CFA74_CHLRE
ID CFA74_CHLRE Reviewed; 1940 AA.
AC D4P3R7; A8HVZ2;
DT 04-FEB-2015, integrated into UniProtKB/Swiss-Prot.
DT 18-MAY-2010, sequence version 1.
DT 03-AUG-2022, entry version 45.
DE RecName: Full=Cilia- and flagella-associated protein 74 {ECO:0000305};
DE AltName: Full=Flagella-associated protein 74 {ECO:0000303|PubMed:20421426};
GN Name=CFAP74 {ECO:0000305}; Synonyms=FAP74 {ECO:0000303|PubMed:20421426};
GN ORFNames=CHLREDRAFT_167096 {ECO:0000312|EMBL:EDP08468.1};
OS Chlamydomonas reinhardtii (Chlamydomonas smithii).
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX NCBI_TaxID=3055;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBUNIT, AND SUBCELLULAR LOCATION.
RX PubMed=20421426; DOI=10.1083/jcb.200912009;
RA DiPetrillo C.G., Smith E.F.;
RT "Pcdp1 is a central apparatus protein that binds Ca2+-calmodulin and
RT regulates ciliary motility.";
RL J. Cell Biol. 189:601-612(2010).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CC-503;
RX PubMed=17932292; DOI=10.1126/science.1143609;
RA Merchant S.S., Prochnik S.E., Vallon O., Harris E.H., Karpowicz S.J.,
RA Witman G.B., Terry A., Salamov A., Fritz-Laylin L.K., Marechal-Drouard L.,
RA Marshall W.F., Qu L.H., Nelson D.R., Sanderfoot A.A., Spalding M.H.,
RA Kapitonov V.V., Ren Q., Ferris P., Lindquist E., Shapiro H., Lucas S.M.,
RA Grimwood J., Schmutz J., Cardol P., Cerutti H., Chanfreau G., Chen C.L.,
RA Cognat V., Croft M.T., Dent R., Dutcher S., Fernandez E., Fukuzawa H.,
RA Gonzalez-Ballester D., Gonzalez-Halphen D., Hallmann A., Hanikenne M.,
RA Hippler M., Inwood W., Jabbari K., Kalanon M., Kuras R., Lefebvre P.A.,
RA Lemaire S.D., Lobanov A.V., Lohr M., Manuell A., Meier I., Mets L.,
RA Mittag M., Mittelmeier T., Moroney J.V., Moseley J., Napoli C.,
RA Nedelcu A.M., Niyogi K., Novoselov S.V., Paulsen I.T., Pazour G.J.,
RA Purton S., Ral J.P., Riano-Pachon D.M., Riekhof W., Rymarquis L.,
RA Schroda M., Stern D., Umen J., Willows R., Wilson N., Zimmer S.L.,
RA Allmer J., Balk J., Bisova K., Chen C.J., Elias M., Gendler K., Hauser C.,
RA Lamb M.R., Ledford H., Long J.C., Minagawa J., Page M.D., Pan J.,
RA Pootakham W., Roje S., Rose A., Stahlberg E., Terauchi A.M., Yang P.,
RA Ball S., Bowler C., Dieckmann C.L., Gladyshev V.N., Green P., Jorgensen R.,
RA Mayfield S., Mueller-Roeber B., Rajamani S., Sayre R.T., Brokstein P.,
RA Dubchak I., Goodstein D., Hornick L., Huang Y.W., Jhaveri J., Luo Y.,
RA Martinez D., Ngau W.C., Otillar B., Poliakov A., Porter A., Szajkowski L.,
RA Werner G., Zhou K., Grigoriev I.V., Rokhsar D.S., Grossman A.R.;
RT "The Chlamydomonas genome reveals the evolution of key animal and plant
RT functions.";
RL Science 318:245-250(2007).
CC -!- FUNCTION: As part of the central apparatus of the cilium axoneme may
CC play a role in cilium movement and thereby cell motility.
CC {ECO:0000269|PubMed:20421426}.
CC -!- SUBUNIT: Part of the PDCP1 complex composed of CFAP46, CFAP54, CFAP74
CC and CFAP221; the PDCP1 complex binds calmodulin.
CC {ECO:0000269|PubMed:20421426}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, cilium axoneme
CC {ECO:0000269|PubMed:20421426}.
CC -!- SIMILARITY: Belongs to the CFAP74 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=EDP08468.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; GU564504; ADD85930.1; -; mRNA.
DR EMBL; DS496110; EDP08468.1; ALT_SEQ; Genomic_DNA.
DR PDB; 7SQC; EM; 3.80 A; O0/O1/O2=1-1940.
DR PDBsum; 7SQC; -.
DR AlphaFoldDB; D4P3R7; -.
DR PaxDb; D4P3R7; -.
DR EnsemblPlants; PNW82036; PNW82036; CHLRE_06g271150v5.
DR Gramene; PNW82036; PNW82036; CHLRE_06g271150v5.
DR eggNOG; ENOG502QPUP; Eukaryota.
DR OMA; RYKQLAT; -.
DR OrthoDB; 1032303at2759; -.
DR GO; GO:0005930; C:axoneme; IDA:UniProtKB.
DR GO; GO:0035082; P:axoneme assembly; IMP:UniProtKB.
DR GO; GO:0060294; P:cilium movement involved in cell motility; IMP:UniProtKB.
DR Gene3D; 2.60.40.10; -; 7.
