CFA95_MOUSE
ID CFA95_MOUSE Reviewed; 228 AA.
AC Q9CQC3;
DT 09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 102.
DE RecName: Full=Cilia- and flagella-associated protein 95 {ECO:0000250|UniProtKB:Q5VTT2};
GN Name=Cfap95 {ECO:0000250|UniProtKB:Q5VTT2};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP TISSUE SPECIFICITY.
RX PubMed=28345668; DOI=10.1038/srep45311;
RA Zhou S., Liu Y., Ma Y., Zhang X., Li Y., Wen J.;
RT "C9ORF135 encodes a membrane protein whose expression is related to
RT pluripotency in human embryonic stem cells.";
RL Sci. Rep. 7:45311-45311(2017).
CC -!- FUNCTION: Microtubule inner protein (MIP) part of the dynein-decorated
CC doublet microtubules (DMTs) in cilia axoneme, which is required for
CC motile cilia beating. {ECO:0000250|UniProtKB:Q32L77}.
CC -!- SUBUNIT: Interacts with MYH9. Interacts with MYH10.
CC {ECO:0000250|UniProtKB:Q5VTT2}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, cilium axoneme
CC {ECO:0000250|UniProtKB:Q32L77}. Cell membrane
CC {ECO:0000250|UniProtKB:Q5VTT2}; Single-pass membrane protein
CC {ECO:0000255}. Cytoplasm {ECO:0000250|UniProtKB:Q5VTT2}.
CC -!- TISSUE SPECIFICITY: Highly expressed in the testis. Weakly or not
CC expressed in embryonic stem cells. {ECO:0000269|PubMed:28345668}.
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DR EMBL; AK006473; BAB24606.1; -; mRNA.
DR EMBL; AK014972; BAB29647.1; -; mRNA.
DR EMBL; BC049563; AAH49563.1; -; mRNA.
DR CCDS; CCDS29708.1; -.
DR RefSeq; NP_080464.1; NM_026188.2.
DR AlphaFoldDB; Q9CQC3; -.
DR SMR; Q9CQC3; -.
DR STRING; 10090.ENSMUSP00000048680; -.
DR PhosphoSitePlus; Q9CQC3; -.
DR PaxDb; Q9CQC3; -.
DR PRIDE; Q9CQC3; -.
DR Antibodypedia; 12367; 26 antibodies from 10 providers.
DR DNASU; 67483; -.
DR Ensembl; ENSMUST00000035849; ENSMUSP00000048680; ENSMUSG00000033053.
DR GeneID; 67483; -.
DR KEGG; mmu:67483; -.
DR UCSC; uc008had.1; mouse.
DR CTD; 138255; -.
DR MGI; MGI:1914733; 1700028P14Rik.
DR VEuPathDB; HostDB:ENSMUSG00000033053; -.
DR eggNOG; ENOG502R194; Eukaryota.
DR GeneTree; ENSGT00390000014593; -.
DR HOGENOM; CLU_108076_0_0_1; -.
DR InParanoid; Q9CQC3; -.
DR OMA; WHQAREA; -.
DR OrthoDB; 1568741at2759; -.
DR PhylomeDB; Q9CQC3; -.
DR TreeFam; TF328471; -.
DR BioGRID-ORCS; 67483; 2 hits in 73 CRISPR screens.
DR PRO; PR:Q9CQC3; -.
DR Proteomes; UP000000589; Chromosome 19.
DR RNAct; Q9CQC3; protein.
DR Bgee; ENSMUSG00000033053; Expressed in seminiferous tubule of testis and 62 other tissues.
DR Genevisible; Q9CQC3; MM.
DR GO; GO:0005879; C:axonemal microtubule; ISS:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; ISS:UniProtKB.
DR InterPro; IPR027905; CFAP95.
DR PANTHER; PTHR35069; PTHR35069; 1.
DR Pfam; PF15139; DUF4572; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Cell projection; Cytoplasm; Cytoskeleton; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..228
FT /note="Cilia- and flagella-associated protein 95"
FT /id="PRO_0000271064"
FT TOPO_DOM 1..122
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 123..139
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 140..228
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 123..147
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 228 AA; 26594 MW; 9F7D91518539D125 CRC64;
MGTFDSQCHE CFESKERWYE IGPTDLLERK GSLTLRSHHK KYSEPVLVYS WHRNREAFPK
DYDIESLETV KNLCMSTYKR FGSDSPRWMS EAREQMAQVL VNKDLVEKKK TGLLDEETLC
PGILERDPEL PPTGFRDVPM ESPPDPRKPC FSTTYSEDFT PQYEYPPPAC PRREEYSIVH
RKCRSQFTDL NGSKRLGINT WHDESGIYAN SEAKQKLYAL ARNPIVPF
