CFA70_CHLRE
ID CFA70_CHLRE Reviewed; 1104 AA.
AC A8ITV9;
DT 04-MAR-2015, integrated into UniProtKB/Swiss-Prot.
DT 04-DEC-2007, sequence version 1.
DT 03-AUG-2022, entry version 56.
DE RecName: Full=Cilia- and flagella-associated protein 70 {ECO:0000305};
DE AltName: Full=Flagellum-associated protein 70 {ECO:0000305};
GN Name=CFAP70 {ECO:0000305}; Synonyms=FAP70 {ECO:0000312|EMBL:EDP04030.1};
GN ORFNames=CHLREDRAFT_12006 {ECO:0000312|EMBL:EDP04030.1};
OS Chlamydomonas reinhardtii (Chlamydomonas smithii).
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX NCBI_TaxID=3055;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CC-503;
RX PubMed=17932292; DOI=10.1126/science.1143609;
RA Merchant S.S., Prochnik S.E., Vallon O., Harris E.H., Karpowicz S.J.,
RA Witman G.B., Terry A., Salamov A., Fritz-Laylin L.K., Marechal-Drouard L.,
RA Marshall W.F., Qu L.H., Nelson D.R., Sanderfoot A.A., Spalding M.H.,
RA Kapitonov V.V., Ren Q., Ferris P., Lindquist E., Shapiro H., Lucas S.M.,
RA Grimwood J., Schmutz J., Cardol P., Cerutti H., Chanfreau G., Chen C.L.,
RA Cognat V., Croft M.T., Dent R., Dutcher S., Fernandez E., Fukuzawa H.,
RA Gonzalez-Ballester D., Gonzalez-Halphen D., Hallmann A., Hanikenne M.,
RA Hippler M., Inwood W., Jabbari K., Kalanon M., Kuras R., Lefebvre P.A.,
RA Lemaire S.D., Lobanov A.V., Lohr M., Manuell A., Meier I., Mets L.,
RA Mittag M., Mittelmeier T., Moroney J.V., Moseley J., Napoli C.,
RA Nedelcu A.M., Niyogi K., Novoselov S.V., Paulsen I.T., Pazour G.J.,
RA Purton S., Ral J.P., Riano-Pachon D.M., Riekhof W., Rymarquis L.,
RA Schroda M., Stern D., Umen J., Willows R., Wilson N., Zimmer S.L.,
RA Allmer J., Balk J., Bisova K., Chen C.J., Elias M., Gendler K., Hauser C.,
RA Lamb M.R., Ledford H., Long J.C., Minagawa J., Page M.D., Pan J.,
RA Pootakham W., Roje S., Rose A., Stahlberg E., Terauchi A.M., Yang P.,
RA Ball S., Bowler C., Dieckmann C.L., Gladyshev V.N., Green P., Jorgensen R.,
RA Mayfield S., Mueller-Roeber B., Rajamani S., Sayre R.T., Brokstein P.,
RA Dubchak I., Goodstein D., Hornick L., Huang Y.W., Jhaveri J., Luo Y.,
RA Martinez D., Ngau W.C., Otillar B., Poliakov A., Porter A., Szajkowski L.,
RA Werner G., Zhou K., Grigoriev I.V., Rokhsar D.S., Grossman A.R.;
RT "The Chlamydomonas genome reveals the evolution of key animal and plant
RT functions.";
RL Science 318:245-250(2007).
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RX PubMed=15998802; DOI=10.1083/jcb.200504008;
RA Pazour G.J., Agrin N., Leszyk J., Witman G.B.;
RT "Proteomic analysis of a eukaryotic cilium.";
RL J. Cell Biol. 170:103-113(2005).
RN [3]
RP FUNCTION, DISRUPTION PHENOTYPE, AND SUBCELLULAR LOCATION.
RX PubMed=30158508; DOI=10.3390/cells7090124;
RA Shamoto N., Narita K., Kubo T., Oda T., Takeda S.;
RT "CFAP70 Is a Novel Axoneme-Binding Protein That Localizes at the Base of
RT the Outer Dynein Arm and Regulates Ciliary Motility.";
RL Cells 7:E124-E124(2018).
CC -!- FUNCTION: Axoneme-binding protein that acts as a regulatory componement
CC of the outer dynein arm and regulates ciliary motility.
CC {ECO:0000269|PubMed:30158508}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, cilium axoneme
CC {ECO:0000269|PubMed:30158508}. Cell projection, cilium
CC {ECO:0000269|PubMed:15998802}. Note=Localizes at the base of outer
CC dynein arms. {ECO:0000269|PubMed:30158508}.
CC -!- DISRUPTION PHENOTYPE: Impaired motility with outer dynein arm defects.
