CFA74_MOUSE
ID CFA74_MOUSE Reviewed; 1578 AA.
AC Q3UY96; A2AD78; A4QMY6; F6VQ96; Q14DK1; Q8BI90;
DT 18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT 04-FEB-2015, sequence version 3.
DT 03-AUG-2022, entry version 104.
DE RecName: Full=Cilia- and flagella-associated protein 74 {ECO:0000305};
GN Name=Cfap74 {ECO:0000312|MGI:MGI:1917130};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Aorta, Olfactory bulb, and Vein;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP PARTIAL NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: As part of the central apparatus of the cilium axoneme may
CC play a role in cilium movement. {ECO:0000250|UniProtKB:D4P3R7}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, cilium axoneme
CC {ECO:0000250|UniProtKB:D4P3R7}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q3UY96-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q3UY96-2; Sequence=VSP_057371, VSP_057372;
CC -!- SIMILARITY: Belongs to the CFAP74 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAI13169.1; Type=Miscellaneous discrepancy; Note=Probable cloning artifact.; Evidence={ECO:0000305};
CC Sequence=AAI16276.1; Type=Miscellaneous discrepancy; Note=Probable cloning artifact.; Evidence={ECO:0000305};
CC Sequence=BAC30745.1; Type=Miscellaneous discrepancy; Note=Probable cloning artifact.; Evidence={ECO:0000305};
CC Sequence=BAE22316.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AK040922; BAC30745.1; ALT_SEQ; mRNA.
DR EMBL; AK134858; BAE22316.1; ALT_SEQ; mRNA.
DR EMBL; AL670227; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC113168; AAI13169.1; ALT_SEQ; mRNA.
DR EMBL; BC116275; AAI16276.1; ALT_SEQ; mRNA.
DR RefSeq; NP_808342.3; NM_177674.5. [Q3UY96-2]
DR RefSeq; XP_006539111.1; XM_006539048.2. [Q3UY96-1]
DR AlphaFoldDB; Q3UY96; -.
DR iPTMnet; Q3UY96; -.
DR PhosphoSitePlus; Q3UY96; -.
DR jPOST; Q3UY96; -.
DR MaxQB; Q3UY96; -.
DR PaxDb; Q3UY96; -.
DR PRIDE; Q3UY96; -.
DR ProteomicsDB; 281652; -. [Q3UY96-1]
DR ProteomicsDB; 281653; -. [Q3UY96-2]
DR Antibodypedia; 26630; 36 antibodies from 11 providers.
DR Ensembl; ENSMUST00000151083; ENSMUSP00000123626; ENSMUSG00000078490. [Q3UY96-1]
DR GeneID; 544678; -.
DR KEGG; mmu:544678; -.
DR UCSC; uc008wdk.2; mouse. [Q3UY96-1]
DR UCSC; uc008wdl.1; mouse. [Q3UY96-2]
DR CTD; 85452; -.
DR MGI; MGI:1917130; Cfap74.
DR VEuPathDB; HostDB:ENSMUSG00000078490; -.
DR GeneTree; ENSGT00900000141054; -.
DR HOGENOM; CLU_243984_0_0_1; -.
DR InParanoid; Q3UY96; -.
DR PhylomeDB; Q3UY96; -.
DR BioGRID-ORCS; 544678; 1 hit in 68 CRISPR screens.
DR ChiTaRS; Cfap74; mouse.
DR PRO; PR:Q3UY96; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; Q3UY96; protein.
DR Bgee; ENSMUSG00000078490; Expressed in spermatocyte and 56 other tissues.
DR ExpressionAtlas; Q3UY96; baseline and differential.
DR GO; GO:0005930; C:axoneme; ISS:UniProtKB.
DR GO; GO:0035082; P:axoneme assembly; ISS:UniProtKB.
DR Gene3D; 2.60.40.10; -; 4.
DR InterPro; IPR013783; Ig-like_fold.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell projection; Cilium; Coiled coil; Cytoplasm;
KW Cytoskeleton; Reference proteome.
