CFA70_MOUSE
ID CFA70_MOUSE Reviewed; 1141 AA.
AC D3YVL2; B7ZNG2; B7ZNG3; D3Z1K9; E9Q7X8;
DT 22-APR-2020, integrated into UniProtKB/Swiss-Prot.
DT 05-APR-2011, sequence version 2.
DT 03-AUG-2022, entry version 84.
DE RecName: Full=Cilia- and flagella-associated protein 70;
DE AltName: Full=Tetratricopeptide repeat protein 18;
DE Short=TPR repeat protein 18;
GN Name=Cfap70 {ECO:0000312|MGI:MGI:1923920}; Synonyms=Ttc18;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [4]
RP TISSUE SPECIFICITY, SUBCELLULAR LOCATION, AND FUNCTION.
RX PubMed=30158508; DOI=10.3390/cells7090124;
RA Shamoto N., Narita K., Kubo T., Oda T., Takeda S.;
RT "CFAP70 Is a Novel Axoneme-Binding Protein That Localizes at the Base of
RT the Outer Dynein Arm and Regulates Ciliary Motility.";
RL Cells 7:E124-E124(2018).
CC -!- FUNCTION: Axoneme-binding protein that plays a role in the regulation
CC of ciliary motility and cilium length. {ECO:0000269|PubMed:30158508}.
CC -!- SUBCELLULAR LOCATION: Cell projection, cilium, flagellum
CC {ECO:0000269|PubMed:30158508}. Cytoplasm, cytoskeleton, flagellum basal
CC body {ECO:0000250|UniProtKB:Q5T0N1}. Cell projection, cilium
CC {ECO:0000269|PubMed:30158508}. Cytoplasm, cytoskeleton, cilium axoneme
CC {ECO:0000269|PubMed:30158508}. Note=Present all along the flagellum,
CC with a marked signal at the base of the flagellum.
CC {ECO:0000250|UniProtKB:Q5T0N1}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=D3YVL2-1; Sequence=Displayed;
CC Name=2;
CC IsoId=D3YVL2-2; Sequence=VSP_060550;
CC Name=3;
CC IsoId=D3YVL2-3; Sequence=VSP_060549, VSP_060551;
CC -!- TISSUE SPECIFICITY: Detected in ependyma, trachea, lung, testis, and
CC oviduct, but not in whole brain, liver, kidney, and spleen
CC (PubMed:30158508). Localizes on the epithelial cilia and sperm flagella
CC (at protein level) (PubMed:30158508). {ECO:0000269|PubMed:30158508}.
CC -!- DOMAIN: The conserved TPR domains are dispensable for ciliary
CC targeting. The N-terminal half is important for cilary localization
CC and/or binding to the axoneme. {ECO:0000269|PubMed:30158508}.
CC -!- SIMILARITY: Belongs to the CFAP70 family. {ECO:0000305}.
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DR EMBL; AC121801; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC138269; AAI38270.1; -; mRNA.
DR EMBL; BC145226; AAI45227.1; -; mRNA.
DR EMBL; BC145225; AAI45226.1; -; mRNA.
DR CCDS; CCDS49411.1; -. [D3YVL2-3]
DR CCDS; CCDS49412.1; -. [D3YVL2-2]
DR CCDS; CCDS49413.1; -. [D3YVL2-1]
DR RefSeq; NP_001157110.1; NM_001163638.1. [D3YVL2-3]
DR RefSeq; NP_001157111.1; NM_001163639.1. [D3YVL2-2]
DR RefSeq; NP_083974.1; NM_029698.1. [D3YVL2-1]
DR RefSeq; XP_006519744.1; XM_006519681.1.
DR RefSeq; XP_006519745.1; XM_006519682.1.
DR RefSeq; XP_006519746.1; XM_006519683.1.
DR AlphaFoldDB; D3YVL2; -.
DR STRING; 10090.ENSMUSP00000116668; -.
DR PhosphoSitePlus; D3YVL2; -.
DR MaxQB; D3YVL2; -.
DR PaxDb; D3YVL2; -.
DR ProteomicsDB; 312216; -.
DR ProteomicsDB; 355454; -.
DR ProteomicsDB; 363635; -. [D3YVL2-1]
DR Antibodypedia; 29381; 18 antibodies from 8 providers.
DR Ensembl; ENSMUST00000022348; ENSMUSP00000022348; ENSMUSG00000039543. [D3YVL2-2]
DR Ensembl; ENSMUST00000022349; ENSMUSP00000022349; ENSMUSG00000039543. [D3YVL2-3]
DR Ensembl; ENSMUST00000056073; ENSMUSP00000056869; ENSMUSG00000039543. [D3YVL2-1]
DR GeneID; 76670; -.
DR KEGG; mmu:76670; -.
DR UCSC; uc007sjq.2; mouse. [D3YVL2-1]
DR CTD; 118491; -.
DR MGI; MGI:1923920; Cfap70.
DR VEuPathDB; HostDB:ENSMUSG00000039543; -.
DR eggNOG; ENOG502QSJ2; Eukaryota.
DR GeneTree; ENSGT00390000013319; -.
DR HOGENOM; CLU_009208_0_0_1; -.
DR OMA; MSDYHMQ; -.
DR OrthoDB; 260340at2759; -.
DR BioGRID-ORCS; 76670; 4 hits in 72 CRISPR screens.
DR ChiTaRS; Cfap70; mouse.
DR PRO; PR:D3YVL2; -.
DR Proteomes; UP000000589; Chromosome 14.
