CFA61_TETTS
ID CFA61_TETTS Reviewed; 1699 AA.
AC Q23F13;
DT 12-APR-2017, integrated into UniProtKB/Swiss-Prot.
DT 18-APR-2006, sequence version 1.
DT 25-MAY-2022, entry version 59.
DE RecName: Full=Cilia- and flagella-associated protein 61 {ECO:0000305};
DE Short=CFAP61 {ECO:0000305};
DE AltName: Full=Flagellar-associated protein 61 {ECO:0000303|PubMed:25694453};
GN Name=CFAP61 {ECO:0000305}; Synonyms=FAP61 {ECO:0000303|PubMed:25694453};
GN ORFNames=TTHERM_00641200 {ECO:0000312|EMBL:EAR95092.1};
OS Tetrahymena thermophila (strain SB210).
OC Eukaryota; Sar; Alveolata; Ciliophora; Intramacronucleata;
OC Oligohymenophorea; Hymenostomatida; Tetrahymenina; Tetrahymenidae;
OC Tetrahymena.
OX NCBI_TaxID=312017;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SB210;
RX PubMed=16933976; DOI=10.1371/journal.pbio.0040286;
RA Eisen J.A., Coyne R.S., Wu M., Wu D., Thiagarajan M., Wortman J.R.,
RA Badger J.H., Ren Q., Amedeo P., Jones K.M., Tallon L.J., Delcher A.L.,
RA Salzberg S.L., Silva J.C., Haas B.J., Majoros W.H., Farzad M.,
RA Carlton J.M., Smith R.K. Jr., Garg J., Pearlman R.E., Karrer K.M., Sun L.,
RA Manning G., Elde N.C., Turkewitz A.P., Asai D.J., Wilkes D.E., Wang Y.,
RA Cai H., Collins K., Stewart B.A., Lee S.R., Wilamowska K., Weinberg Z.,
RA Ruzzo W.L., Wloga D., Gaertig J., Frankel J., Tsao C.-C., Gorovsky M.A.,
RA Keeling P.J., Waller R.F., Patron N.J., Cherry J.M., Stover N.A.,
RA Krieger C.J., del Toro C., Ryder H.F., Williamson S.C., Barbeau R.A.,
RA Hamilton E.P., Orias E.;
RT "Macronuclear genome sequence of the ciliate Tetrahymena thermophila, a
RT model eukaryote.";
RL PLoS Biol. 4:1620-1642(2006).
RN [2]
RP FUNCTION, DISRUPTION PHENOTYPE, AND SUBCELLULAR LOCATION.
RX PubMed=25694453; DOI=10.1091/mbc.e14-11-1545;
RA Urbanska P., Song K., Joachimiak E., Krzemien-Ojak L., Koprowski P.,
RA Hennessey T., Jerka-Dziadosz M., Fabczak H., Gaertig J., Nicastro D.,
RA Wloga D.;
RT "The CSC proteins FAP61 and FAP251 build the basal substructures of radial
RT spoke 3 in cilia.";
RL Mol. Biol. Cell 26:1463-1475(2015).
CC -!- FUNCTION: As component of a spoke-associated complex, regulates ciliary
CC mobility by mediating a stable and functional assembly of the radial
CC spoke 3 (RS3). {ECO:0000269|PubMed:25694453}.
CC -!- SUBCELLULAR LOCATION: Cell projection, cilium
CC {ECO:0000269|PubMed:25694453}.
CC -!- DISRUPTION PHENOTYPE: Knockout cells show deficiencies in cell
CC multiplication and ciliary functions. Mutants move with greatly reduced
CC velocities, have disturbed waveform of beating cilia and have stiffer
CC cilia (PubMed:25694453). Mutant cilia lack an adjacent portion of the
CC radial spoke 3 stem region (PubMed:25694453).
CC {ECO:0000269|PubMed:25694453}.
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DR EMBL; GG662707; EAR95092.1; -; Genomic_DNA.
DR RefSeq; XP_001015337.1; XM_001015337.1.
DR AlphaFoldDB; Q23F13; -.
DR STRING; 5911.EAR95092; -.
DR EnsemblProtists; EAR95092; EAR95092; TTHERM_00641200.
DR GeneID; 7831652; -.
DR KEGG; tet:TTHERM_00641200; -.
DR eggNOG; ENOG502QSEC; Eukaryota.
DR HOGENOM; CLU_003251_0_0_1; -.
DR InParanoid; Q23F13; -.
DR OMA; KWDFEHD; -.
DR OrthoDB; 38948at2759; -.
DR Proteomes; UP000009168; Unassembled WGS sequence.
DR GO; GO:0031514; C:motile cilium; IDA:UniProtKB.
DR GO; GO:0003341; P:cilium movement; IMP:UniProtKB.
DR GO; GO:0044782; P:cilium organization; IMP:UniProtKB.