FT CHAIN 1..1578
FT /note="Cilia- and flagella-associated protein 74"
FT /evidence="ECO:0000305"
FT /id="PRO_0000324665"
FT REGION 1..21
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 33..67
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 103..156
FT /evidence="ECO:0000255"
FT COILED 330..378
FT /evidence="ECO:0000255"
FT VAR_SEQ 783..784
FT /note="LY -> IY (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_057371"
FT VAR_SEQ 785..1578
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_057372"
SQ SEQUENCE 1578 AA; 178928 MW; E47D20FD5B3CB596 CRC64;
MEEPTVQFSD EDLVDNFPPM DDERVQLEDL EFEVERPSEG LEDEGSHSSA KKESKGAEKM
RKSTTKDQVQ AFHLRKSLNL LDKMHEEKDV FIQKTKGELH ICRQRMDLLN KQQESLAAEI
ATEKEANNMA AIGRLQAASR RLQTELENEK DLQSKITAML KDSENAMWHI EIQKGQFEDV
RKHHEAEAEA RQRGLEVHSA RQLQREREAM EKSEKNRLLR ARKSLHTQKE LGLRHQKLVE
DAQRNHRIAV KFLKASLGRV REREQKEEME SRTHMQRRMD AVLSLKNNIT ASRETLKKFQ
AWGQTRADLA KQKALTEKEV ILSQGGDAFK YLFHQRRHQE LEAQKRAFEE EQKLRKQEIV
NRILKEEAEE EQRKRRQHPL SKPINRRTLR DKTWQYISDF CEGKSVLTSQ QERERELLLY
PKTVCVIKAI SSESVQVDLG SISTEDEVLA EPDISGLWNK ESYQVPKEDM ERKPVGGSKM
EKDILARTME QLRSGVVQKQ VVSGREFKGR PFNSKPEVIH FKDFDIGKVY KKKITLINAT
YTINYCKLVG VEENLKDFIH IDFEPPGPMS AGMSCEVLVT FKPMINKDLE GNVSFLAQTG
SFSVPLKCST KKCSLSLDKE LIDFGTYVVG ETTSRIITLT NVGGLGTKFK FLPDSEFYEM
DESQPAMKIS SLFTCEEKII YEKIMTSLSE QQLEVNDSSL VDLQSLKESE KQLDDPEVTT
AAVSAMTMIP SEEQAEITLG EVTEGEIGPF SSVKVPITFT PVIPGEVQTK FKVMFKNPQS
PPLYFRATGT AIDVPVWVPK ATVDLKICMY DRLYQDSITV HTRSKAALRL KFEVCKELRG
HIELLPETGY IQAQSTYSVQ LKFLPRQSLP EDARKYFDPA SRVLEAPMTI RVADQIKPVR
FTVQAIVTTS DLEINPSEIN FGYCTIYEAI RTEICLSNLS LLPQEFGFVG LPKYVDIQPN
DGFGTILPLE TLHLDVIFQP IKAKEYKFEL VCKSEINRCF KVSCQAVGVH PPLELSHYQI
KFSATSLYDT SVSTLYVINS HLSMNKMIHS LPRIGSEEAA PVGPTSFEFL LPPNSPITIS
PSVGTVLPGK RCLIQVAFQP VLPKEIIYKE ASQILNKEIE TKPVSQKEIV QRKELWKQSF
SVVRVHNRDR PTRVSTPQAT ELQRPVINSS STEFQIAQAT LSKAFQGKFN RFVIPCVVAS
GDIKDRKTAE PLSFSPHNTL YLELWCPAVA PFIVVTSHKG KTDFNFGDIA VGHRSVKKIT
LQNICNEDLT LEYSVLNPNG PFVRLNPFNK LRSGETQTLV LSFSPHENIL AQETLDIITK
RGTLSLTLFG MGVASMITCS IDGNILNMGY VLARESVSTN FKLQNESSLP IKFWVRLESL
SRKKAEAHQQ LPKFITSHEQ RAEIVGTQNY NGQSVFSIVP VEGLMFPGKA QEFTVTFSPD
HESLFFSDLL KVVLFEKKVS HQILLKGAAR EHMMFVEGGD PLDVPVESLA VVTAFDTEHK
EEAEELKPIL VTLNYVQLDT DTTTSPATRE LQVGCIRTTQ PSPRRPDHPL MVSTLLQLRG
DVKETYKVTF VAHVVTGL
