CFA54_CHLRE
ID CFA54_CHLRE Reviewed; 3081 AA.
AC A8J666; J7F8V0;
DT 04-FEB-2015, integrated into UniProtKB/Swiss-Prot.
DT 04-DEC-2007, sequence version 1.
DT 03-AUG-2022, entry version 62.
DE RecName: Full=Cilia- and flagella-associated protein 54 {ECO:0000305};
DE AltName: Full=Flagella-associated protein 54 {ECO:0000303|PubMed:22573824};
GN Name=CFAP54 {ECO:0000305}; Synonyms=FAP54 {ECO:0000303|PubMed:22573824};
GN ORFNames=CHLREDRAFT_150841 {ECO:0000312|EMBL:EDP00642.1};
OS Chlamydomonas reinhardtii (Chlamydomonas smithii).
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX NCBI_TaxID=3055;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RX PubMed=22573824; DOI=10.1242/jcs.107151;
RA Brown J.M., DiPetrillo C.G., Smith E.F., Witman G.B.;
RT "A FAP46 mutant provides new insights into the function and assembly of the
RT C1d complex of the ciliary central apparatus.";
RL J. Cell Sci. 125:3904-3913(2012).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CC-503;
RX PubMed=17932292; DOI=10.1126/science.1143609;
RA Merchant S.S., Prochnik S.E., Vallon O., Harris E.H., Karpowicz S.J.,
RA Witman G.B., Terry A., Salamov A., Fritz-Laylin L.K., Marechal-Drouard L.,
RA Marshall W.F., Qu L.H., Nelson D.R., Sanderfoot A.A., Spalding M.H.,
RA Kapitonov V.V., Ren Q., Ferris P., Lindquist E., Shapiro H., Lucas S.M.,
RA Grimwood J., Schmutz J., Cardol P., Cerutti H., Chanfreau G., Chen C.L.,
RA Cognat V., Croft M.T., Dent R., Dutcher S., Fernandez E., Fukuzawa H.,
RA Gonzalez-Ballester D., Gonzalez-Halphen D., Hallmann A., Hanikenne M.,
RA Hippler M., Inwood W., Jabbari K., Kalanon M., Kuras R., Lefebvre P.A.,
RA Lemaire S.D., Lobanov A.V., Lohr M., Manuell A., Meier I., Mets L.,
RA Mittag M., Mittelmeier T., Moroney J.V., Moseley J., Napoli C.,
RA Nedelcu A.M., Niyogi K., Novoselov S.V., Paulsen I.T., Pazour G.J.,
RA Purton S., Ral J.P., Riano-Pachon D.M., Riekhof W., Rymarquis L.,
RA Schroda M., Stern D., Umen J., Willows R., Wilson N., Zimmer S.L.,
RA Allmer J., Balk J., Bisova K., Chen C.J., Elias M., Gendler K., Hauser C.,
RA Lamb M.R., Ledford H., Long J.C., Minagawa J., Page M.D., Pan J.,
RA Pootakham W., Roje S., Rose A., Stahlberg E., Terauchi A.M., Yang P.,
RA Ball S., Bowler C., Dieckmann C.L., Gladyshev V.N., Green P., Jorgensen R.,
RA Mayfield S., Mueller-Roeber B., Rajamani S., Sayre R.T., Brokstein P.,
RA Dubchak I., Goodstein D., Hornick L., Huang Y.W., Jhaveri J., Luo Y.,
RA Martinez D., Ngau W.C., Otillar B., Poliakov A., Porter A., Szajkowski L.,
RA Werner G., Zhou K., Grigoriev I.V., Rokhsar D.S., Grossman A.R.;
RT "The Chlamydomonas genome reveals the evolution of key animal and plant
RT functions.";
RL Science 318:245-250(2007).
RN [3]
RP SUBUNIT, AND SUBCELLULAR LOCATION.
RX PubMed=20421426; DOI=10.1083/jcb.200912009;
RA DiPetrillo C.G., Smith E.F.;
RT "Pcdp1 is a central apparatus protein that binds Ca2+-calmodulin and
RT regulates ciliary motility.";
RL J. Cell Biol. 189:601-612(2010).
CC -!- SUBUNIT: Part of the PDCP1 complex composed of CFAP46, CFAP54, CFAP74
CC and CFAP221; the PDCP1 complex binds calmodulin.
CC {ECO:0000269|PubMed:20421426}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, cilium axoneme
CC {ECO:0000269|PubMed:20421426}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=A8J666-1; Sequence=Displayed;
CC Name=2;
CC IsoId=A8J666-2; Sequence=VSP_057368;
CC -!- MISCELLANEOUS: [Isoform 1]: Gene prediction.
CC -!- SIMILARITY: Belongs to the CFAP54 family. {ECO:0000305}.
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DR EMBL; JF827825; AFG30957.1; -; mRNA.
DR EMBL; DS496138; EDP00642.1; -; Genomic_DNA.