DR RNAct; D3YVL2; protein.
DR Bgee; ENSMUSG00000039543; Expressed in spermatocyte and 85 other tissues.
DR Genevisible; D3YVL2; MM.
DR GO; GO:0005930; C:axoneme; IDA:UniProtKB.
DR GO; GO:0036064; C:ciliary basal body; ISO:MGI.
DR GO; GO:0031514; C:motile cilium; IDA:UniProtKB.
DR GO; GO:0036157; C:outer dynein arm; ISS:UniProtKB.
DR GO; GO:0036126; C:sperm flagellum; IDA:UniProtKB.
DR GO; GO:0060271; P:cilium assembly; IMP:UniProtKB.
DR GO; GO:0003341; P:cilium movement; IMP:UniProtKB.
DR Gene3D; 1.25.40.10; -; 2.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR InterPro; IPR019734; TPR_repeat.
DR Pfam; PF13181; TPR_8; 2.
DR SMART; SM00028; TPR; 8.
DR SUPFAM; SSF48452; SSF48452; 2.
DR PROSITE; PS50005; TPR; 6.
DR PROSITE; PS50293; TPR_REGION; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell projection; Cilium; Cytoplasm; Cytoskeleton;
KW Flagellum; Reference proteome; Repeat; TPR repeat.
FT CHAIN 1..1141
FT /note="Cilia- and flagella-associated protein 70"
FT /id="PRO_0000449347"
FT REPEAT 634..667
FT /note="TPR 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00339"
FT REPEAT 668..701
FT /note="TPR 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00339"
FT REPEAT 702..735
FT /note="TPR 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00339"
FT REPEAT 949..982
FT /note="TPR 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00339"
FT REPEAT 983..1016
FT /note="TPR 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00339"
FT REPEAT 1020..1053
FT /note="TPR 6"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00339"
FT REPEAT 1055..1086
FT /note="TPR 7"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00339"
FT REPEAT 1087..1120
FT /note="TPR 8"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00339"
FT REGION 392..448
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 811..874
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 392..406
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 407..426
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 432..448
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 811..846
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 855..874
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 375..430
FT /note="Missing (in isoform 3)"
FT /id="VSP_060549"
FT VAR_SEQ 574..617
FT /note="Missing (in isoform 2)"
FT /id="VSP_060550"
FT VAR_SEQ 814
FT /note="G -> GEIPCLFITHHLGPGLKCLLILIFISYRIYSLHKYSLKTG (in
FT isoform 3)"
FT /id="VSP_060551"
FT CONFLICT 10
FT /note="I -> T (in Ref. 2; AAI38270/AAI45227/AAI45226)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1141 AA; 128276 MW; E9163A8DD6E0C977 CRC64;
MDQTSSTTKI VHITVTNGYD LKGFKGDTPV TFVRAEFNQT VLGDSSKVTV SPEGTAKYNF
TSNIDLSPDG GGALDDLAHK PLFLTVTEVL PKEKKQKDEK TLILGQAVVD LLPLLEGEES
FETTVPLHPV PGSPLETPLP GSKQCSLDVK VFVAEPLLTP AQVSASNLLK VTLEAAYSVP
ESFIPVGPQQ NYMVGLQVPS VGEKDYTMIF KNGNLKLGGE KEPVPRPKKW PIANILAPGA
SNIPDEFIVG GPYEEEEGEL NHPEDREFRN QAECTKKRIV WDLESRCFLH PFAVASFQKR
IADCRLWPVE ITRVPLVVMP KAKPGKLEKI DDENQLSFHG VAYINMVPLL YPGVKKIRGA
FHVYPYLDGT VFEKTKCLFS LFRDTGHHLV QNNKAGGLNS PLSKPLSKNL KEEKPGKDKD
TEGRPRLGEL QAPSIKSQSS DTPLESEAPL SHNLEGQQYI EAGTYIVLEI QLEKALVPKR
MPEELARRVK EMIPPRPPLT RRTGGAQKAV SDYHTQIKSI SRAILNEYYR MFGKQGPKLE
SDIDNETMEE RKCQLNYELN CSGKYFAFKE QLKHAVVKIV REKYLKTTAF ESQEELQTFI
SELYVFLVDQ MHVALNQAVP DDVPSSTSTI QTSSEQLRLF AFEAEVNEKF EIAAMYYEER
LVREPQNLEN WLDYGAFCLL TEDNIKAQEC FRKALSLNES HVDSLLLCGV LAILLENYEQ
AEIFFEDATC LEPTNVIAWT LLGLFYEIQN NDIRMEMAFH EAFKQLQART LQTKLKSTVT
IENMEEGVKV EPSFGPWGVV QESTTAIKTE GLSGMRPQSS HQLSPHTNME LHPQPQGPNT
ALSSLDEFLE ESPKAQSESQ EPMATGQPLE PSLVQRSSNA LLKELTSKKD ISKCQDSSAF
SPPTQHVIAQ PPVTIFMETI RFLMKVNAVQ FVHRVLAHEL LCPQGGPSCE YYLVLAQTHL
LKKDFAKTEE YLQQAAQMDY LNPNVWGVKG HLYFLSGNHA EAKECYERTI SFVVDASEMH
FIFLRLGHIY LEEKEYENAK RTYMHACKRS PSCLTWLGLG IACYRLEELT EAEDALSEAN
ALNNCNAEVW AYLALVCLKV GRQLEAEQAY KYTIKLKLKD QALLEEIHTV QEMVGFGNPS
F
