CFA91_MOUSE
ID CFA91_MOUSE Reviewed; 783 AA.
AC Q8BRC6; E9PZ32; E9QND1;
DT 19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT 06-MAR-2013, sequence version 3.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Cilia- and flagella-associated protein 91 {ECO:0000250|UniProtKB:Q7Z4T9};
DE Short=CFAP91 {ECO:0000305};
DE AltName: Full=AMY-1-associating protein expressed in testis 1 homolog;
DE Short=AAT-1;
DE AltName: Full=MYCBP/AMY-1-associated testis-expressed protein 1;
DE AltName: Full=Protein MAATS1 {ECO:0000305};
GN Name=Cfap91 {ECO:0000312|MGI:MGI:2443598}; Synonyms=Aat1, Maats1, Spata26;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=12223483; DOI=10.1074/jbc.m206201200;
RA Yukitake H., Furusawa M., Taira T., Iguchi-Ariga S.M.M., Ariga H.;
RT "AAT-1, a novel testis-specific AMY-1-binding protein, forms a quaternary
RT complex between AMY-1, A-kinase anchor protein 84 and a regulatory subunit
RT of cAMP-dependent protein kinase and is phosphorylated by its kinase.";
RL J. Biol. Chem. 277:45480-45492(2002).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Involved in sperm flagellum axonemal organization and
CC function (By similarity). May regulate cilium motility through its role
CC in the assembly of the axonemal radial spokes (By similarity).
CC {ECO:0000250|UniProtKB:A8IH47, ECO:0000250|UniProtKB:Q7Z4T9}.
CC -!- SUBUNIT: Part of a complex containing MYCBP, AKAP1 and PRKAR2B.
CC {ECO:0000250|UniProtKB:Q7Z4T9}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q7Z4T9}.
CC Mitochondrion {ECO:0000250|UniProtKB:Q7Z4T9}. Cytoplasm, cytoskeleton,
CC cilium axoneme {ECO:0000250|UniProtKB:A8IH47}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8BRC6-2; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8BRC6-1; Sequence=VSP_045751, VSP_045752;
CC -!- TISSUE SPECIFICITY: Expressed in the testis, in cells involved in
CC spermatogenesis. {ECO:0000269|PubMed:12223483}.
CC -!- DEVELOPMENTAL STAGE: Expression begins in 1 week old mice.
CC {ECO:0000269|PubMed:12223483}.
CC -!- PTM: Phosphorylated by PKA. {ECO:0000250|UniProtKB:Q7Z4T9}.
CC -!- SIMILARITY: Belongs to the CFAP91 family.
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DR EMBL; AK045121; BAC32231.1; -; mRNA.
DR EMBL; AC154809; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS37341.1; -. [Q8BRC6-2]
DR RefSeq; NP_001074494.1; NM_001081025.1. [Q8BRC6-2]
DR AlphaFoldDB; Q8BRC6; -.
DR SMR; Q8BRC6; -.
DR BioGRID; 235850; 3.
DR STRING; 10090.ENSMUSP00000023501; -.
DR iPTMnet; Q8BRC6; -.
DR PhosphoSitePlus; Q8BRC6; -.
DR MaxQB; Q8BRC6; -.
DR PaxDb; Q8BRC6; -.
DR PRIDE; Q8BRC6; -.
DR ProteomicsDB; 280087; -. [Q8BRC6-2]
DR ProteomicsDB; 281112; -. [Q8BRC6-1]
DR Ensembl; ENSMUST00000023501; ENSMUSP00000023501; ENSMUSG00000022805. [Q8BRC6-2]
DR Ensembl; ENSMUST00000114740; ENSMUSP00000110388; ENSMUSG00000022805. [Q8BRC6-1]
DR GeneID; 320214; -.
DR KEGG; mmu:320214; -.
DR UCSC; uc007zer.1; mouse. [Q8BRC6-2]
DR UCSC; uc007zes.1; mouse. [Q8BRC6-1]
DR CTD; 89876; -.
DR MGI; MGI:2443598; Cfap91.
