CFA61_MOUSE
ID CFA61_MOUSE Reviewed; 1252 AA.
AC Q8CEL2; Q3KNX7; Q8R1E0;
DT 04-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT 24-JUL-2007, sequence version 2.
DT 25-MAY-2022, entry version 92.
DE RecName: Full=Cilia- and flagella-associated protein 61 {ECO:0000312|MGI:MGI:1926024};
GN Name=Cfap61 {ECO:0000312|MGI:MGI:1926024};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), AND NUCLEOTIDE SEQUENCE
RP [LARGE SCALE MRNA] OF 655-1252 (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Eye;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-616 (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: May regulate cilium motility through its role in the assembly
CC of the axonemal radial spokes. {ECO:0000250|UniProtKB:A8IF44,
CC ECO:0000250|UniProtKB:Q23F13}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, cilium axoneme
CC {ECO:0000250|UniProtKB:A8IF44}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8CEL2-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8CEL2-2; Sequence=VSP_026930, VSP_026931;
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC25604.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AL672100; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL732467; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL935056; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC024760; AAH24760.1; -; mRNA.
DR EMBL; BC107030; AAI07031.1; -; mRNA.
DR EMBL; BC107031; AAI07032.1; -; mRNA.
DR EMBL; AK019712; BAC25604.1; ALT_FRAME; mRNA.
DR AlphaFoldDB; Q8CEL2; -.
DR STRING; 10090.ENSMUSP00000112099; -.
DR iPTMnet; Q8CEL2; -.
DR PhosphoSitePlus; Q8CEL2; -.
DR MaxQB; Q8CEL2; -.
DR PaxDb; Q8CEL2; -.
DR PRIDE; Q8CEL2; -.
DR ProteomicsDB; 281650; -. [Q8CEL2-1]
DR ProteomicsDB; 281651; -. [Q8CEL2-2]
DR MGI; MGI:1926024; Cfap61.
DR eggNOG; ENOG502QSEC; Eukaryota.
DR InParanoid; Q8CEL2; -.
DR ChiTaRS; Cfap61; mouse.
DR PRO; PR:Q8CEL2; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; Q8CEL2; protein.
DR GO; GO:0005930; C:axoneme; ISS:UniProtKB.
DR GO; GO:0031514; C:motile cilium; ISS:UniProtKB.
DR GO; GO:0003341; P:cilium movement; ISS:UniProtKB.
DR GO; GO:0044782; P:cilium organization; ISS:UniProtKB.
DR Gene3D; 3.50.50.60; -; 2.
DR InterPro; IPR016181; Acyl_CoA_acyltransferase.
DR InterPro; IPR038884; CFAP61.
DR InterPro; IPR032151; CFAP61_N.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR PANTHER; PTHR21178; PTHR21178; 1.
DR Pfam; PF16092; DUF4821; 1.
DR SUPFAM; SSF51905; SSF51905; 1.
DR SUPFAM; SSF55729; SSF55729; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell projection; Cytoplasm; Cytoskeleton;
KW Reference proteome.
FT CHAIN 1..1252
FT /note="Cilia- and flagella-associated protein 61"
FT /evidence="ECO:0000305"
FT /id="PRO_0000079418"
FT VAR_SEQ 235..237
FT /note="EGK -> GDR (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_026930"
FT VAR_SEQ 238..1252
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_026931"
FT CONFLICT 287..323
FT /note="EKHLKKCSTSLIIREMQIKTTLRFHLTPVRMAKIKNS -> DAESQGSPKVV
FT EEQEMAPEITQEK (in Ref. 3; BAC25604)"
FT /evidence="ECO:0000305"
FT CONFLICT 409..417
FT /note="Missing (in Ref. 3; BAC25604)"
FT /evidence="ECO:0000305"
FT CONFLICT 615..616
FT /note="LQ -> VR (in Ref. 3; BAC25604)"
FT /evidence="ECO:0000305"
FT CONFLICT 655..659
FT /note="VSKDQ -> SDPRK (in Ref. 2; AAH24760)"
FT /evidence="ECO:0000305"
FT CONFLICT 1223
FT /note="I -> L (in Ref. 2; AAH24760)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1252 AA; 143289 MW; C7C9E79DC4153CD4 CRC64;
MSILTSPRGK VEVVHCRRTE SQDIFCIKNL VRKFTQKLFG RLNIIYLLEK ANLAVTVCND
KEEIMAHSIF LDYPNWNVAK QDNWIPLFRE LDKEIPCTPL NTLFMHFFVA VDEYATGCLK
EIIRTVFKAV PELYFIFLIV PTYLSLGSTL ITVFDQVGNI PCLNYNEDFA VHICHRHNHY
PQLHIRKARV EDHDDLMPIF MHYDNTLKEI YGEYFLAELI EAQDKDHHAV VCEVEGKAVG
FMSVCTSVNL PLLHECFDLG PFHGFCTPHP DDILKPSREP SLEEGEEKHL KKCSTSLIIR
EMQIKTTLRF HLTPVRMAKI KNSETETSAS CEEALPTVLG LVSEERLKQD MEKNLSILSL
VDEEDVASSL SSSLLLSVES SHFRPLYLGD PIAFCIQLFC IDERYEARQV LISNVSLSLD
FMSFVFSLFP GKNFCLISVP HLTPEFVLIQ NFVKVVPFNN CTLDHDLYVF HRAGLLRSIT
IRLAKFLDTA GVEKLVSTLL LSTKILDDLA QYNEAGRDPD GTALQVFVAE VADQIVGIAV
IRKEMDIEYI RSHYNIEDFI YFSHHQQEEH GRLNHFALNP IFRHYTKFFL KEILRLGYKS
CLYYPVYPQT REGKLQSSYS HSLTSALHYL VPVRPRRQIV YPLEKLGINA PSKEVSKDQV
SFALNHTNRK LTLEPKVTVN ARIVVVGASS VGISFLETLV FCSHLKFNNL TLISTHGLPG
KRLLHNEQRK FLASDHCFND KDYALMSLCS WVNVVVGRMT AIDRAAKQVV VSKSEIVFYD
HLILCTGLQY QVPCPTGADT EQHLTNREVL ELSKQRYTGT VPSNLFILND EEDCVRMLCW
LRNSSILTEG KVIVYGNTLD AYTTVETLLN IGMKGSGIYF VHPPTESNIT CINNNDVESA
VEDALSAAGV TVFHDALLAQ WNHGQHPDPI YNACFTTSTK PIRLECSAFF SFYKKNVDYE
TFKAFNDACL VYDGRLVIDT TFHTNDIAIR AAGSLTKFSN RYYSNEWTHS NFSSKEIGFQ
LAAAMLSLFD PTLEPVTEPP ADLDRLIPMY KGAKIQGGIL PGSYHYLHIS KPAIPTPLNL
QRSQPDFGSD VVTGNAKKGT YFRLYINNYK LVEAITCFSK EPFPSSNYIR LFGQHEQVLN
NLCTRFDDKL IPDLYSYFTE PWCMALFHDR FIDLKKELRQ ILISKQEEDK PTMEEIAYRL
EEEEINLNEK PQKYLQRVFE DSIYKSLLEK SILDYLHYNQ YHLPMYAWPG II
