De novo identification of mammalian ciliary motility proteins using cryo-
RT   EM.";
RL   Cell 184:5791-5806.e19(2021).
CC   -!- FUNCTION: Microtubule inner protein (MIP) part of the dynein-decorated
CC       doublet microtubules (DMTs) in cilia axoneme, which is required for
CC       motile cilia beating (PubMed:34715025). May play a role in the beating
CC       of primary cilia and thereby be involved in the establishment of organ
CC       laterality during embryogenesis (PubMed:34715025).
CC       {ECO:0000269|PubMed:34715025}.
CC   -!- SUBUNIT: Interacts with PIERCE1 and PIERCE2; the interactions link
CC       outer dynein arms docking complex (ODA-DC) to the internal microtubule
CC       inner proteins (MIP) in cilium axoneme. {ECO:0000269|PubMed:34715025}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, cilium axoneme
CC       {ECO:0000269|PubMed:34715025}.
CC   -!- TISSUE SPECIFICITY: Expressed in trachea multiciliated cells.
CC       {ECO:0000269|PubMed:34715025}.
CC   -!- SIMILARITY: Belongs to the CFAP53 family. {ECO:0000305}.
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DR   RefSeq; NP_001291505.1; NM_001304576.1.
DR   PDB; 7RRO; EM; 3.40 A; 3/4=1-514.
DR   PDBsum; 7RRO; -.
DR   STRING; 9913.ENSBTAP00000003444; -.
DR   PaxDb; F1N7G5; -.
DR   Ensembl; ENSBTAT00000003444; ENSBTAP00000003444; ENSBTAG00000003801.
DR   GeneID; 540193; -.
DR   KEGG; bta:540193; -.
DR   CTD; 220136; -.
DR   VEuPathDB; HostDB:ENSBTAG00000003801; -.
DR   VGNC; VGNC:31273; MBD1.
DR   eggNOG; ENOG502QRDR; Eukaryota.
DR   GeneTree; ENSGT00950000183005; -.
DR   HOGENOM; CLU_036489_1_0_1; -.
DR   InParanoid; F1N7G5; -.
DR   OMA; MQIAHQQ; -.
DR   OrthoDB; 1167111at2759; -.
DR   TreeFam; TF329393; -.
DR   Proteomes; UP000009136; Chromosome 24.
DR   Bgee; ENSBTAG00000003801; Expressed in spermatid and 108 other tissues.
DR   ExpressionAtlas; F1N7G5; baseline and differential.
DR   GO; GO:0005879; C:axonemal microtubule; IDA:UniProtKB.
DR   GO; GO:0060271; P:cilium assembly; IEA:InterPro.
DR   GO; GO:0003341; P:cilium movement; IEA:InterPro.
DR   InterPro; IPR033365; CFAP53.
DR   InterPro; IPR043596; CFAP53/TCHP.
DR   InterPro; IPR043597; TPH_dom.
DR   PANTHER; PTHR31183; PTHR31183; 1.
DR   PANTHER; PTHR31183:SF1; PTHR31183:SF1; 1.
DR   Pfam; PF13868; TPH; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cell projection; Cilium; Coiled coil; Cytoplasm;
KW   Cytoskeleton; Developmental protein; Reference proteome.
FT   CHAIN           1..514
FT                   /note="Cilia- and flagella-associated protein 53"
FT                   /id="PRO_0000455519"
FT   COILED          207..429
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   514 AA;  62015 MW;  11D47A09B75E0E1F CRC64;
     MYSQRFGIVQ REVKGPTPKV VIVRAKPPKG QGAEHHLQRI QHSHQKHHAI LASIKSIERD
     RLKTEWDQHN DCKFVDSLVK ARVKDAMQGF IINTEERRNK LRELLASEEN EYFTEMQLKE
     ETIEEKKDRM RDKIRLLREK KEKERQDFVA EKLDQQFRER CQELRAELFC IHQKAVCEER
     KAQIAFNEEL KRQKVVEEQM FSKLWEEDRL AKERREAKEE RRQKELVENT RLGLNAQVTS
     IQAQRQAAQR LKEEEALLVE NENAQVKLEN EQDKLKKQKT KQEIRAALQK ALQEKMERMQ
     QEYREEQDLN MKLMQNALQS LQEETDKKKQ KKEDMRREQK IYYQYLAQRH EEEKAQEKEL
     DRMLEKEKEK KFAEKDKELR LEKEARKQLL NEVMCTRKLQ VQEKLQRKAK EQEERTMEQE
     RINEGLKELN CEERENFIRR CSLAQEYRKQ LQMQICSQQQ AREAEEEEER REFEAGIAAE
     KSFQDKIQGI LSTHQVVPRN IHPMRRACST KLPP