CFA65_CHLRE
ID CFA65_CHLRE Reviewed; 2118 AA.
AC A8JFU2;
DT 05-OCT-2016, integrated into UniProtKB/Swiss-Prot.
DT 04-DEC-2007, sequence version 1.
DT 25-MAY-2022, entry version 57.
DE RecName: Full=Cilia- and flagella-associated protein 65 {ECO:0000305};
DE Flags: Fragment;
GN Name=CFAP65 {ECO:0000250|UniProtKB:Q6ZU64};
GN Synonyms=FAP65 {ECO:0000303|PubMed:15998802};
GN ORFNames=CHLREDRAFT_153955 {ECO:0000312|EMBL:EDO97267.1};
OS Chlamydomonas reinhardtii (Chlamydomonas smithii).
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX NCBI_TaxID=3055;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CC-503;
RX PubMed=17932292; DOI=10.1126/science.1143609;
RA Merchant S.S., Prochnik S.E., Vallon O., Harris E.H., Karpowicz S.J.,
RA Witman G.B., Terry A., Salamov A., Fritz-Laylin L.K., Marechal-Drouard L.,
RA Marshall W.F., Qu L.H., Nelson D.R., Sanderfoot A.A., Spalding M.H.,
RA Kapitonov V.V., Ren Q., Ferris P., Lindquist E., Shapiro H., Lucas S.M.,
RA Grimwood J., Schmutz J., Cardol P., Cerutti H., Chanfreau G., Chen C.L.,
RA Cognat V., Croft M.T., Dent R., Dutcher S., Fernandez E., Fukuzawa H.,
RA Gonzalez-Ballester D., Gonzalez-Halphen D., Hallmann A., Hanikenne M.,
RA Hippler M., Inwood W., Jabbari K., Kalanon M., Kuras R., Lefebvre P.A.,
RA Lemaire S.D., Lobanov A.V., Lohr M., Manuell A., Meier I., Mets L.,
RA Mittag M., Mittelmeier T., Moroney J.V., Moseley J., Napoli C.,
RA Nedelcu A.M., Niyogi K., Novoselov S.V., Paulsen I.T., Pazour G.J.,
RA Purton S., Ral J.P., Riano-Pachon D.M., Riekhof W., Rymarquis L.,
RA Schroda M., Stern D., Umen J., Willows R., Wilson N., Zimmer S.L.,
RA Allmer J., Balk J., Bisova K., Chen C.J., Elias M., Gendler K., Hauser C.,
RA Lamb M.R., Ledford H., Long J.C., Minagawa J., Page M.D., Pan J.,
RA Pootakham W., Roje S., Rose A., Stahlberg E., Terauchi A.M., Yang P.,
RA Ball S., Bowler C., Dieckmann C.L., Gladyshev V.N., Green P., Jorgensen R.,
RA Mayfield S., Mueller-Roeber B., Rajamani S., Sayre R.T., Brokstein P.,
RA Dubchak I., Goodstein D., Hornick L., Huang Y.W., Jhaveri J., Luo Y.,
RA Martinez D., Ngau W.C., Otillar B., Poliakov A., Porter A., Szajkowski L.,
RA Werner G., Zhou K., Grigoriev I.V., Rokhsar D.S., Grossman A.R.;
RT "The Chlamydomonas genome reveals the evolution of key animal and plant
RT functions.";
RL Science 318:245-250(2007).
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RX PubMed=15998802; DOI=10.1083/jcb.200504008;
RA Pazour G.J., Agrin N., Leszyk J., Witman G.B.;
RT "Proteomic analysis of a eukaryotic cilium.";
RL J. Cell Biol. 170:103-113(2005).
CC -!- FUNCTION: May play a role in flagellar formation and mobility.
CC {ECO:0000250|UniProtKB:Q3V0B4}.
CC -!- SUBCELLULAR LOCATION: Cell projection, cilium, flagellum membrane
CC {ECO:0000269|PubMed:15998802}; Single-pass membrane protein
CC {ECO:0000255}. Cytoplasm {ECO:0000250|UniProtKB:Q3V0B4}.
CC -!- SIMILARITY: Belongs to the CFAP65 family. {ECO:0000305}.
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DR EMBL; DS496178; EDO97267.1; -; Genomic_DNA.
DR RefSeq; XP_001702074.1; XM_001702022.1.
DR AlphaFoldDB; A8JFU2; -.
DR STRING; 3055.EDO97267; -.
DR PaxDb; A8JFU2; -.
DR PRIDE; A8JFU2; -.
DR EnsemblPlants; PNW81405; PNW81405; CHLRE_07g354551v5.
DR GeneID; 5727614; -.
DR Gramene; PNW81405; PNW81405; CHLRE_07g354551v5.
DR eggNOG; ENOG502QSJW; Eukaryota.
DR HOGENOM; CLU_232155_0_0_1; -.
DR InParanoid; A8JFU2; -.
DR OrthoDB; 87636at2759; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0031514; C:motile cilium; IDA:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-KW.
DR GO; GO:0030030; P:cell projection organization; IEA:UniProtKB-KW.
DR Gene3D; 2.60.40.10; -; 10.
DR InterPro; IPR013783; Ig-like_fold.
