CFD1_SCHJY
ID CFD1_SCHJY Reviewed; 612 AA.
AC B6K1G6;
DT 01-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 16-OCT-2013, sequence version 2.
DT 03-AUG-2022, entry version 71.
DE RecName: Full=Probable cytosolic Fe-S cluster assembly factor SJAG_02895;
GN ORFNames=SJAG_02895;
OS Schizosaccharomyces japonicus (strain yFS275 / FY16936) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=402676;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=yFS275 / FY16936;
RX PubMed=21511999; DOI=10.1126/science.1203357;
RA Rhind N., Chen Z., Yassour M., Thompson D.A., Haas B.J., Habib N.,
RA Wapinski I., Roy S., Lin M.F., Heiman D.I., Young S.K., Furuya K., Guo Y.,
RA Pidoux A., Chen H.M., Robbertse B., Goldberg J.M., Aoki K., Bayne E.H.,
RA Berlin A.M., Desjardins C.A., Dobbs E., Dukaj L., Fan L., FitzGerald M.G.,
RA French C., Gujja S., Hansen K., Keifenheim D., Levin J.Z., Mosher R.A.,
RA Mueller C.A., Pfiffner J., Priest M., Russ C., Smialowska A., Swoboda P.,
RA Sykes S.M., Vaughn M., Vengrova S., Yoder R., Zeng Q., Allshire R.,
RA Baulcombe D., Birren B.W., Brown W., Ekwall K., Kellis M., Leatherwood J.,
RA Levin H., Margalit H., Martienssen R., Nieduszynski C.A., Spatafora J.W.,
RA Friedman N., Dalgaard J.Z., Baumann P., Niki H., Regev A., Nusbaum C.;
RT "Comparative functional genomics of the fission yeasts.";
RL Science 332:930-936(2011).
CC -!- FUNCTION: Fusion protein of two essential components of the cytosolic
CC iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for
CC maturation of extramitochondrial Fe-S proteins. May form a
CC heterotetramer with nubp35, functioning as a Fe-S scaffold complex,
CC mediating the de novo assembly of an Fe-S cluster and its transfer to
CC target apoproteins (By similarity). {ECO:0000250}.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000250};
CC Note=Binds 4 [4Fe-4S] clusters per heterotetramer. Contains two stable
CC clusters in the N-termini of nbp35 and two labile, bridging clusters
CC between subunits of the nbp35-SJAG_02895 heterotetramer. {ECO:0000250};
CC -!- SUBUNIT: Heterotetramer of 2 nbp35 and 2 SJAG_02895 chains.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm. Nucleus.
CC -!- MISCELLANEOUS: Results from a fusion of two genes coding for two
CC proteins which both play a role in assembly of Fe-S clusters in other
CC species (CFD1 and CIA1 in S.cerevisiae).
CC -!- SIMILARITY: In the N-terminal section; belongs to the Mrp/NBP35 ATP-
CC binding proteins family. NUBP2/CFD1 subfamily. {ECO:0000305}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the WD repeat CIA1
CC family. {ECO:0000305}.
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DR EMBL; KE651166; EEB07787.2; -; Genomic_DNA.
DR RefSeq; XP_002174080.2; XM_002174044.2.
DR AlphaFoldDB; B6K1G6; -.
DR SMR; B6K1G6; -.
DR STRING; 4897.EEB07787; -.
DR EnsemblFungi; EEB07787; EEB07787; SJAG_02895.
DR GeneID; 7048576; -.
DR VEuPathDB; FungiDB:SJAG_02895; -.
DR eggNOG; KOG0645; Eukaryota.
DR eggNOG; KOG3022; Eukaryota.
DR HOGENOM; CLU_030983_0_0_1; -.
DR OMA; IDDDWEC; -.
DR OrthoDB; 793298at2759; -.
DR Proteomes; UP000001744; Unassembled WGS sequence.
DR GO; GO:0097361; C:CIA complex; IEA:InterPro.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-UniRule.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0140663; F:ATP-dependent FeS chaperone activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016226; P:iron-sulfur cluster assembly; IEA:UniProtKB-UniRule.
DR Gene3D; 2.130.10.10; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_03037; ciao1; 1.
DR HAMAP; MF_02040; Mrp_NBP35; 1.
DR HAMAP; MF_03039; NUBP2; 1.
DR InterPro; IPR028608; CIAO1/Cia1.
DR InterPro; IPR019591; Mrp/NBP35_ATP-bd.
DR InterPro; IPR000808; Mrp_CS.
DR InterPro; IPR028600; NUBP2/Cfd1_eukaryotes.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR InterPro; IPR033756; YlxH/NBP35.
DR PANTHER; PTHR23264; PTHR23264; 1.
DR Pfam; PF10609; ParA; 1.
DR Pfam; PF00400; WD40; 5.
DR SMART; SM00320; WD40; 7.
DR SUPFAM; SSF50978; SSF50978; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS01215; MRP; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 4.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 3: Inferred from homology;
KW 4Fe-4S; ATP-binding; Cytoplasm; Iron; Iron-sulfur; Metal-binding;
KW Nucleotide-binding; Nucleus; Reference proteome; Repeat; WD repeat.
FT CHAIN 1..612
FT /note="Probable cytosolic Fe-S cluster assembly factor
FT SJAG_02895"
FT /id="PRO_0000382921"
FT REPEAT 287..326
FT /note="WD 1"
FT REPEAT 330..370
FT /note="WD 2"
FT REPEAT 375..414
FT /note="WD 3"
FT REPEAT 420..459
FT /note="WD 4"
FT REPEAT 464..503
FT /note="WD 5"
FT REPEAT 528..566
FT /note="WD 6"
FT REPEAT 574..612
FT /note="WD 7"
FT BINDING 13..20
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT BINDING 200
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_note="ligand shared between dimeric partners"
FT /evidence="ECO:0000250"
FT BINDING 203
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_note="ligand shared between dimeric partners"
FT /evidence="ECO:0000250"
SQ SEQUENCE 612 AA; 68179 MW; B67BFDDD7728B098 CRC64;
MENVKHIILV LSGKGGVGKS SVTTQLALSL HETPFYSRKL RIGVLDIDLT GPSIPRMFGM
DAETHRIHQS SSGWVPVYTD ETKEIGLMSL AFLLSSKNDS VVWRGPKKAA MIRQFVSDVN
WGDIDYLLID TPPGTSDEHL TIVESLLSVT SERPQLIDGA VMVTTPQNVA TLDVKKEINF
CQNLKIPILG VVENMSGFVC PHCSDCTNIF SKGGGEQLSN TYHLPFLGAV PIDPKFGELI
ESQSTEHPLI EHYKQLEVAK LFQKITQNML DSLFHSPSLV LDKVLSGHRG RIWSVAVHPT
LPLVATASED KSVRVFQAQT GELIHVIDGY HTRSVRRVAW RPIDRPVLAI ASFDATVSIY
EKIDDDWECV AALEGHENEV KCVAWSHDGV YLATCSRDKS VWIWEAMEDD EFDCLAVLQE
HTQDVKVVAW HPKDDLLVSG SYDNTIRFWR DDGDDWVQTC ELTSHTSTVW ALNFSPDGRL
LASGDGEGEV FIWEKLVSNE DAARTPSTNI LRPSLEEEWC LKAVLPRTFT EPVYTLGWKD
DHTLCASGAE GTIGLFAYED DVSTWHTVSL KEKAHDVYEI NTIAWTNDSR LLSGGDDGLC
NVWKLSEADQ TA