DR RefSeq; XP_001696950.1; XM_001696898.1. [A8J666-1]
DR PDB; 7SQC; EM; 3.80 A; Q0/Q1=1-3081.
DR PDBsum; 7SQC; -.
DR STRING; 3055.EDP00642; -.
DR PaxDb; A8J666; -.
DR EnsemblPlants; PNW75238; PNW75238; CHLRE_12g518550v5. [A8J666-2]
DR GeneID; 5722626; -.
DR Gramene; PNW75238; PNW75238; CHLRE_12g518550v5. [A8J666-2]
DR eggNOG; ENOG502QVDY; Eukaryota.
DR HOGENOM; CLU_225847_0_0_1; -.
DR InParanoid; A8J666; -.
DR OMA; TCHELVE; -.
DR OrthoDB; 87192at2759; -.
DR GO; GO:0005930; C:axoneme; IDA:UniProtKB.
DR InterPro; IPR027912; CFAP54.
DR Pfam; PF14858; CFAP54_N; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Cell projection; Cilium; Cytoplasm;
KW Cytoskeleton.
FT CHAIN 1..3081
FT /note="Cilia- and flagella-associated protein 54"
FT /id="PRO_0000431704"
FT REGION 542..579
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 591..626
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 910..942
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1406..1451
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1518..1586
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1636..1657
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2176..2210
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2229..2306
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2776..2806
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 564..579
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1426..1449
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1533..1552
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2288..2303
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 984
FT /note="I -> IQGLRTNDTYLFAVALYDDDGNIVGGLGTSTPEVLVALPLPLLMCWT
FT HLLAAAVRCGAAGAAAAKRAASVLLPHFVVTTPDVPLWRSNPMDAQRLHRAHVAAAARP
FT LLRALVQAIYLYGGAALLRNGGGGGGGGGGSTAPGAPVP (in isoform 2)"
FT /id="VSP_057368"
FT CONFLICT 15
FT /note="E -> G (in Ref. 1; AFG30957)"
FT /evidence="ECO:0000305"
FT CONFLICT 62
FT /note="A -> E (in Ref. 1; AFG30957)"
FT /evidence="ECO:0000305"
FT CONFLICT 336
FT /note="V -> A (in Ref. 1; AFG30957)"
FT /evidence="ECO:0000305"
FT CONFLICT 2286
FT /note="A -> V (in Ref. 1; AFG30957)"
FT /evidence="ECO:0000305"
FT CONFLICT 2313
FT /note="L -> P (in Ref. 1; AFG30957)"
FT /evidence="ECO:0000305"
FT CONFLICT 2337
FT /note="A -> V (in Ref. 1; AFG30957)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 3081 AA; 317779 MW; F1A360BBF703E075 CRC64;
MASEVIALCH SFEQELAKSL NVLPPVSASK PDAHDAHLNH HRLSQRIAES VSYYAGRLPA
YASVPRILVF GDKLFRAEQY QLALQACYKH IRGLELHSSR ENLPRMDAQA RLSSHVQACF
GCAACEAALL LASDGSVKHP DTLQWLVSCL AQLRAAMSLA LPDERLYWLV LNGTVHVYGI
AKAMITAGFA EQALPALVFC IKALEGHVAF AAPKYLPWRT QLYTWAVYGL ADCGAVEQAR
ALLADGLKRL EMLVALQKLD PVPAAPAVQA AFAAARGALV GLQLRVEMAA GAAVTPVLAQ
LSAGAAGAGA VGAAGPTARA GLAALVEALH VPHRRVVRTE AVSGGPLKEL FDAAMAVAAP
LIGDLKKATE AAAAADAAAA AAAVAADAAS AEAETAGGAD AAAPADTGAQ QQASAAAAAA
DEAAVAASAA LGAAQEALPC ALHKGLLCAA YNLEQWKQFE ELASLATSRS DVVYADAVPT