DR InterPro; IPR038884; CFAP61.
DR InterPro; IPR032151; CFAP61_N.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR PANTHER; PTHR21178; PTHR21178; 3.
DR Pfam; PF16092; DUF4821; 1.
DR SUPFAM; SSF51905; SSF51905; 1.
PE 4: Predicted;
KW Cell projection; Coiled coil; Reference proteome.
FT CHAIN 1..1699
FT /note="Cilia- and flagella-associated protein 61"
FT /id="PRO_0000439531"
FT REGION 1..23
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 489..515
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1340..1365
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 500..515
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1699 AA; 198392 MW; EA5CF8CACE46C33C CRC64;
MYSNNQLDNP NHSRSQYRNG DQSGIYAGSD EEFQVLVRKS DLDDVDEVLK IIAQHEREAN
DLLYDYPNLL SLFEKNYLSV TILNQQQQII GAAVFNDFPQ GLTGQIDFQH ENFWENWIFD
AFNFDESICI TPFNTLWMTF FFVAKDQYKF SQEEELIICQ KVFQNVYATL PQLEGILFLK
RGETMEDNGA QAQISSILEN IFIEVPVKDR DIIKRVRGIH LNSEVFYSPK HACQEIIEIR
MAREQDHDDL AEIFNRQSEV LTAQFGEFFI ADLIATQNLT RRGTRKFLNN GKAIVGQVRD
KAVGLMSIST DIDYKFLAQC FELETYDNLF KPDFMEAIQN KRDDIRRENQ YREEDQYQEI
VKKMNEERMK SHFIGQRMTL QQHCREKEQE IVMKIDEIIN NDAIQKNLTK KQVEEFLDEW
LRGYELHQPS DLFYQYPHIF PNTQSIILTP RQLLIETLEF FGLPQHYMNG AGHWKDWVQK
KMEEKRIAQL KRPQKKVTKR PKRQKEEDKK EDEFKPPSYF DLTPFQSAFK KFLAVNPEGR
TIFRQKIEAS MKVIKMMFCN ENGEMKEERH VDLNDLGNEL NKQKMDIAPE VCENLASFLE
CFGEIKYEEE IIMVNPPKED TNQNAKIAQT KKMFASEQTE SKPIPKLLKK TSFQEFFDAV
YKMKEFDIMM NRLDITKSTT LKAEVSQIVK EEAQQEKAKQ EKRRIERSPN PYDDYVKNLK
DLDSIPEVPI DAQNAIVINL FCIDENFESR SLDFVEYAFS IFGDRDYIIL TQPFTVPETT
LLQQFIKIPM KKNSTFDHVL YIYHKDCLLS NTLFIRKSKL EDMQYVAPLF SNLINKQQIY
DDTLEAITQT ASRKVAFSVF CDQSLIGIYV VSKNVNLDYY ISHFCVQDHI ILKEHPMTCH
TKLIHTALNP LFTKNTRFIL KEILRLMNKT CMYFEVQEKT LLPDVFNELV FVRARQFPHF
LKRKWDFEHD KEHFERAGDL TEVQDGKRDA FDQEQAPFSL SVITKKMMSS VKVNNNTRIV
VVGASDTGIS FIESLLSVKD INFTHITLLA PGGIMTMHIN LPGDQLKALS TNYTLEELKN
LMLDARVNVL DAKMVELDKK GKKIKLDKNA ELPYDYLVNT VGLIDTELQN RRLISTGLFS
SPYYQQLLDK YGRDRAQIEE GAPPIFGQHP VYGNYVQGVY SIDDPYLYEE FKKTGKKDSN
IDLLTRKKRP QSITIYGRTL HTIAFISGMV NRGVHPNRIH YVIPPKVFET QVQFKNNTER
LIYEDKRIND PDPFDDPSVE QKIFEFMESK GIQVHKGYNL HQIDVDEETR ICQGVVFRKK
ADNYEEIIQQ IEIKKQQILE RDANSENADK GFDDTQSRDG DEENQPITLE QEIEELERNK
FDDIEITSRF LVTSGLVDID KEIFYIIHEN GLVYNGRLIV KSNFQTTEKD IFCCGKICEF
SQRYKRLSIG RSLRLDKYNG RELGQKLAKC LLDSLDLGYL TDQIYSLGEL PNLYMPIGIG
AFLPGDLFYY HIKKNDYARP SKTMEAEDNR QDIVSDNIDQ KQQIGHYIKF KFDNNGIIDQ
VTYLGTEPVS VQSLVSFVGL SEKCLNQLDV RFKNGLIPNI SEFLSENWAI GLYHEWFSEF
RHITKMEMLQ SENIQQILDE AAKYAQGGGF LDPEFIEKIK AKISPEIISQ IQQGTLDFIR
ENQNHLPMYY VPPKKVNVD
