CFI1A_SOYBN
ID CFI1A_SOYBN Reviewed; 218 AA.
AC Q93XE6; C6TDS7;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 97.
DE RecName: Full=Chalcone--flavanone isomerase 1A;
DE Short=Chalcone isomerase 1A;
DE EC=5.5.1.6;
GN Name=CHI1A;
OS Glycine max (Soybean) (Glycine hispida).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine;
OC Glycine subgen. Soja.
OX NCBI_TaxID=3847;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION, AND TISSUE SPECIFICITY.
RX PubMed=15778463; DOI=10.1104/pp.104.054502;
RA Ralston L., Subramanian S., Matsuno M., Yu O.;
RT "Partial reconstruction of flavonoid and isoflavonoid biosynthesis in yeast
RT using soybean type I and type II chalcone isomerases.";
RL Plant Physiol. 137:1375-1388(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Ching-Ren-Woo-Dou; TISSUE=Seed coat;
RA Chiu M.J., Chen S., Wang C.;
RT "Glycine max mRNA for chalcone isomerase.";
RL Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. L66-14;
RA Liao R.-M., Chiu M.-H., Chen S.-H., Chu T.-M., Wang C.-S.;
RT "Molecular cloning of genes encoding two types of chalcone isomerase in
RT soybean (Glycine max).";
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Cheung F., Xiao Y., Chan A., Moskal W., Town C.D.;
RL Submitted (AUG-2009) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the intramolecular cyclization of bicyclic
CC chalcones into tricyclic (S)-flavanones. Responsible for the
CC isomerization of 4,2',4',6'-tetrahydroxychalcone (also termed chalcone)
CC into naringenin. {ECO:0000269|PubMed:15778463}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a chalcone = a flavanone.; EC=5.5.1.6;
CC -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC -!- TISSUE SPECIFICITY: Expressed in roots, shoots, flowers and seeds.
CC {ECO:0000269|PubMed:15778463}.
CC -!- INDUCTION: In roots by B.japonicum. {ECO:0000269|PubMed:15778463}.
CC -!- MISCELLANEOUS: Part of the biosynthetic pathway for all classes of
CC flavonoids, a large class of secondary plant metabolites, many of which
CC are brightly colored.
CC -!- SIMILARITY: Belongs to the chalcone isomerase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ACU19979.1; Type=Miscellaneous discrepancy; Note=Chimeric cDNA. A chimeric cDNA originating from chromosomes 19 and 20.; Evidence={ECO:0000305};
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DR EMBL; AY595413; AAT94358.1; -; mRNA.
DR EMBL; AF276302; AAK69432.1; -; mRNA.
DR EMBL; DQ191401; ABA86740.1; -; Genomic_DNA.
DR EMBL; BT095742; ACU19979.1; ALT_SEQ; mRNA.
DR RefSeq; NP_001235219.1; NM_001248290.1.
DR AlphaFoldDB; Q93XE6; -.
DR SMR; Q93XE6; -.
DR STRING; 3847.GLYMA20G38560.1; -.
DR PRIDE; Q93XE6; -.
DR ProMEX; Q93XE6; -.
DR EnsemblPlants; KRG92975; KRG92975; GLYMA_20G241500.
DR GeneID; 547602; -.
DR Gramene; KRG92975; KRG92975; GLYMA_20G241500.
DR KEGG; gmx:547602; -.
DR eggNOG; ENOG502QR5P; Eukaryota.
DR InParanoid; Q93XE6; -.
DR OMA; NCVAHLK; -.
DR OrthoDB; 1158490at2759; -.
DR BRENDA; 5.5.1.6; 2483.
DR UniPathway; UPA00154; -.
DR Proteomes; UP000008827; Chromosome 20.
DR Genevisible; Q93XE6; GM.
DR GO; GO:0045430; F:chalcone isomerase activity; IEA:UniProtKB-EC.
DR GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 1.10.890.20; -; 1.
DR Gene3D; 3.50.70.10; -; 1.
DR InterPro; IPR044164; CFI.
DR InterPro; IPR016087; Chalcone_isomerase.
DR InterPro; IPR016088; Chalcone_isomerase_3-sand.
DR InterPro; IPR016089; Chalcone_isomerase_bundle_sf.
DR InterPro; IPR036298; Chalcone_isomerase_sf.
DR PANTHER; PTHR28039; PTHR28039; 1.
DR Pfam; PF02431; Chalcone; 1.
DR SUPFAM; SSF54626; SSF54626; 1.
PE 2: Evidence at transcript level;
KW Flavonoid biosynthesis; Isomerase; Reference proteome.
FT CHAIN 1..218
FT /note="Chalcone--flavanone isomerase 1A"
FT /id="PRO_0000300850"
FT BINDING 47
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 112
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 189
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT SITE 105
FT /note="Important for catalytic activity"
FT /evidence="ECO:0000250"
SQ SEQUENCE 218 AA; 23265 MW; 61C6990305849C10 CRC64;
MATISAVQVE FLEFPAVVTS PASGKTYFLG GAGERGLTIE GKFIKFTGIG VYLEDKAVPS
LAAKWKGKTS EELVHTLHFY RDIISGPFEK LIRGSKILPL AGAEYSKKVM ENCVAHMKSV
GTYGDAEAAA IEKFAEAFKN VNFAPGASVF YRQSPDGILG LSFSEDATIP EKEAAVIENK
AVSAAVLETM IGEHAVSPDL KRSLASRLPA VLSHGIIV
