ID   CFA95_BOVIN             Reviewed;         232 AA.
AC   Q32L77;
DT   09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT   06-DEC-2005, sequence version 1.
DT   03-AUG-2022, entry version 73.
DE   RecName: Full=Cilia- and flagella-associated protein 95 {ECO:0000250|UniProtKB:Q5VTT2};
GN   Name=CFAP95 {ECO:0000250|UniProtKB:Q5VTT2};
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Crossbred X Angus; TISSUE=Liver;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0007744|PDB:7RRO}
RP   STRUCTURE BY ELECTRON MICROSCOPY (3.40 ANGSTROMS), FUNCTION, SUBCELLULAR
RP   LOCATION, AND TISSUE SPECIFICITY.
RX   PubMed=34715025; DOI=10.1016/j.cell.2021.10.007;
RA   Gui M., Farley H., Anujan P., Anderson J.R., Maxwell D.W., Whitchurch J.B.,
RA   Botsch J.J., Qiu T., Meleppattu S., Singh S.K., Zhang Q., Thompson J.,
RA   Lucas J.S., Bingle C.D., Norris D.P., Roy S., Brown A.;
RT   "De novo identification of mammalian ciliary motility proteins using cryo-
RT   EM.";
RL   Cell 184:5791-5806.e19(2021).
CC   -!- FUNCTION: Microtubule inner protein (MIP) part of the dynein-decorated
CC       doublet microtubules (DMTs) in cilia axoneme, which is required for
CC       motile cilia beating. {ECO:0000269|PubMed:34715025}.
CC   -!- SUBUNIT: Interacts with MYH9. Interacts with MYH10.
CC       {ECO:0000250|UniProtKB:Q5VTT2}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, cilium axoneme
CC       {ECO:0000269|PubMed:34715025}. Cell membrane
CC       {ECO:0000250|UniProtKB:Q5VTT2}; Single-pass membrane protein
CC       {ECO:0000255}. Cytoplasm {ECO:0000250|UniProtKB:Q5VTT2}.
CC   -!- TISSUE SPECIFICITY: Expressed in trachea multiciliated cells.
CC       {ECO:0000269|PubMed:34715025}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; BC109724; AAI09725.1; -; mRNA.
DR   RefSeq; NP_001073093.1; NM_001079625.2.
DR   PDB; 7RRO; EM; 3.40 A; 0/7=1-232.
DR   PDBsum; 7RRO; -.
DR   AlphaFoldDB; Q32L77; -.
DR   STRING; 9913.ENSBTAP00000044717; -.
DR   PaxDb; Q32L77; -.
DR   Ensembl; ENSBTAT00000047514; ENSBTAP00000044717; ENSBTAG00000033396.
DR   GeneID; 616736; -.
DR   KEGG; bta:616736; -.
DR   CTD; 138255; -.
DR   VEuPathDB; HostDB:ENSBTAG00000033396; -.
DR   VGNC; VGNC:52738; CFAP95.
DR   eggNOG; ENOG502R194; Eukaryota.
DR   GeneTree; ENSGT00390000014593; -.
DR   HOGENOM; CLU_108076_0_0_1; -.
DR   InParanoid; Q32L77; -.
DR   OMA; WHQAREA; -.
DR   OrthoDB; 1568741at2759; -.
DR   TreeFam; TF328471; -.
DR   Proteomes; UP000009136; Chromosome 8.
DR   Bgee; ENSBTAG00000033396; Expressed in spermatid and 50 other tissues.
DR   GO; GO:0005879; C:axonemal microtubule; IDA:UniProtKB.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; ISS:UniProtKB.
DR   InterPro; IPR027905; CFAP95.
DR   PANTHER; PTHR35069; PTHR35069; 1.
DR   Pfam; PF15139; DUF4572; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cell membrane; Cell projection; Cytoplasm; Cytoskeleton;
KW   Glycoprotein; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..232
FT                   /note="Cilia- and flagella-associated protein 95"
FT                   /id="PRO_0000271062"
FT   TOPO_DOM        1..96
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        97..115
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        116..232
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        75
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   232 AA;  26623 MW;  E3B12EBA010426F7 CRC64;
     MDSSDSSCQE WCSHGQYWLE LGPTDLMERK GSLTLRSHHK KYSKPVLVYS WHRDRETYPK
     DYDIEGPEEV KKLCNSTYRR LGTSEPPVWI SETREKMAQV CLNTKLAKIK SKALLNEETM
     NSGIIERDTG LPATGFGALF TRHSPDWSKM CTLTTYAEEY APPYEYQPLG DPCQDDDYSI
     VHRKCRSQFT DLDGSKRVGI NTWHDESGIY ANSYVKRKLY SLTGGPIAPF LK