DR InterPro; IPR013783; Ig-like_fold.
PE 1: Evidence at protein level;
KW 3D-structure; Cell projection; Cilium; Coiled coil; Cytoplasm;
KW Cytoskeleton.
FT CHAIN 1..1940
FT /note="Cilia- and flagella-associated protein 74"
FT /id="PRO_0000431702"
FT REGION 52..90
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 512..548
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1159..1336
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1373..1418
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1714..1737
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1894..1940
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 100..159
FT /evidence="ECO:0000255"
FT COMPBIAS 531..548
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1287..1309
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1373..1392
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1897..1912
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1940 AA; 203808 MW; DA05C23893584BAC CRC64;
MALTAEEAYL EELWREEDEQ SEEATAMQQM LAHQHEQQHD ALPSYQELMG QGSPAVTLRR
AKAAAAANGT SSPGIRGSPS PARGPGGRLP SLAMGPTVGA NVEQLKRRLQ TVVAEVEGHQ
QRYDKVLLEA NKATDLVHSM EAEIESLYVE AEELARRVPP AAERQRQVAT WLAQRSAALE
AQRQVLGGKQ LELIEAEAAL RAAETELDFM AEARNEIAAA QAAETAALSA AAATLRGKED
KAVAGYRRKQ AAEAARLADV AAAAEDLAQR RIQAAEEAQA QAMERLKANT ERIREGIASG
RDLLAAEQSR RREAVLGLKE SLAAVQADVA QQAELARVLQ RQRRDLQEKE FGALVEAGMN
PYEVFRRRDQ EAAAARQHEA ITSNIATRQL EVAAQLAREQ EAYAQKLAAR KREKKTREIF
DREMGPAAQQ ARTEAYMQAH TVGGVSMLDP TGRVQPFPSE AVVVKTRRFG RGGAPEEVLQ
QQLQRLGDDT QPKQLLLPAK YRNTADTDAA AVAGLGRGGG SDDEDGMGSP PRSPSLTQQL
AATQQSLTAT ASLAQQQQQA AGDRYKQLAT RALTKHEQGL MEAAKTRHKG GIGAPQTQMG
RTFGGDAFLP SPAVVEFKDF DVGRTVSASV QIINRSYKKN TFRVTGIPVE YCDVLDFAYK
LPGYLAPGVS GEVVISFTPK APVDIDTSLQ LLADTGPFEV PIRCRTKRAA LSVSPSAVDF
GAGVTLGECA TRTFTIANDG ALEVEFRLDS PQLSEDERAL LKSTANMAHL MTSATVAAAA
AAAAATASGQ GPRSARSFAN MGVADGAAPE TDTGPLVAPR EERRLAHGGF TVFPCVGHLK
GYSKVTFTVT FAPVVAAPAK LLLHASYKAP AMKRLALPHH EVTLTGTGRD VPVYVERPLI
DFQCCMLGHL YRDVLVVRNG GKSAMKVMVV PRPELEGFFE FSPDFGFVQA GDSFPITIRF
KPTPALLHAC RKHVVEPEEE QILEIGMRLS VPDQSLPVPF TLRAQITNTD LLFQPPALDF
GDCVMGERTA VKLLVRNPGR LPQTFGFVGL PSGLHFTPND GFGYVLPGEA LERLVSFQPP
IAGPQTFSIT AKTLAGRSFT LPCRANGVAP PVVLSSNRVR MPATPIGDTR TVSVVLINKT
DQPQSYEFAV PADSDLTLSP HVGRVPPQSR LRVQIDYSPR PPVDGEQQGA LPPPSAPNTA
RGPRDGGGGG AMANGNGSGG YEHYNDDGEP EDGEGDADGH TDTDSFQSPS ARASPVQPLG
RGGRGGRGGA AGEDEDEDED AGELPVRGKS SSSSKASSGR RSSSPQLGGR QGLAMGRINE
VPDDDDADAE AEAAAEAEQR TALAALKRIQ SMGGGDPGWY RWREHAITCY IKPQSQNPTP
SQSQSGQAPA ASAPSDGASG AAAAAETAAS SGPAAAPPPQ EIHLQVATCA VLPDLVLLAP
PDLPYVPLHQ CHCLDFGPVP VGQRVVRRLE LANEGEEPLV LGAESMDSRE VFGLVNALRP
VRPGASFRVM VSFTPQARTE YLEVLVLKSA RTRLRLALKG AGIAPELQLG PEGVTATGLD
MGDVLVGEAA ARTLTVTNVC PFPLSFTMRL AGKASAADPN PTMRQAFSCH PAEGTLAQGE
SCEVAVSFTP FSQRPYFEDV LQVVVPNQQD QLLVPLRGRG WREAVFVAGP DYPEPQPDPF
LLHHLAKQAG VALPPSVSGG GAAAGGAAVA AAAAGDKPST PAPGIKPPAT PPGGASKGKA
AAAAAAAAAA AAAGASEPRE LTLNFPHAIY PGEVATASFD VGSLKSTASG SAPGEVGVAE
LPAEAREAGW AVTDPPTAGA GGGGKVPLAA GERKPITLSY SAPAAPHPGM VAAYGHAEYR
VLKLQLTLKG GLAALSGGPE GRKVVVAARC RLLPGSRPPG EPVPPGVTPA PEPVAASGPG
AGAAGVKKLV PPPSPPKKAV
