CFA91_TRYB2
ID CFA91_TRYB2 Reviewed; 709 AA.
AC Q386N1;
DT 23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT 22-NOV-2005, sequence version 1.
DT 03-AUG-2022, entry version 66.
DE RecName: Full=Cilia- and flagella-associated protein 91;
DE AltName: Full=Flagellar-associated protein 91;
GN Name=CFAP91; ORFNames=Tb11.02.0354;
OS Trypanosoma brucei brucei (strain 927/4 GUTat10.1).
OC Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina;
OC Trypanosomatida; Trypanosomatidae; Trypanosoma.
OX NCBI_TaxID=185431;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=927/4 GUTat10.1;
RX PubMed=16020726; DOI=10.1126/science.1112642;
RA Berriman M., Ghedin E., Hertz-Fowler C., Blandin G., Renauld H.,
RA Bartholomeu D.C., Lennard N.J., Caler E., Hamlin N.E., Haas B., Bohme U.,
RA Hannick L., Aslett M.A., Shallom J., Marcello L., Hou L., Wickstead B.,
RA Alsmark U.C.M., Arrowsmith C., Atkin R.J., Barron A.J., Bringaud F.,
RA Brooks K., Carrington M., Cherevach I., Chillingworth T.J., Churcher C.,
RA Clark L.N., Corton C.H., Cronin A., Davies R.M., Doggett J., Djikeng A.,
RA Feldblyum T., Field M.C., Fraser A., Goodhead I., Hance Z., Harper D.,
RA Harris B.R., Hauser H., Hostetler J., Ivens A., Jagels K., Johnson D.,
RA Johnson J., Jones K., Kerhornou A.X., Koo H., Larke N., Landfear S.,
RA Larkin C., Leech V., Line A., Lord A., Macleod A., Mooney P.J., Moule S.,
RA Martin D.M., Morgan G.W., Mungall K., Norbertczak H., Ormond D., Pai G.,
RA Peacock C.S., Peterson J., Quail M.A., Rabbinowitsch E., Rajandream M.A.,
RA Reitter C., Salzberg S.L., Sanders M., Schobel S., Sharp S., Simmonds M.,
RA Simpson A.J., Tallon L., Turner C.M., Tait A., Tivey A.R., Van Aken S.,
RA Walker D., Wanless D., Wang S., White B., White O., Whitehead S.,
RA Woodward J., Wortman J., Adams M.D., Embley T.M., Gull K., Ullu E.,
RA Barry J.D., Fairlamb A.H., Opperdoes F., Barrell B.G., Donelson J.E.,
RA Hall N., Fraser C.M., Melville S.E., El-Sayed N.M.A.;
RT "The genome of the African trypanosome Trypanosoma brucei.";
RL Science 309:416-422(2005).
RN [2]
RP FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, AND SUBUNIT.
RX PubMed=32161152; DOI=10.1136/jmedgenet-2019-106775;
RA Martinez G., Beurois J., Dacheux D., Cazin C., Bidart M., Kherraf Z.E.,
RA Robinson D.R., Satre V., Le Gac G., Ka C., Gourlaouen I., Fichou Y.,
RA Petre G., Dulioust E., Zouari R., Thierry-Mieg N., Toure A., Arnoult C.,
RA Bonhivers M., Ray P., Coutton C.;
RT "Biallelic variants in MAATS1 encoding CFAP91, a calmodulin-associated and
RT spoke-associated complex protein, cause severe astheno-teratozoospermia and
RT male infertility.";
RL J. Med. Genet. 57:708-716(2020).
CC -!- FUNCTION: Axoneme-associated protein involved in flagellum motility and
CC central pair positioning. {ECO:0000269|PubMed:32161152}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, flagellum axoneme
CC {ECO:0000269|PubMed:32161152}.
CC -!- DISRUPTION PHENOTYPE: Knockdown of CFAP91 does not affect the cell
CC morphology and the flagella appears normal in number and length.
