ID   CFI2B_SOYBN             Reviewed;         227 AA.
AC   A7ISP5;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   11-SEP-2007, sequence version 1.
DT   03-AUG-2022, entry version 56.
DE   RecName: Full=Chalcone--flavanone isomerase 2-B;
DE            Short=Chalcone isomerase 2-B;
DE            EC=5.5.1.6;
GN   Name=CHI2-B;
OS   Glycine max (Soybean) (Glycine hispida).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine;
OC   Glycine subgen. Soja.
OX   NCBI_TaxID=3847;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC   STRAIN=cv. L66-14; TISSUE=Seed coat;
RA   Liao R.-M., Chiu M.-H., Chen S.-H., Chu T.-M., Wang C.-S.;
RT   "Molecular cloning of genes encoding two types of chalcone isomerase in
RT   soybean (Glycine max).";
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the intramolecular cyclization of bicyclic
CC       chalcones into tricyclic (S)-flavanones. Responsible for the
CC       isomerization of 4,2',4',6'-tetrahydroxychalcone (also termed chalcone)
CC       into naringenin (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a chalcone = a flavanone.; EC=5.5.1.6;
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- MISCELLANEOUS: Part of the biosynthetic pathway for all classes of
CC       flavonoids, a large class of secondary plant metabolites, many of which
CC       are brightly colored.
CC   -!- SIMILARITY: Belongs to the chalcone isomerase family. {ECO:0000305}.
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DR   EMBL; DQ191402; ABA86741.1; -; Genomic_DNA.
DR   EMBL; DQ191404; ABA86743.1; -; mRNA.
DR   AlphaFoldDB; A7ISP5; -.
DR   SMR; A7ISP5; -.
DR   PRIDE; A7ISP5; -.
DR   InParanoid; A7ISP5; -.
DR   UniPathway; UPA00154; -.
DR   Proteomes; UP000008827; Unplaced.
DR   GO; GO:0045430; F:chalcone isomerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 1.10.890.20; -; 1.
DR   Gene3D; 3.50.70.10; -; 1.
DR   InterPro; IPR044164; CFI.
DR   InterPro; IPR016087; Chalcone_isomerase.
DR   InterPro; IPR016088; Chalcone_isomerase_3-sand.
DR   InterPro; IPR016089; Chalcone_isomerase_bundle_sf.
DR   InterPro; IPR036298; Chalcone_isomerase_sf.
DR   PANTHER; PTHR28039; PTHR28039; 1.
DR   Pfam; PF02431; Chalcone; 1.
DR   SUPFAM; SSF54626; SSF54626; 1.
PE   2: Evidence at transcript level;
KW   Flavonoid biosynthesis; Isomerase; Reference proteome.
FT   CHAIN           1..227
FT                   /note="Chalcone--flavanone isomerase 2-B"
FT                   /id="PRO_0000314573"
FT   BINDING         50
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         115
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         193
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   SITE            108
FT                   /note="Important for catalytic activity"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   227 AA;  25182 MW;  FD0CACF99CC1BFD4 CRC64;
     MATPASITNV TVEFLQFPAL VTPPGSTKSY FLGGAGVRGL NIQEEFVKFT GIGVYLEDKA
     VSSLAAKWKG KSAAELLDSL DFYRDIIKGP FEKLIRGSKL RTLDGREYVR KVSENCVAHM
     QSVGTYSDEE EKAIEEFRNA FKDQNFPPGS TVFYKQSPTG TLGQLIFSKD ETIPEHEHAV
     IDNKPLSEAV LETMIGEIPV SPALKESLAT RFHQFFKELE ANPNIEN