ID   CFI1_SOYBN              Reviewed;         172 AA.
AC   O81980;
DT   15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1998, sequence version 1.
DT   03-AUG-2022, entry version 90.
DE   RecName: Full=Chalcone--flavanone isomerase 1;
DE            Short=Chalcone isomerase 1;
DE            EC=5.5.1.6;
DE   Flags: Fragment;
GN   Name=CHI1;
OS   Glycine max (Soybean) (Glycine hispida).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine;
OC   Glycine subgen. Soja.
OX   NCBI_TaxID=3847;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND INDUCTION.
RC   STRAIN=cv. Williams 82;
RX   PubMed=9869427; DOI=10.1023/a:1006036827841;
RA   Seehaus K., Tenhaken R.;
RT   "Cloning of genes by mRNA differential display induced during the
RT   hypersensitive reaction of soybean after inoculation with Pseudomonas
RT   syringae pv. glycinea.";
RL   Plant Mol. Biol. 38:1225-1234(1998).
CC   -!- FUNCTION: Catalyzes the intramolecular cyclization of bicyclic
CC       chalcones into tricyclic (S)-flavanones. Responsible for the
CC       isomerization of 4,2',4',6'-tetrahydroxychalcone (also termed chalcone)
CC       into naringenin (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a chalcone = a flavanone.; EC=5.5.1.6;
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- INDUCTION: Induced during a hypersensitive response (HR) mediated by
CC       P.syringae glycinea. {ECO:0000269|PubMed:9869427}.
CC   -!- MISCELLANEOUS: Part of the biosynthetic pathway for all classes of
CC       flavonoids, a large class of secondary plant metabolites, many of which
CC       are brightly colored.
CC   -!- SIMILARITY: Belongs to the chalcone isomerase family. {ECO:0000305}.
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DR   EMBL; AJ004902; CAA06202.1; -; mRNA.
DR   PIR; T07657; T07657.
DR   AlphaFoldDB; O81980; -.
DR   SMR; O81980; -.
DR   PRIDE; O81980; -.
DR   InParanoid; O81980; -.
DR   BRENDA; 5.5.1.6; 2483.
DR   UniPathway; UPA00154; -.
DR   Proteomes; UP000008827; Unplaced.
DR   GO; GO:0045430; F:chalcone isomerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 1.10.890.20; -; 1.
DR   Gene3D; 3.50.70.10; -; 1.
DR   InterPro; IPR044164; CFI.
DR   InterPro; IPR016087; Chalcone_isomerase.
DR   InterPro; IPR016088; Chalcone_isomerase_3-sand.
DR   InterPro; IPR016089; Chalcone_isomerase_bundle_sf.
DR   InterPro; IPR036298; Chalcone_isomerase_sf.
DR   PANTHER; PTHR28039; PTHR28039; 1.
DR   Pfam; PF02431; Chalcone; 1.
DR   SUPFAM; SSF54626; SSF54626; 1.
PE   2: Evidence at transcript level;
KW   Flavonoid biosynthesis; Isomerase; Reference proteome.
FT   CHAIN           <1..172
FT                   /note="Chalcone--flavanone isomerase 1"
FT                   /id="PRO_0000166442"
FT   BINDING         2
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         67
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         144
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   SITE            60
FT                   /note="Important for catalytic activity"
FT                   /evidence="ECO:0000250"
FT   NON_TER         1
SQ   SEQUENCE   172 AA;  18744 MW;  7CFE2F9ED91662BE CRC64;
     YTGIGVYLED KAVPSLAAKW KGKTSEELVH TLHFYRDIIS GPFEKLIRGS KILPLAGAEY
     SKKVMENCVA HMKSVGTYGD AEAAAIEKFA EAFKNVNFAP GPLFLYRQSP DGILGLSFSE
     DVTIPEKEAA VIENKAVSAA VLETMIGEHA VSPDLKRILA SRLLRIEHGI IV