CFI1_CAEEL
ID CFI1_CAEEL Reviewed; 467 AA.
AC O02326; Q8T628;
DT 10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 3.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=AT-rich interactive domain-containing protein cfi-1;
DE AltName: Full=ARID domain-containing protein CFI-1;
GN Name=cfi-1; ORFNames=T23D8.8;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DNA-BINDING, SUBCELLULAR LOCATION,
RP TISSUE SPECIFICITY, AND INDUCTION.
RC STRAIN=Bristol N2;
RX PubMed=11959845; DOI=10.1101/gad.976002;
RA Shaham S., Bargmann C.I.;
RT "Control of neuronal subtype identity by the C. elegans ARID protein CFI-
RT 1.";
RL Genes Dev. 16:972-983(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3] {ECO:0000305}
RP FUNCTION, DEVELOPMENTAL STAGE, AND MUTAGENESIS OF 39-ARG--SER-467.
RX PubMed=28056346; DOI=10.1016/j.neuron.2016.11.036;
RA Kerk S.Y., Kratsios P., Hart M., Mourao R., Hobert O.;
RT "Diversification of C. elegans Motor Neuron Identity via Selective Effector
RT Gene Repression.";
RL Neuron 93:80-98(2017).
CC -!- FUNCTION: Transcription factor (PubMed:11959845, PubMed:28056346).
CC Regulates neuronal subtype identity (PubMed:11959845, PubMed:28056346).
CC Involved in motor neuron fate determination and maintenance, acting as
CC a transcriptional repressor to counteract gene activation by
CC transcription factor unc-3 in a subset of motor neurons
CC (PubMed:28056346). Probably acts by binding to specific promoter
CC elements (PubMed:28056346). Promotes differentiation of URA sensory
CC neurons and prevents them from expressing male-specific CEM neuronal
CC features (PubMed:11959845). Promotes differentiation of AVD and PVC
CC interneurons and their glutamate receptor expression (PubMed:11959845).
CC {ECO:0000269|PubMed:11959845, ECO:0000269|PubMed:28056346}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00355,
CC ECO:0000269|PubMed:11959845}.
CC -!- TISSUE SPECIFICITY: Present in IL2 and URA neurons, and in AVD and PVC
CC interneurons. Present in muscles from head and pharynx (at protein
CC level). {ECO:0000269|PubMed:11959845}.
CC -!- DEVELOPMENTAL STAGE: Expressed in mid-late embryo, throughout larval
CC development and in the adult (PubMed:11959845). Expressed in larvae in
CC various motor neurons, including cholinergic DA, DB, VA, and VB, and
CC also GABAergic DD and VD, but not AS or VC, nor in SAB or DA9 motor
CC neurons (PubMed:28056346). {ECO:0000269|PubMed:11959845,
CC ECO:0000269|PubMed:28056346}.
CC -!- INDUCTION: By unc-86. {ECO:0000269|PubMed:11959845}.
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DR EMBL; AF487547; AAL93258.1; -; mRNA.
DR EMBL; Z81128; CAB03395.3; -; Genomic_DNA.
DR EMBL; AL023824; CAB03395.3; JOINED; Genomic_DNA.
DR EMBL; Z81033; CAB03395.3; JOINED; Genomic_DNA.
DR PIR; T25159; T25159.
DR RefSeq; NP_492644.2; NM_060243.2.
DR AlphaFoldDB; O02326; -.
DR SMR; O02326; -.
DR STRING; 6239.T23D8.8; -.
DR iPTMnet; O02326; -.
DR EPD; O02326; -.
DR PaxDb; O02326; -.
DR PeptideAtlas; O02326; -.
DR PRIDE; O02326; -.
DR EnsemblMetazoa; T23D8.8.1; T23D8.8.1; WBGene00000476.
DR GeneID; 188793; -.
DR KEGG; cel:CELE_T23D8.8; -.
DR UCSC; T23D8.8; c. elegans.
DR CTD; 188793; -.
DR WormBase; T23D8.8; CE31068; WBGene00000476; cfi-1.
DR eggNOG; KOG2744; Eukaryota.
DR GeneTree; ENSGT00940000169348; -.
DR HOGENOM; CLU_567702_0_0_1; -.
DR InParanoid; O02326; -.
DR OMA; NFMHRIG; -.
DR OrthoDB; 1347369at2759; -.
DR PhylomeDB; O02326; -.
DR PRO; PR:O02326; -.
DR Proteomes; UP000001940; Chromosome I.
DR Bgee; WBGene00000476; Expressed in pharyngeal muscle cell (C elegans) and 3 other tissues.
DR GO; GO:0005634; C:nucleus; IDA:WormBase.
DR GO; GO:0003677; F:DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:WormBase.
DR GO; GO:0045165; P:cell fate commitment; IMP:WormBase.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IMP:WormBase.
DR GO; GO:0030182; P:neuron differentiation; IMP:WormBase.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:WormBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 1.10.150.60; -; 1.
DR InterPro; IPR045147; ARI3A/B/C.
DR InterPro; IPR001606; ARID_dom.
DR InterPro; IPR036431; ARID_dom_sf.
DR InterPro; IPR023334; REKLES_domain.
DR PANTHER; PTHR15348; PTHR15348; 1.
DR Pfam; PF01388; ARID; 1.
DR SMART; SM00501; BRIGHT; 1.
DR SUPFAM; SSF46774; SSF46774; 1.
DR PROSITE; PS51011; ARID; 1.
DR PROSITE; PS51486; REKLES; 1.
PE 1: Evidence at protein level;
KW Developmental protein; DNA-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..467
FT /note="AT-rich interactive domain-containing protein cfi-1"
FT /id="PRO_0000295166"
FT DOMAIN 181..273
FT /note="ARID"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00355"
FT DOMAIN 356..464
FT /note="REKLES"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00819"
FT REGION 1..56
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 383..441
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 13..30
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 31..48
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 383..409
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 420..441
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 39..467
FT /note="Missing: In ot786; Glutamate receptor (GluR) gene
FT glr-4 is ectopically expressed in DA and DB motor neurons."
FT /evidence="ECO:0000269|PubMed:28056346"
SQ SEQUENCE 467 AA; 51790 MW; E7F3A5E81963E9E5 CRC64;
MSVRIDEPQL FVSMSKEPTQ ETVNVGGHHD DSSSNCDERV DDQTEEQKSP PASPDLTANL
NVFDLESRQK VVQRLLNSQL NLSNLRAPLN LPPIFQALQG PFSIQQQLLG LASGLTAISP
GLDDYDEENT NQGEPEDLTL GGFRKETSVK SEEPSESGIN ASGPAWSYEE QFKQLYELSD
DVKRKEWLDD WLNFMHRIGK PVTRIPIMAK QVLDLYELYR LVVQHGGLVE IINKKLWREI
TKGLNLPSSI TSAAFTLRTQ YQKYLYDYEC EKEKLSNQSD LQQAIDGNRR EAPGRRTAPS
FPLPFQLPHA ASAAATMLNN QLNGLGMRND LLDDENTLSL QASGLFGTSY GAEQMAILEA
HQRNLERAQR AVQQQVARQS LGLTACSNGN GGNIHNSGRE STSSNDSDIP AKRPKLENDV
KTNGASSMRI STKHSDNSKT SMSVSMEING ITYQGVLFAL DETVSES