CC {ECO:0000269|PubMed:30158508}.
CC -!- SIMILARITY: Belongs to the CFAP70 family. {ECO:0000305}.
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DR EMBL; DS496123; EDP04030.1; -; Genomic_DNA.
DR RefSeq; XP_001692552.1; XM_001692500.1.
DR AlphaFoldDB; A8ITV9; -.
DR STRING; 3055.EDP04030; -.
DR PaxDb; A8ITV9; -.
DR PRIDE; A8ITV9; -.
DR GeneID; 5718018; -.
DR eggNOG; ENOG502QSJ2; Eukaryota.
DR HOGENOM; CLU_290911_0_0_1; -.
DR InParanoid; A8ITV9; -.
DR OrthoDB; 989532at2759; -.
DR GO; GO:0005930; C:axoneme; IDA:UniProtKB.
DR GO; GO:0005929; C:cilium; IDA:UniProtKB.
DR GO; GO:0036157; C:outer dynein arm; IMP:UniProtKB.
DR GO; GO:0003341; P:cilium movement; IMP:UniProtKB.
DR Gene3D; 1.25.40.10; -; 2.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR SUPFAM; SSF48452; SSF48452; 2.
DR PROSITE; PS50005; TPR; 1.
DR PROSITE; PS50293; TPR_REGION; 2.
PE 1: Evidence at protein level;
KW Cell projection; Cytoplasm; Cytoskeleton; Repeat; TPR repeat.
FT CHAIN 1..1104
FT /note="Cilia- and flagella-associated protein 70"
FT /id="PRO_0000432113"
FT REPEAT 583..618
FT /note="TPR 1"
FT /evidence="ECO:0000255"
FT REPEAT 846..879
FT /note="TPR 2"
FT /evidence="ECO:0000255"
FT REPEAT 917..950
FT /note="TPR 3"
FT /evidence="ECO:0000255"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 400..419
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 706..737
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 878..900
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 400..415
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 880..900
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1104 AA; 114068 MW; 4B9ABE5E5F92CEF3 CRC64;
MAPKKKEDKD APPPEDTNPY GVFVSVTMPS ATVVLDVQDD AAAQRETHIT LNIPGCHVPW
ASAHTAPSEG GVYQYSVVKH FRRSGGQDGL LQLINGVLTV TVNDSATNAV LASATVDQLQ
GFAIGQNTWS TEGLDLVPAA EVPEGVPKAR SARLAFASIA LQERPLPEGA DAAALAAAPP
PPEAELPPLL PYSYVSGEAA EKGNMVELVV SGLSPLPPNL QAAADAAGGK LHVTVGLALP
GGGPAVAVSL AVAGGRVAAP AFRRELLPPG CLQALQYALE DGLPVVLEVA RYVSAEGMAD
PAFEGYHAAA AAPALAAGLG AAGATEAAAE GLSLTAWAAS GAKTCLPPYT APAGKPKPVE
AEVPPAGTCL WEKAGAQLAV SLRFARPVVP AWRPPPPPPR PLLELIPPRD LTPKPPPTTA
VDEFKAKVRV IARALAEEYK AVLPPPDASV AAAAAGGGAE GRHKALIFEL NRSGKYAQMR
DSLKTAVVSL VREKYRKSGS MSPNEMALLY NDLYGSLLAA VHSSLNDLVD AAAARPRAPP
PAPVPDKQRL GELLELAAQA EAMGDTDRAE LLHQRRLLAK NDAQVWYEYG TYCLRRGGAK
RGRAEECFRE ALALEPAHRG ALLALLGCSV AAGRNTDPAY LESAEAAAHR LLDVAGRSSL
DAWAALAVVY RAYGEAKRAE LASCEQEMAR LEKQQLAAAA AAASAGVSPS PSYGEGRAGG
NGGGVPATEP STASAGSVAG SAGELQARSF ISLANTLLES LALPAEAALA LELAAGLRHW
PSVGPDTRTL HALAGALAEQ ALARAAGGGA ASAAAEAMLT PGSSVLSMMR ADAGEAVSSV
AAEAAWRCRL LVAQLHKARG ATDEAIRFYQ SFIAITQQHA PSTPPPPPPP PPPPPPPPPQ
EYIEAARSSG RLAEVPLSAW LELAEAYAAR GQARFAADVF LLGASARPGC AVLWRGAGRC
FVGAEELGPA DMALSEANVL DPEDPEAWGW LALVALREGR AEDAEKALAF GLRCGLGDPG
LLLDIAAEYR AAGQRRAEQR VLQEVAVKLM PESCSARLLL ARCLVAQRCG AEAAEAVAAA
RQLAAHEDDE AAVAELEAEL RGMA
