ID   CFI1_VITVI              Reviewed;         234 AA.
AC   P51117;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   03-AUG-2022, entry version 97.
DE   RecName: Full=Chalcone--flavanone isomerase 1;
DE            Short=Chalcone isomerase 1;
DE            EC=5.5.1.6;
GN   Name=CHI1;
OS   Vitis vinifera (Grape).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; Vitales; Vitaceae; Viteae; Vitis.
OX   NCBI_TaxID=29760;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Lambrusco Foglia Frastagliata;
RX   PubMed=8193299; DOI=10.1007/bf00029856;
RA   Sparvoli F., Martin C., Scienza A., Gavazzi G., Tonelli C.;
RT   "Cloning and molecular analysis of structural genes involved in flavonoid
RT   and stilbene biosynthesis in grape (Vitis vinifera L.).";
RL   Plant Mol. Biol. 24:743-755(1994).
CC   -!- FUNCTION: Catalyzes the intramolecular cyclization of bicyclic
CC       chalcones into tricyclic (S)-flavanones. Responsible for the
CC       isomerization of 4,2',4',6'-tetrahydroxychalcone (also termed chalcone)
CC       into naringenin (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a chalcone = a flavanone.; EC=5.5.1.6;
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- MISCELLANEOUS: Part of the biosynthetic pathway for all classes of
CC       flavonoids, a large class of secondary plant metabolites, many of which
CC       are brightly colored.
CC   -!- SIMILARITY: Belongs to the chalcone isomerase family. {ECO:0000305}.
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DR   EMBL; X75963; CAA53577.1; -; mRNA.
DR   RefSeq; NP_001268033.1; NM_001281104.1.
DR   AlphaFoldDB; P51117; -.
DR   SMR; P51117; -.
DR   PRIDE; P51117; -.
DR   GeneID; 100233078; -.
DR   KEGG; vvi:100233078; -.
DR   OrthoDB; 798672at2759; -.
DR   UniPathway; UPA00154; -.
DR   ExpressionAtlas; P51117; baseline and differential.
DR   GO; GO:0045430; F:chalcone isomerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 1.10.890.20; -; 1.
DR   Gene3D; 3.50.70.10; -; 1.
DR   InterPro; IPR044164; CFI.
DR   InterPro; IPR016087; Chalcone_isomerase.
DR   InterPro; IPR016088; Chalcone_isomerase_3-sand.
DR   InterPro; IPR016089; Chalcone_isomerase_bundle_sf.
DR   InterPro; IPR036298; Chalcone_isomerase_sf.
DR   PANTHER; PTHR28039; PTHR28039; 1.
DR   Pfam; PF02431; Chalcone; 1.
DR   SUPFAM; SSF54626; SSF54626; 1.
PE   2: Evidence at transcript level;
KW   Flavonoid biosynthesis; Isomerase.
FT   CHAIN           1..234
FT                   /note="Chalcone--flavanone isomerase 1"
FT                   /id="PRO_0000166443"
FT   BINDING         50
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         115
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         192
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   SITE            108
FT                   /note="Important for catalytic activity"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   234 AA;  25140 MW;  6EF1CED2B58358D8 CRC64;
     MSQVPSVTAV QVENVLFPPS VKPPGSTNDL FLGGAGVRGL EIQGKFVKFT AIGVYLENSA
     VPTLAVKWKG KTVEELADSV DFFRDVVTGP FEKFTKVTTI LPLTGRQYSD KVSENCVAFW
     KSVGIYTDAE AKAIEKFNEV LKDETFPPGN SILFTHSPLG ALTMSFSKDG SLPEVGNAVI
     ENKLLTEAVL ESIIGKHGVS PEAKKSLAAR LSELFCKEAG DEKIEAEKVA PVAC