CFA97_MOUSE
ID CFA97_MOUSE Reviewed; 541 AA.
AC Q6ZPR1; Q9D462;
DT 13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT 13-NOV-2007, sequence version 2.
DT 03-AUG-2022, entry version 80.
DE RecName: Full=Cilia- and flagella-associated protein 97 {ECO:0000312|MGI:MGI:1914006};
GN Name=Cfap97 {ECO:0000312|MGI:MGI:1914006};
GN Synonyms=Kiaa1430 {ECO:0000312|MGI:MGI:1914006};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Embryonic tail;
RX PubMed=14621295; DOI=10.1093/dnares/10.4.167;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S.,
RA Saga Y., Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: III.
RT The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL DNA Res. 10:167-180(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-160 AND SER-161, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=17242355; DOI=10.1073/pnas.0609836104;
RA Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
RT "Large-scale phosphorylation analysis of mouse liver.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-8; SER-19; SER-160 AND
RP SER-161, AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Kidney, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [6]
RP TISSUE SPECIFICITY.
RX PubMed=32785227; DOI=10.1371/journal.pgen.1008954;
RA Oura S., Kazi S., Savolainen A., Nozawa K., Castaneda J., Yu Z., Miyata H.,
RA Matzuk R.M., Hansen J.N., Wachten D., Matzuk M.M.,
RA Prunskaite-Hyyrylaeinen R.;
RT "Cfap97d1 is important for flagellar axoneme maintenance and male mouse
RT fertility.";
RL PLoS Genet. 16:e1008954-e1008954(2020).
CC -!- TISSUE SPECIFICITY: Highly expressed in testis with lower levels
CC detected in other tissues including lung, heart and kidney.
CC {ECO:0000269|PubMed:32785227}.
CC -!- SIMILARITY: Belongs to the CFAP97 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC98168.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AK129358; BAC98168.1; ALT_INIT; mRNA.
DR EMBL; AK016776; BAB30422.1; -; mRNA.
DR EMBL; BC058092; AAH58092.1; -; mRNA.
DR CCDS; CCDS22288.1; -.
DR RefSeq; NP_080023.1; NM_025747.3.
DR RefSeq; XP_006509539.2; XM_006509476.3.
DR RefSeq; XP_006509540.1; XM_006509477.3.
DR RefSeq; XP_006509541.1; XM_006509478.3.
DR RefSeq; XP_006509542.1; XM_006509479.2.
DR RefSeq; XP_006509543.1; XM_006509480.2.
DR RefSeq; XP_006509544.1; XM_006509481.2.
DR RefSeq; XP_006509545.1; XM_006509482.3.
DR AlphaFoldDB; Q6ZPR1; -.
DR SMR; Q6ZPR1; -.
DR STRING; 10090.ENSMUSP00000106005; -.
DR iPTMnet; Q6ZPR1; -.
DR PhosphoSitePlus; Q6ZPR1; -.
DR MaxQB; Q6ZPR1; -.
DR PaxDb; Q6ZPR1; -.
DR PRIDE; Q6ZPR1; -.
DR ProteomicsDB; 281394; -.
DR Antibodypedia; 56002; 34 antibodies from 13 providers.
DR Ensembl; ENSMUST00000034048; ENSMUSP00000034048; ENSMUSG00000031631.
DR Ensembl; ENSMUST00000164504; ENSMUSP00000133211; ENSMUSG00000031631.
DR GeneID; 66756; -.
DR KEGG; mmu:66756; -.
DR UCSC; uc009lpv.1; mouse.
DR CTD; 57587; -.
DR MGI; MGI:1914006; Cfap97.
DR VEuPathDB; HostDB:ENSMUSG00000031631; -.
DR eggNOG; ENOG502S0ZF; Eukaryota.
DR GeneTree; ENSGT00390000010356; -.
DR HOGENOM; CLU_040301_1_0_1; -.
DR InParanoid; Q6ZPR1; -.
DR OMA; KRNACRN; -.
DR OrthoDB; 947267at2759; -.
DR PhylomeDB; Q6ZPR1; -.
DR BioGRID-ORCS; 66756; 4 hits in 73 CRISPR screens.
DR ChiTaRS; Cfap97; mouse.
DR PRO; PR:Q6ZPR1; -.
DR Proteomes; UP000000589; Chromosome 8.
DR RNAct; Q6ZPR1; protein.
DR Bgee; ENSMUSG00000031631; Expressed in spermatocyte and 221 other tissues.
DR ExpressionAtlas; Q6ZPR1; baseline and differential.
DR Genevisible; Q6ZPR1; MM.
DR GO; GO:0007283; P:spermatogenesis; IBA:GO_Central.
DR InterPro; IPR038791; Cfap97/Hemingway.
DR InterPro; IPR029488; Hmw/CFAP97.
DR PANTHER; PTHR23035; PTHR23035; 1.
DR Pfam; PF13879; Hmw_CFAP97; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Phosphoprotein; Reference proteome.
FT CHAIN 1..541
FT /note="Cilia- and flagella-associated protein 97"
FT /evidence="ECO:0000305"
FT /id="PRO_0000309225"
FT REGION 28..47
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 93..296
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 313..337
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 406..430
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 495..514
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 382..459
FT /evidence="ECO:0000255"
FT COMPBIAS 109..125
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 144..170
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 171..204
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 218..251
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 263..281
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 313..327
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 498..514
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 8
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 19
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 155
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q9P2B7"
FT MOD_RES 160
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17242355,
FT ECO:0007744|PubMed:21183079"
FT MOD_RES 161
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17242355,
FT ECO:0007744|PubMed:21183079"
FT MOD_RES 230
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9P2B7"
FT MOD_RES 258
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q66H34"
SQ SEQUENCE 541 AA; 60837 MW; EEC54FB097C957EA CRC64;
MDRFGDISEG EVDHSFFDSD FEDAKKCESN SIFDKQNDDD LKEGINKDTK NVNLKFGVQN
DHLKEKIDNN TENVNLKLGL QTTENYLTQK GNERKANFSS KEQHIENDPT QARSSSVLTS
SRSKKSCDAT KGHKLNLPVP DRIPKIVKGE DDYYTDGEES SDDGKKYVRS KSAKPSSNLK
KNVSKKYSSS SLSSSSSRSN SDCSDMGSDR QRRSESHSSG KCVSSVTPSS PKQRCKSGRK
SSAQPSSTKQ KTGDYHESEG NVPDITPLST PDVSPAQSLE LGQPPDQKVK VKKQENVSRD
VYEDAEALKN DSRCVKSAKR KEKHGQSFAP KSSVLDANLD RRSKQKVLHD TMDLNHLLKA
FLQLDKKGPQ KHHFEQPAII PRKNYSFTRE EVRQIDRENQ RLLKELSRQA EKPGSKSTIP
GRSIGHPPKL YHSALNRQRE QQRIERENMA LLKRLEAVKP TVGMKRSEQL MDYHRNMSYL
NPSPSVRRVR STLGHYSPLR GASRTSSATS GLSCKTDRSV LDTSNGFLLR PKPPNVRTAW
L