PE 1: Evidence at protein level;
KW Cell membrane; Cell projection; Cilium; Cilium biogenesis/degradation;
KW Coiled coil; Cytoplasm; Flagellum; Membrane; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..2118
FT /note="Cilia- and flagella-associated protein 65"
FT /id="PRO_5002722409"
FT TRANSMEM 6..26
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 615..736
FT /note="MSP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00132"
FT REGION 1007..1029
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1764..1909
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1924..1958
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 2016..2045
FT /evidence="ECO:0000255"
FT NON_TER 2118
SQ SEQUENCE 2118 AA; 220136 MW; EBE3014374EC5C07 CRC64;
MSSSRGSLRA LLLAAAAAAA AAAGAVSNKL GYWFSCRRFG LDCPEQLEWR HWNPGTEYVK
QLVLKNVSTS VLKIRFKQPT SKAFGMDFPE PFKLRPGMSQ PLKVVFRPLK QQHYSDNVEL
FVGNVSCLVP VHAYTPVTHI EVPPALDFGF TPTKETVTAQ LPVRNTGDVR VDVLWKLDAP
FSISPLFASL APGEVVAFTA SFTPPEACSY TASAACQLES GAAAICKISG IGKFPYLSVE
QAGVDFGAVV VGQRVERLVR FGNHSVVPAH FAVTHDEAGP DDGVFTVGPA RGTLGPEEYS
MLKLSYTPRA TGTFSSETFH IGTAGGNRVA LNLRGTAVAP LVTLSTRAFN FGNVAAGATA
SRVLYIRNHS AVPVPYDFQV DPLDVFAISR TRGVLAPDST AHVTITFRPM AGLAANLWRR
VTLLLKDAEP QGVDLVATAY TDKSRPPPLS QRHVERYMAR VLSGGPAVEE SQLDSKPPSA
ATGDPAAALT VDSDALDFGS CSRLSASEYR SVTVTNHTPA KLTAFVLVPE WADPGAAPGD
APQRVFQVFP ESADMRPYGQ ATFKVAFRPP RDAAFFSQQL ALVAHTKSQR NFRLVAEHQV
LPAWCVPLRA TGNTFLHSNP EFGPKVELSS RAVAFPPCRP GERVHQTLLI ANSGDTPVAF
SFGAAGAALG PLFSAKPAAG VVPPQGHVLV ALRFAPTDTR PAAANALVTF NGVASNAVSV
SLRGAAHTPR LTTDLPSSTL YFRPTCVGAS SQRAVTLHNP SRVPVAFRWR LPARLQGLVT
VSPAAGALRG NESVQLQWSF TPAAQKLYEA RAACLVMSPP EAANGNAAGA TASAASLFPG
AGLATGIEWY EGAGATAALA GMGEEEAADA VLLSLVGEGT QGAVALEPPS LELGDLRVGH
PVRRPLLLQN CSDGVLRYSL EVGPAEDDQP PAGDATLVDF TAGLDSAAEG GVGGGAGGAG
GAALECWVDE PEGALPARAS KTVMVTLFPR YRKRYSLQVR CRSSTVAPLL TGPNRPPSGG
AGPRPLDAGS EAAAAAAAAA AAAAAPLPPP VIAPLTASTT FPTLEVTDVY CEGLPKQLLW
QLLGLNDLNH HLRTEVTATE LRLRAAQDRG ALTTEAASAA MRPFLMEFGT HGLGGRPRVV
HVEISNPTPL PASWQLHSFD DPDGVELENW VEPGRPRTEG ERMRDLIAEY KLFEMRPRSG
ELEPGARCTV TIEFRPSVEG SFELPVFLHI TDGKRLRLQL QAVTTPEPLQ LLALPPPLRT
FRLEPVALGE RAPPLQMYVL RNGGPAPLHW RLDTAPLAAL AEASWGHPVL ELVGPEEGDI
EEGGVAAINW RFSPLEAKEY RVEVPVLLGD GGIEVIELLG RGFAPPPAPP HGAAAVQLIG
PGAATSPAAP TTAAEDDDTP AAALDSVGGD AEAEAARAAA AADRDWITWR GLSSAPSAGM
AGRLALVDHD LVSLGVTPVR GLTRRIIVLT NKSRYPLAFD WDLGCLAPPP PPGLAATQRA
AAASAMMGLG GGSLLPASQL QLLAGRPLQG ALAISPAAGS LEPGERLVCR VSLHAGVTPQ
VFEGEVRCHV RIDDDAVAEA EAAAAAAAAA GAAAVAAELP FVEQVEEVIA EAPVRGPPAP
PPAVAAAQRA SRLRSRLPVH QYMTTAVRTR IEPLNAAFTA TMEARTRRLA DATRPPSWPE
PQSISVTLRG RILDERQLGA LRYVPPHERA AARAAVVAGA AWVPPAMVPF WEEEGRSTPA
VSAQPSSQGG GGYGVGGYGG GYGSGGSQGG EASVAPSGGD PGGYSPRGGM ADSWPSLEPG
LGMEETVVGP PTVGLDLSLS LQHAGGAGGA PGGDGDSDSR PGTPSMTAAA HHHHHHPRHA
GPAPGSGTAE VMEVTFSGDQ QPTGGPMPSP PSISGAPDPD SATPDDDFAT SDAAVNAAAA
VRAAGGGMGS PQPVDPGGSA AAAASELAPP ALPPAPPRGG DIEYDALVSA REVLAGLFAE
LMEDADVVRA VAFLPEHEVP SFAEVRATPP PCYDPAVRAA AEAARAEAEA RAAAEAATKA
AAEAEAGAAF AIPAAAAQAA TEPPALEDEL AADTELARVL VAPEWRAFAD FVLESAVLGL
MQESAAGDWE MPEEEVGR