CFI2A_SOYBN
ID CFI2A_SOYBN Reviewed; 226 AA.
AC Q53B74;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 75.
DE RecName: Full=Chalcone--flavanone isomerase 2-A;
DE Short=Chalcone isomerase 2-A;
DE EC=5.5.1.6;
GN Name=CHI2-A; Synonyms=CHI2;
OS Glycine max (Soybean) (Glycine hispida).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine;
OC Glycine subgen. Soja.
OX NCBI_TaxID=3847;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=15778463; DOI=10.1104/pp.104.054502;
RA Ralston L., Subramanian S., Matsuno M., Yu O.;
RT "Partial reconstruction of flavonoid and isoflavonoid biosynthesis in yeast
RT using soybean type I and type II chalcone isomerases.";
RL Plant Physiol. 137:1375-1388(2005).
CC -!- FUNCTION: Catalyzes the intramolecular cyclization of bicyclic
CC chalcones into tricyclic (S)-flavanones. Responsible for the
CC isomerization of 4,2',4',6'-tetrahydroxychalcone (also termed chalcone)
CC into naringenin. {ECO:0000269|PubMed:15778463}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a chalcone = a flavanone.; EC=5.5.1.6;
CC -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC -!- TISSUE SPECIFICITY: Mostly expressed in flowers, and, to a lower
CC extent, in roots, shoots, and seeds. {ECO:0000269|PubMed:15778463}.
CC -!- MISCELLANEOUS: Part of the biosynthetic pathway for all classes of
CC flavonoids, a large class of secondary plant metabolites, many of which
CC are brightly colored.
CC -!- SIMILARITY: Belongs to the chalcone isomerase family. {ECO:0000305}.
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DR EMBL; AY595415; AAT94360.1; -; mRNA.
DR RefSeq; NP_001236768.1; NM_001249839.1.
DR AlphaFoldDB; Q53B74; -.
DR SMR; Q53B74; -.
DR STRING; 3847.GLYMA20G38580.1; -.
DR GeneID; 732546; -.
DR KEGG; gmx:732546; -.
DR eggNOG; ENOG502QR5P; Eukaryota.
DR InParanoid; Q53B74; -.
DR OrthoDB; 1158490at2759; -.
DR BRENDA; 5.5.1.6; 2483.
DR UniPathway; UPA00154; -.
DR Proteomes; UP000008827; Unplaced.
DR GO; GO:0045430; F:chalcone isomerase activity; IEA:UniProtKB-EC.
DR GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 1.10.890.20; -; 1.
DR Gene3D; 3.50.70.10; -; 1.
DR InterPro; IPR044164; CFI.
DR InterPro; IPR016087; Chalcone_isomerase.
DR InterPro; IPR016088; Chalcone_isomerase_3-sand.
DR InterPro; IPR016089; Chalcone_isomerase_bundle_sf.
DR InterPro; IPR036298; Chalcone_isomerase_sf.
DR PANTHER; PTHR28039; PTHR28039; 1.
DR Pfam; PF02431; Chalcone; 1.
DR SUPFAM; SSF54626; SSF54626; 1.
PE 2: Evidence at transcript level;
KW Flavonoid biosynthesis; Isomerase; Reference proteome.
FT CHAIN 1..226
FT /note="Chalcone--flavanone isomerase 2-A"
FT /id="PRO_0000300853"
FT BINDING 49
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 114
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 191
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT SITE 107
FT /note="Important for catalytic activity"
FT /evidence="ECO:0000250"
SQ SEQUENCE 226 AA; 24679 MW; BCD1D29002B584D1 CRC64;
MAFPSVTSVT VENVTFPPTV KPPCSPNTFF LAGAGVRGLQ IHHAFVKFTA ICIYLQYDAL
SFLSVKWKTK STHQLTESDQ FFSDIVTGPF EKFMQVTMIK PLTGQQYSEK VAENCVAIWR
SLGIYTDSEA EAIDKFLSVF KDLTFPPGSS ILFTVSPNGS LTISFSGDET IPEVTSAVIE
NKLLSEAVLE SMIGKNGVSP AAKQSLASRL SHLFKEPGVC DPQSHK