GTGSTTTTAA AAADVDQTAA QLGTAASILT ALRQLSTAPG VESLRTLAAM LQQALSRGIT
VSGTAGAAPP PPPRRVSMTG MLAGGSASPP NGSSGAGGAA ATLTATAAGI TQTAGSPSHN
GGAAGATASA GGGAAGAPPR PQQSSLAPWQ QLRDLIADAS LALYGSARPL IDGVFCADDK
EAGLVAELLA ACHSAWAAVE LDDGELRVAA ALKLALLLEE EGRLLAAREV LMQAKSVVEQ
SRVELMVANR RAPDEHLRWV TASRSQPSDD TAALVQGMTA SEQELACLQV DVLALLARVE
LGLGVSEQQG RATARRTAVM EEQAKRTAQS SIFGQRNAAE LARDEARLVA AGATPPNPQM
KERELLTACA KNPYERAVVL MQMTSFQGAD AGRKTQLLQE AGDLLVKAQA AEDGLFAAQQ
PDLRARRDVP PQPKLLQRSP TSVTLVAHPP GPGLKQPPGA RKPPSRYVAY CKSFGAGVGL
SINKTATEYP GSGVSVPLGQ PVTIQVALPC TLPQLRAPFL EEQVARLKSA RLLVLGMEVA
ALLPDEPLMQ EGALRTYGLL APLLALRAPR SHLLHKALAA CHAVLASLTN LVQDSLYRQE
HQRSLAARVA AAVTYQLLRL SDEAGEVGAA AHFGRLQLEL LKAYDPRFAL AGRPALLPGA
ELQEEASQLH DVLLQHPKLS EWAPEPLVER QKDASDLVAR VLPVLGTPAP MDTWSQALGF
ETAAEHPRWV ELVVRMVEAA VRKGNPGNAT VVAEQLTWWV RARLRRPPPP PLDAYEAEAA
EAAAAGGTAP PWPAKWKLEE AAAALDAATV AASFEPPPVP STEGMSPEEA SAALARHAAV
AAAAEATARQ RLAAVLLLQK RMPALLARKR LIEKMRENRK RWSPWMARLN LVLGLQAAAD
ARRYAANAPA RARAAAAAAE AAAAAADAPG PETSGVAGSR PGTAAVNMPP PVPDPSAAGP
PPLTPPEGIT PPLQAMMHFA RAAHLAARGG AWVEVGNAAR HAWNLARATL SADPALTAPL
PPVRWERGDA PQPAVAPESI LVPAGAGDAG GKKGSKKGGK DDKKKDDKAP AKTPSSPKGG
ARSSARSKKG FPVEEAPPPP RTYPVVVGAR PNMQRAARSL ADAVLELVSA LRDGLQVYTW
VAPVNPRHRF DGPPPRRAAL AASASTAGAG LGEEERPVTA RVTDSEFSYG SDLQADIWFK
EGPLDLAWVS RFLGLAAVVL SRGERWHALV EAGRQWARLS EGAFNERIMP WLLQAAPKAG
VDPAPFQSAM DALIRDKNQA LDQLDKVRTL VRERLGDTPL MAQSMGHKVR KRKTRAAILA
AGPGSGAGGG GGSGLPDSDR ASLVSAAYTY RTASTYKTKA SQPDFLRIPG EYEKVIEVLK
RRNEKGAMLL ALHELGDVHA HFGNWGGAAT SWNDTLDTLL GPYQALRNWR GRLDGMSPAG
TLQAYGLHGL LLGCVLAGKL ARYVHHDHLH LRLEAHRLTA RLAFCAFSAH LGHPQRRAGF
ATYTPRELWA AGTDAWLVWS DPYRCPVVDL AGALEGAAAA LLDAGLALEA LPVLALWEHV
TRHVLRNLHG TVLCRLLRVR ALVALGLLAE AVEVTGGLMG AAALPDPTLD SDYVLKDTSG
AVVEPVPAPP YDNSKLPGEP GNKAALTHIA DTPLAPAVEK LYGSWLVAHL ALARAQVLML
AGSVPNQWRG VDWRTGERTA APKPASPAKG AAKGAAAAAP EPPGAGLPEP VEPVMLERAH
VLLRKALAMA SGEDQPPSEE PSSAGKPAAA GKPAPARAPS PGRAKSPTGK GKGKGGAGDG
AGSAAAAPPE VPGPPPPPPP SASQRAEVNV RALLLMSELE QLRWMPSRGL AHALEAARFL
GEHADHVNTP QQTDNDELER YTLAPALWFL ARGAAVRCAA ALGHAAHVHE LVAAATSAHA
PSEPGSAKVP SGLPELLHCT GMAHVAALTL AAEGRTSDAL AALAAVAARY RSLCVYDGRL
AAVLLDAAAL RDRLGLREDA AELTAQGLAV AEGYCLELGL GEALEAPELT NVYLDGTALY
AHALGAAAVH ASRRQQHAEA ERCAARAVLL LRSHTRALPA THAAALLLLG RTCRMVALCG
DGVPVDGQPT LASGAPPAST ATLTSAGAGA PGDPAATAVA AGTARAAAGG FNASAGALAR
TATAGRGGGS AGSSGGGVAA TAAKLSAARS ALCASITLAA VDGGHLRSLM RGALLELGSI
FIAGLDARSA AACLRAGHAA AAKADLVALS SHTLAPVAAA QLPDWALAHV RGQEALFGKK
SSNGAISGAA GAAGSARPVA TSSSGARPPG TPPGGKPGAG GGSGDGLSDA DAARMVFCLL
GGLLKGLEAL PVGGGARARG EAQVAALHAA LRAACAKYGT DACFAEPPLP PSPPDAVPPP
PEGSVIVQWH CQDGCWQEAR SWRAEGSSGA PDGPLSDSAL LALQPVPAYA SLLFVVAAPS
HDGSPGPHCG EVTFAVKDVR ELQRRAKALR ARVEAPKAAT DILGYAAPSQ VELGELLRAA
ERLLSAVPRN SEDGSSSAGF SAADSGLGGF GSSELMEGEV RPELDVAFLC KLEALLALEA
GLDVNDKTLG SWLVQTLPVM M