DR VEuPathDB; HostDB:ENSMUSG00000022805; -.
DR eggNOG; ENOG502QRFI; Eukaryota.
DR GeneTree; ENSGT00390000003024; -.
DR HOGENOM; CLU_011633_0_0_1; -.
DR InParanoid; Q8BRC6; -.
DR OMA; HLNAQWS; -.
DR OrthoDB; 496618at2759; -.
DR PhylomeDB; Q8BRC6; -.
DR TreeFam; TF328648; -.
DR BioGRID-ORCS; 320214; 1 hit in 72 CRISPR screens.
DR PRO; PR:Q8BRC6; -.
DR Proteomes; UP000000589; Chromosome 16.
DR RNAct; Q8BRC6; protein.
DR Bgee; ENSMUSG00000022805; Expressed in ventricular system choroidal fissure and 64 other tissues.
DR Genevisible; Q8BRC6; MM.
DR GO; GO:0005930; C:axoneme; ISS:UniProtKB.
DR GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR GO; GO:0031514; C:motile cilium; ISS:UniProtKB.
DR GO; GO:1904158; P:axonemal central apparatus assembly; ISS:UniProtKB.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0003341; P:cilium movement; ISS:UniProtKB.
DR GO; GO:0007283; P:spermatogenesis; ISS:UniProtKB.
DR InterPro; IPR026720; CFAP91.
DR InterPro; IPR032840; CFAP91_dom.
DR PANTHER; PTHR22455; PTHR22455; 1.
DR Pfam; PF14738; CFAP91; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell projection; Cytoplasm; Cytoskeleton;
KW Differentiation; Mitochondrion; Reference proteome; Spermatogenesis.
FT CHAIN 1..783
FT /note="Cilia- and flagella-associated protein 91"
FT /id="PRO_0000064419"
FT REGION 748..783
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 762..776
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 339..345
FT /note="DIRKLMG -> EAEEMAQ (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_045751"
FT VAR_SEQ 346..783
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_045752"
FT CONFLICT 238
FT /note="E -> K (in Ref. 1; BAC32231)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 783 AA; 91623 MW; 12237970B7041685 CRC64;
MSQTVTIQEP RPNDQRIPYQ CREVRRAKGG FANRTYDYLY DPLFIVSSER DHAQANIQAT
LIRSRLKKVP NFRSMFSNLF HHPRYSMYWS KTDPVPLHVT REWRGQEAKH KEVLRLQAAM
DTSFQMPKEK DEDPDVSGKN RYKFFDRPFL PFLQQMPLNV VLSPVKTQPL LLTPESSKYA
IIPTKSTVAT QTDYRDADVQ TDPYSPEYVV CQDTIPELLT LANLTWGRGL PAGQAEVEII
ERAREKRAWE ATLPPLNDSV QAEKRRKMMN AMERKEWAFR EGEIEKLQEL RLEVLKQLLK
RREEDQNELN MRHLNDQWYK LQEAKEAKVA QIRHKHVSDI RKLMGKGKNI EGKLQRRDII
SDYSNFASQV YGPLSRLGRF PDNNSEDFVV RNHYLNTYEG LVELESSLPD FVTQPRIKPP
KPQIITTKAG FLKRTARMDY ELAEVHKALV DKKNKGLEGT KSLRFLQKNP ISQARLPTPS
LEMTSYEEGE IEMAVIYLQK LLRGRVVQNM MFEGKEKRLE LILELRTSHA LQEDDKLVKK
AEKQVTLALQ RQRNLHEDKL SVIENHLGDL EGRVLADMFD FLSKELVRLQ EERRIHAFAM
LAERQRRMRE AEESGRRQVE QKRLQQEDMI FKEVIKVHQS TVTSYLEDII LNTEERTAEE
QARKEIEKIA EEINNIAYEM ENRRTYLQSE EIVAELVYSF LIPEVQKDFV KEKVRNAQRK
HILAAHEIIH SNTETMLEEQ VYKELQSEDF ELEEEAESLD SEVPTVSVSK TSTIKPTQDE
GEG