CC However, flagella exhibit an abnormal axoneme with a rotated central
CC pair complex (CPC) and an impaired flagellar motility.
CC {ECO:0000269|PubMed:32161152}.
CC -!- SIMILARITY: Belongs to the CFAP91 family.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CH464491; EAN79250.1; -; Genomic_DNA.
DR RefSeq; XP_828362.1; XM_823269.1.
DR AlphaFoldDB; Q386N1; -.
DR STRING; 5691.EAN79250; -.
DR PaxDb; Q386N1; -.
DR GeneID; 3664170; -.
DR KEGG; tbr:Tb11.02.0354; -.
DR VEuPathDB; TriTrypDB:Tb927.11.2790; -.
DR eggNOG; ENOG502QRFI; Eukaryota.
DR InParanoid; Q386N1; -.
DR Proteomes; UP000008524; Chromosome 11 Scaffold 1.
DR GO; GO:0005930; C:axoneme; IDA:UniProtKB.
DR GO; GO:0005929; C:cilium; IDA:GeneDB.
DR GO; GO:0005737; C:cytoplasm; IDA:GeneDB.
DR GO; GO:0031514; C:motile cilium; IEA:UniProtKB-KW.
DR GO; GO:1904158; P:axonemal central apparatus assembly; IMP:UniProtKB.
DR GO; GO:0060285; P:cilium-dependent cell motility; IMP:UniProtKB.
DR GO; GO:0003356; P:regulation of cilium beat frequency; IDA:UniProtKB.
DR InterPro; IPR026720; CFAP91.
DR InterPro; IPR032840; CFAP91_dom.
DR PANTHER; PTHR22455; PTHR22455; 1.
DR Pfam; PF14738; CFAP91; 1.
PE 1: Evidence at protein level;
KW Cell projection; Cilium; Coiled coil; Cytoplasm; Cytoskeleton; Flagellum;
KW Reference proteome.
FT CHAIN 1..709
FT /note="Cilia- and flagella-associated protein 91"
FT /id="PRO_0000454111"
FT REGION 94..116
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 333..360
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 690..709
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 334..348
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 709 AA; 80034 MW; F4AC595B155CBCC7 CRC64;
MYYRTQQKPA SVLGANGFTA EVGGRDRVRY FRPPVEVAGV PMRYSGIDTV RFSATAPTTP
NLPRTFAETR FDRPTSMAAG LVGPPHHLPS LPRRAGAAPP HPVKAKSVKL PPQRTKRVEK
LASTIGTDGK CDAAMQTVFR ENEAQTLPYT PNYYIPEGGD PNPQVLGLME LHWNEGLPAG
KEEVELIQRL RRRRAVEASL LDETSKEAKM ENFCKLYDLE VKEREEREQH FENLRKRRLD
QIHEALQERE RARDELNRQR LEKVKEQRLA NLQRVIEQME SKRVGMGRKA LAEQASKAPA
AAGRGLYAVD PIETFKRKPD LIQTYARRGT GAVEPQLCTN NGGGSASASP RRDRSRKPLR
NYRQYDIQPA MLKYEGGIAE LEANKIPKIQ QIAPNAFAAP ENHAINSLPS LYQRREATRV
VEALEYVHAK IHKSDTPAET IRVLELYRAT PRPQRPDTPT LELEGDVNEA VEESCTLLQR
LLRGRAVQND FFDGKERCRG LIEELQAASN AKYAERSAEE KQAEAEEKMR EAIADSIGNE
AQGDIICDTL DYLFHEMTRQ QDLLALEALR HEAEAVRAER EAREAELRAQ ERILHDKEAV
QYAAYVRAIE DIVECYSHDL YATVAEECAM EEAIEAECQR LEQLPPPTSN ILSDPEVAEN
LVCDVLDNFV LPAVIDMVRM KPEELDRKAP AAAAAGFRQR SPNEKETKE
